Isolation of pollen fertility restoration genes in radishes.

本试验旨在分离Ogura雄性不育的Rf基因并鉴定其特性。我们在日本野生萝卜中发现了一个新的Rft基因，它参与了orf138 mRNA的加工，并开发了与Rft紧密连锁的DNA标记。此外，还证明了独特的Rf基因分别分布在野生萝卜、日本野生萝卜和中国栽培萝卜中。测定了它们的DNA序列。在此基础上，对各Rf基因的遗传和进化过程进行了估计。本研究项目总体上显示了野生和栽培萝卜Rf基因的多样性分化。

We conducted the experiments to isolate Rf genes for the Ogura male-sterility and identify their characteristics. We found in Japanese wild radishes new Rft gene that is involved in the processing of mRNA of orf138, and exploited DNA markers tightly linked to the Rft. Furthermore, it was demonstrated that unique Rf genes are distributed in wild species, Raphanus raphanistrum, Japanese wild radish, and Chinese cultivated radishes, respectively. The DNA sequences of them were determined. Based on the results, the genetic and evolutionary processes of establishment of each Rf gene were estimated. This research project shows, as a whole, the diverse differentiations of Rf genes in wild and cultivated radishes.