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Physiological study on development of heart and its regulatory system during ontogeny in crustaceans

甲壳动物个体发育过程中心脏发育及其调节系统的生理研究

基本信息

批准号：
08640857
负责人：
YAMAGISHI Hiroshi
金额：
$ 0.32万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08640857/
关键词：
Crustacea Ligia exotica heart pacemaker ontogeny heart regulation

项目摘要

The aim of the present study is to elucidate the ontogenetic and phylogenetic diversity of the heartbeat and its regulatory mechanisms in crustaceans. The experiments were performed using histochemical and electrophysiological methods. The results obtained were as follows : 1) We achieved the way to keep the colony of the isopod crustacean Ligia exotica in the laboratory and described its developmental stages. Based on these results, we showed that the heart pacemaker of Ligia exotica is transferred from the heart muscle to the cardiac ganglion during juvenile development and the heartbeat changes from myogenic to neurogenic. Moreover, we showed that the mechanism of the neurogenic heartbeat of the adults is different from that known previously in another crustaceans. 2) We identified one pair of cardioinhibitory and two pairs of cardioacceleratory neurons in the central nervous system of Ligia exotica, and showed that these cardioregulaory neurous innervate both the cardiac ganglion neurons and the myocardium. Moreover, we showed developmental changes in nervous regulation of the heartbeat associatd with the pacemaker transfer during juvenile development. 3) We showed developmental changes in hormonal regulation, especially in aminergic modulation of the heartbeat associated with the pacemaker transfer during juvenile development of Ligia exotica. 4) We found the myogeniic heartbeat in the primitive crustacean Triops longicaudatus and demonstrate first the presence of the myogenic heart in crustacea. We submitted five papers on the above results to professional journals and these papers have been published. We are preparing another some papers on the results.

本研究的目的是阐明甲壳类动物心跳的个体发育和系统发育多样性及其调控机制。实验采用组织化学和电生理学方法。取得的研究结果如下：1)实现了异型等足目甲壳动物Ligia exotica群体的实验室保存，并对其发育阶段进行了描述。根据这些结果，我们认为在幼体发育过程中，外来木贼的心脏起搏器从心肌转移到心脏神经节，心跳从肌源性转变为神经源性。此外，我们还发现，成虫的神经性心跳的机制与以前在另一种甲壳类动物中已知的不同。2)在西洋参的中枢神经系统中发现1对心脏抑制神经元和2对心脏加速神经元，表明这些心脏调节神经元既支配心脏神经节神经元，又支配心肌。此外，我们还显示了与起搏器转移相关的心跳神经调节在青少年发育过程中的发育变化。3)在异色木贼幼体发育过程中，表现出激素调节的发育性变化，尤其是与起搏器转移相关的心跳的胺能调节。4)我们在原始甲壳类动物中发现了肌源性心跳，并首次证明了甲壳类中肌源性心脏的存在。我们就上述结果向专业期刊提交了五篇论文，这些论文已经发表。我们正在准备另一份关于结果的论文。