Microspectroscopic Studies on Sample Dimension and Dye Distribution Characteristics.

样品尺寸和染料分布特征的显微光谱研究。

• 批准号: 10440218
• 负责人: KIM Haeng-Boo
• 金额: $ 6.34万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10440218/
• 关键词: microspectroscopy; microehannel; microparticle; microeapilally; microcapsule; size dependence; sample dimension; 不均一構造; 空間分解分光測定

Models and the relevant equations giving spectroscopic data (optical absorbance, fluorescence intensity, or fluorescence lifetime) of an analyte (I.e., dye) confined in a small volume with different shapes and sizes (sample dimension; D) were given and, factors governing the data were discussed. The spectroscopic properties, particularly dye absorbance, were shown to be dependent on the three-dimensional sample structure: films (1-D), tubes (2-D), and spheres (3-D). The magnitude of the dimension effect on absorbance was determined by the size of both sample and probe beam. Furthermore, dye distribution characteristics in 2-D and 3-D systems also affected absorbance; a dye distributed exclusively to the surface layer of a 2-D or 3-D sample gave an absorbance 0.50 or 0.33-fold of that homogeneously distribution, respectively. If a dye distributed in the inner volume of the sample alone, absorbance increases over that for homogeneous distribution. Effects of sample dimension, dye distribution characteristics, and the size of the probe beam in absorption microspectroscopy were discussed on the basis of the proposed models. Similarly, the sample dimension as well as the dye distribution characteristics affected fluorescence intensity and fluorescence lifetime. For samples such as liquid and polymer films (1-D), microcapillary, microchannel chips, and liquid/liquid interface (2-D), and liquid droplets, polymer beads, microcapsules, giant vesicles (3-D), effects of sample dimension and dye distribution characteristics on the spectroscopic data were discussed in detail.

给出了限制在不同形状和尺寸（样品尺寸；D）的小体积中的分析物（即染料）的光谱数据（光吸光度、荧光强度或荧光寿命）的模型和相关方程，并讨论了控制数据的因素。光谱特性，特别是染料吸光度，被证明取决于三维样品结构：薄膜（1-D）、管（2-D）和球体（3-D）。尺寸对吸光度影响的大小由样品和探测光束的尺寸决定。此外，2-D 和 3-D 系统中的染料分布特性也会影响吸光度；仅分布在 2-D 或 3-D 样品表面层的染料的吸光度分别是该均匀分布的 0.50 或 0.33 倍。如果染料单独分布在样品的内部体积中，则吸光度比均匀分布的吸光度增加。基于所提出的模型，讨论了吸收显微光谱中样品尺寸、染料分布特性和探测光束尺寸的影响。同样，样品尺寸以及染料分布特征影响荧光强度和荧光寿命。对于液体和聚合物薄膜（1-D）、微毛细管、微通道芯片和液/液界面（2-D）以及液滴、聚合物珠、微胶囊、巨型囊泡（3-D）等样品，详细讨论了样品尺寸和染料分布特征对光谱数据的影响。

项目成果

N.Kitamura: "Optical Transformation and Fission of Single Giant Vesicles in Water by Radiation Force." Journal of the American Chemical Society. 120(8). 1942-1943 (1998)

N.Kitamura：“辐射力作用下水中单个巨型囊泡的光学变换和裂变”。

N.Kitamura: "Spectroscopic Analysis of Inhomogeneous Structures in Single Microparticles.in “Mesoscopic Chemistry"" Blackwell Science Ltd.,IUPAC Monograph on the 21st Century Chemistry(in press), (1999)

N.Kitamura：““介观化学”中单微粒不均匀结构的光谱分析”Blackwell Science Ltd.，IUPAC 21 世纪化学专着（正在出版），（1999 年）

N.Kitamura: "Optical Transformation and Fission of Single Giant Vesicles in Water by Radiation Force"Journal of the American Chemical Society. 120(8). 1942-1943 (1998)

N.Kitamura：“辐射力作用下水中单个巨型囊泡的光学变换和裂变”美国化学会杂志。

S.Ishizaki: "Excitation Energy Transfer from Sulforhodamine 101 to Acid Blue 1 at a Liquid/Liquid Interface : Structural Dimension of the Interface"Analytical Chemistry. 71(16). 3382-3389 (1999)

S.Ishizaki：“液/液界面处从磺基罗丹明 101 到酸性蓝 1 的激发能量转移：界面的结构维度”分析化学。

S.Habuchi: "Chemical Size-Effects on Fluorescence Lifetime of Rhodamine 6G in Ethylene Glycopl/Water Microdroplets Dispersed in Polydimethylsiloxane Matrix"Journal of Photochemistry and Photobiology A : Chenistry. (in press). (2000)

S.Habuchi：“分散在聚二甲基硅氧烷基质中的乙二醇/水微滴中的罗丹明 6G 的荧光寿命的化学尺寸效应”光化学和光生物学杂志 A：Chenistry。