RFLP MAPPING OF PLASMODIUM REFRACTORY GENES IN MOSQUITOS

据估计，每年有2.8亿人受到影响，100-200万人 死亡是以下疟疾症状的结果 暴露于疟原虫。控制努力一直受到 在疫苗研发和传播方面缺乏进展 抗疟疾药物耐药疟原虫株的研究进展 蚊子媒介对杀虫剂的抗药性，以及 有组织的蚊媒控制方案。的长期目标 拟议的研究是为了识别、分离和表征基因。 与蚊子的易感性和/或耐受性有关 物种，并利用这些信息来开发疟疾控制 基于蚊媒能力遗传破坏的策略。 这个项目的目标是基于这样一个普遍假设： 与疟原虫耐药相关的基因可以被识别和 通过确定它们的遗传连锁关系分离出来的 限制性片段长度多态(RFLP)标记。这项研究 描述了埃及伊蚊及其基因的初步研究 以鸡疟原虫为模式的关系 用于阐明遗传控制机制的系统，因为 关于这种载体的遗传学和 该寄生虫与主要人类的亲缘关系 疟疾寄生虫恶性疟原虫。此外，一些建议的 研究涉及恶性疟原虫冈比亚按蚊的主要媒介。 这个项目的具体目标是：(1)分离影响 疟原虫对Ae.埃及伊蚊使用基于地图的克隆 技术，(2)确定分离的候选基因的潜力 具体目标1积极或消极地影响P。 Ae.中的鸡科真菌埃及伊蚊使用病毒表达系统进行合作 与科罗拉多州立大学合作，(3)研究 寄生虫施加的选择对RFLP等位基因频率随时间的变化 构建了初步的RFLP连锁图谱 对于一个。冈比亚利用为Ae.开发的标记。埃及人在合作中 约翰斯·霍普金斯大学。

Each year, an estimated 280 million people are affected by and 1-2 million deaths occur as a consequence of the symptoms of malaria following exposure to a Plasmodium spp. Control efforts have been limited by the lack of progress in vaccine development, development and spread of Plasmodium strains resistant to antimalarial drugs, development of pesticide resistance in mosquito vectors, and the general decline in organized mosquito vector control programs. The long-term objectives of the proposed research are to identify, isolate, and characterize genes associated with the susceptibility and/or refractoriness of mosquito species, and to utilize this information to develop a malaria control strategy based on the genetic disruption of mosquito vector competence. The objectives of this project are based on the general hypothesis that genes associated with Plasmodium refractoriness can be identified and isolated via determination of their genetic linkage associations with restriction fragment length polymorphism (RFLP) markers. The research described primarily examines the mosquito Aedes aegypti and its genetic relationship with the malarial parasite Plasmodium gallinaceum as a model system for elucidating genetic control mechanisms, because of the wealth of knowledge available concerning the genetics of this vector and the close phylogenetic relationship of this parasite and the major human malarial parasite P. falciparum. Additionally, some of the proposed research involves a primary vector for P. falciparum, Anopheles gambiae. The specific aims of this project are: (1) to isolate genes that influence Plasmodium susceptibility in Ae. aegypti using map-based cloning techniques, (2) determine the potential of candidate genes isolated in specific aim 1 to positively or negatively influence the development of P. gallinaceum in Ae. aegypti using virus expression systems in cooperation with Colorado State University, (3) examine the potential effects of parasite-imposed selection on RFLP allele frequencies over time in laboratory populations, and (4) construct a preliminary RFLP linkage map for An. gambiae using markers developed for Ae. aegypti in cooperation with The Johns Hopkins University.