Microspectroscopic Studies on Ionic Dye Diffusion in Single Ion-Exchange Beads.

单离子交换珠中离子染料扩散的显微光谱研究。

• 批准号: 08640761
• 负责人: KIM Haeng-Boo
• 金额: $ 1.41万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08640761/
• 关键词: ion-exchange; particle diffusion; laser trapping; microspectroscopy; confocal microspectroscopy; microparticle; energy transfer

One of the important factors governing ion-exchange processes is diffusion of an ion in an ion-exchange resin (particle diffusion). Because of a lack of experimental methods, a direct observation of the particle diffusion process is very difficult. Recent progresses in spatially-resolved microspectroscopic techniques such as laser trapping-micro-spectroscopy have provided a powerful means to study various phenomena occurring in microparticles. Here we report direct observation of the particle diffusion process by using laser trapping-microspectroscopy and confocal fluorescence micro-spectroscopy techniques.Two different methods were applied to determine concentration profiles of ionic dyes in single ion-exchange resins. One was confocal fluorescence microspectroscopy, which provided an information on fluorescence intensity profiles along 3D-axs. The fluorescence intensity profile of Rhodamine B (RhB) along the particle diameter, corresponding to the concentration profile of the dye in … More the resin, was measured. From the change in the profile during RhB diffusion, we determined the diffusion coefficient of RhB in the resin. An energy transfer quenching method was also applied. Initially, RhB was pre-adsorbed homogeneously on the ion-exchange resin. Then, Malachite green (MG) as an energy acceptor was adsorbed on the resin. Since MG diffuses from the resin surface to the inside, the RhB fluorescence is quenched only in a MG-diffused layr. The quenching efficiency corresponds to the volume ratio of the MG-diffused layr to the non-diffused layr in the resin, so that the value gives the penetration depth of MG in the resin. On the basis of the change in the fluorescence intensity of RhB during MG diffusion or that in the penetration depth, we determined directly the diffusion coefficient of MG in the ion-exchange resin.The single-particles based spectroscopic techniques provide a useful information on the dynamic processes of the ion-exchange and can e further applied to study photochemical processes in single microparticles. Less

控制离子交换过程的重要因素之一是离子在离子交换树脂中的扩散（颗粒扩散）。由于缺乏实验手段，很难直接观察颗粒扩散过程。近年来，空间分辨显微光谱技术如激光捕获显微光谱技术的发展为研究微粒中的各种现象提供了强有力的手段。本文报道了用激光捕获显微光谱和共聚焦荧光显微光谱技术直接观察颗粒扩散过程，并采用两种不同的方法测定离子染料在单一离子交换树脂中的浓度分布。一种是共焦荧光显微光谱法，其提供关于沿沿着3D轴的荧光强度分布的信息。罗丹明B（RhB）沿着粒径的荧光强度分布对应于染料在纳米颗粒中的浓度分布。 ...更多信息 测量树脂。根据RhB扩散过程中的分布变化，我们确定了RhB在树脂中的扩散系数。还应用了能量转移猝灭方法。最初，RhB被均匀地预吸附在离子交换树脂上。然后，孔雀石绿色（MG）作为能量受体吸附在树脂上。由于MG从树脂表面扩散到内部，因此RhB荧光仅在MG扩散层中被猝灭。猝灭效率对应于树脂中MG扩散层与非扩散层的体积比，因此该值给出了MG在树脂中的渗透深度。根据微球扩散过程中RhB荧光强度的变化或微球穿透深度的变化，直接测定了微球在离子交换树脂中的扩散系数.单粒子光谱技术为研究离子交换的动力学过程提供了有用的信息，并可进一步应用于研究单个微粒中的光化学过程.少

项目成果

N,Kitamura: "Microspectroscopic Analysis of Inhomogeneous Structures in Single Microparticles." The Review of Laser Engineering. 25(11). 754-759 (1997)

N，Kitamura：“单个微粒中不均匀结构的显微光谱分析。”

N.Kitamura: "Photometric Analyses of Optically-Trapped Single Microparticles in Solution." Analytical Sciences. 12 (1). 49-54 (1996)

N.Kitamura：“溶液中光学捕获的单个微粒的光度分析。”

N.Kitamura: "Photometric Analyzes of Optically-Trapped Single Microparticles in Solution." Aalytical Science. 12(1). 49-54 (1996)

N.Kitamura：“溶液中光学捕获的单个微粒的光度分析。”

N.Kitamura: "Photometric Analyses of Optically-Trapped Single Microparticles in Solution." Analytical Science. 12(1). 49-54 (1996)

N.Kitamura：“溶液中光学捕获的单个微粒的光度分析。”

H.-B., Kim: "Effects of Sample Dimension and Dye Distribution Characteristics in Absorption Microspectroscopy." Analytical Chemistry. 70(1). 51-57 (1998)

H.-B.，Kim：“吸收显微光谱中样品尺寸和染料分布特征的影响。”