STUDIES ON THE TRANSCRIPTION FACTOR VP1 INVOLVED IN ABSCISIC ACID RESPOSIVE GENE EXPRESSION

脱落酸反应基因表达转录因子VP1的研究

• 批准号: 08660105
• 负责人: HATTORI Tsukaho
• 金额: $ 1.47万
• 依托单位: MIE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660105/
• 关键词: ABA; abscisic acid; rice; VP1; transcription factor; OSVP1; VIP-C1; two-hybrid; Twn-hybrid; GFP; アラビドプシス

G-box-like response elements (ABREs) have been functionally identified in the promoters of various genes. In addition, single copies of ABRE have been found to require a cis-acting, coupling element to achieve ABA induction. A Coupling Element 3 (CE3) sequence, originally identified as such in the barley HVA1 promoter, is found approximately 30-bp downstream of motif A (G-box-like ABRE) in the promoter of Osem gene. The relationship between these two elements was further defined by linker-scan analyzes of a 55-bp fragment of the Osem promoter, which is sufficient for ABA-responsiveness and VP1 activation. The analyzes revealed that both motif A and CE3 sequence were required not only for ABA-responsiveness but also for VP1 activation. Since the sequences of motif A and CE3 were found to be similar, motif-exchange experiments were carried out. The experiments demonstrated that motif A and CE3 were interchangeable by each other with respect to both ABA and VP1 regulation. Furthermore, both sequences were shown to be recognized by a rice bZIP factor OSBZ8. These results indicate that G-box-like ABREs and CE3 are functionally equivalent cis-acting elements.

G盒样反应元件（ABRE）已在多种基因的启动子中被功能性地鉴定。此外，已发现ABRE的单拷贝需要顺式作用的偶联元件来实现阿坝诱导。偶联元件3（CE 3）序列最初在大麦HVA 1启动子中被鉴定为偶联元件3（CE 3）序列，在Osem基因启动子中基序A（G-box-like ABRE）下游约30-bp处被发现。这两个元素之间的关系进一步定义了一个55 bp的片段的Osem启动子，这是足够的ABA-响应性和VP 1激活的连接子扫描分析。分析表明，基序A和CE 3序列不仅是ABA反应所必需的，而且也是VP 1激活所必需的。由于发现基序A和CE 3的序列相似，因此进行基序交换实验。实验表明，基序A和CE 3是相互互换的阿坝和VP 1的调控。此外，这两个序列被证明是由水稻bZIP因子OSBZ 8识别。这些结果表明，G-box样ABRE和CE 3是功能等同的顺式作用元件。

项目成果

Carson,C.B.: "The guiescent/colorless alleles of viviparousl show That the conserved B3domain of VP1 is not essential for ABA-regulated expression in the seed." Plant J.12. 1231-1240 (1997)

Carson,C.B.：“胎生的隐色/无色等位基因表明 VP1 的保守 B3 结构域对于种子中 ABA 调节的表达并不是必需的。”

Carson, C.B.: "The quiescent/colorless alleles of viviparous/ show that the conserved B3 domain of VP1 is not essential for ABA-regulated expression in the seed." Plant J.12. 1231-1240 (1997)

Carson, C.B.：“胎生/的静止/无色等位基因表明 VP1 的保守 B3 结构域对于种子中 ABA 调节的表达并不是必需的。”

Carson, C.B.Hattori, T., Rosenkrans, L., Vasil, V., Vasil, I.K., PetersonP.P.and McCarty, D.R.: "The quiescent/colorless allele of viviparous1 show that the conservedB3 domain of VP1 is not essential for ABA-regulated gene expression in the seed." Plant J

Carson, C.B.Hattori, T.、Rosenkrans, L.、Vasil, V.、Vasil, I.K.、PetersonP.P. 和 McCarty, D.R.：“viviparous1 的静态/无色等位基因表明 VP1 的保守 dB3 结构域对于