Integration of Regulatory Information for Seed Storage Protein Gene Expression

种子贮藏蛋白基因表达调控信息的整合

基本信息

项目摘要

In this project, we conducted functional analysis of master regulatory transcription factors of seed maturation including VPl/ABI3, FUS3 and LEC1, and ABA responsive element (ABRE)-binding factor TIW31. Through such study, we tried to elucidate the molecular mechanisms underlying the intergration of regulatory information directing the seed-specific expression of seed storage protein genes.By utilizing artificial gene expression induction system with dexamethazone (dex) or estrogen (est), FUS3 or ABI3 was ectopically induced in vegetative tissues of transgenic Arabidopsis plants. As known for ABI3, ectopic expression of FUS3 resulted in the ectopic expression of seed storage protein genes in the presence of ABA. Detailed kinetic analyses of induction Cruciferin C (CRC) by FUS3 was found to be significantly slower than that by ABI3. This and other evidence suggested that ABA-dependent FUS3 induction of CRC was mediated by FUS3 and ABA-dependent synthesis of intermediate regulatory facto … More r (s) (most likely to be banscription factor (s)). Kinetic microarray analysis and cotransfection experiment with cultured cell protoplasts identified bZIP67 transcription factor as the strong candidate for such a intermediate transcription factor.From the analyses using transgeneic Arabidopsis capable of ectopically inducing LEC1, LEC1 regulation of seed storage protein genes was mediated by FUS3 and ABI3. This conclusion was obtained from the observation that ABI3 and FUS3 were induced by ectopic expression of LEC1 and that the LEC1 induction of CRC and At2S expression was significantly recuced in the abi3 or fus3 munatant background.We previously identified ABA-responsive element binding factor TRAB1 as a transcription factor that mediate ABA signal to activated transcription of ABA-regulated genes. In this study, we demonstrated that TRAB1 is activated by ABA-dependent phosphorylation of Serl02. Furhteromre, we identified three SnRK2 protein kinases as those responsible for ABA-dependent phosphorylation of TRAB1. Less
在本项目中,我们对种子成熟的主要调控转录因子VPL/ABI3、FUS3和LEC1以及ABA反应元件(ABRE)结合因子TIW31进行了功能分析。通过这项研究,我们试图阐明调控种子贮藏蛋白基因表达的调控信息整合的分子机制。利用含有地塞米松(Dex)或雌激素(Est)的人工基因表达诱导系统,在转基因拟南芥的营养组织中异地诱导FUS3或ABI3。与ABI3一样,在ABA存在下,FUS3的异位表达导致种子贮藏蛋白基因的异位表达。详细的动力学分析发现,FUS3诱导十字花素C(CRC)的速度明显慢于ABI3。这一证据和其他证据表明,依赖于脱落酸的FUS3对结直肠癌的诱导是由依赖于脱落酸的中间调节因子…的合成介导的更多的r(S)(最有可能是翻译因素(S))。动态基因芯片分析和细胞原生质体共转染实验表明,bZIP67转录因子是这种中间转录因子的有力候选者。通过对能够异位诱导LEC1的转基因拟南芥的分析,LEC1对种子贮藏蛋白基因的调控由FUS3和ABI3介导。这一结论是通过观察到ABI3和FUS3是由LEC1的异位表达诱导的,并且在ABI3或FUS3免疫背景下LEC1诱导的CRC和AT2S的表达显著减少。我们先前发现ABA反应元件结合因子TRAB1是一种转录因子,介导ABA信号激活ABA调节基因的转录。在这项研究中,我们证明了TRAB1是通过ABA依赖的Serl02的磷酸化来激活的。此外,我们鉴定了三个SnRK2蛋白激酶,它们负责依赖于ABA的TRAB1的磷酸化。较少

项目成果

期刊论文数量(44)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Miyoshi, K., Kagaya, Y., Ogawa, Y., Nagato, Y., Hattori, T: "Temporal and spatial expression pattern of the OSVP1 and OSEM genes during seed development in rice"Plant Cell Physiol.. (印刷中). (2002)
Miyoshi, K.、Kagaya, Y.、Okawa, Y.、Nagato, Y.、Hattori, T:“水稻种子发育过程中 OSVP1 和 OSEM 基因的时空表达模式”植物细胞生理学..(出版中) )(2002)。
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Kondo, K., Yamamoto, M., Itahashi, R., Sato, T., Egashira, H., Hattori, T., Kowyama, Y.: "Insights into the evolution of self-compatibility in Lycpersicon from a study of stylar factors."Plant J.. 30. 143-153 (2002)
Kondo, K.、Yamamoto, M.、Itahashi, R.、Sato, T.、Egashira, H.、Hattori, T.、Kowyama, Y.:“从一项研究中深入了解 Lycpersicon 中自我相容性的演变
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Hattori, T.Totsuka, M., Hobo, T., Kagaya, Y., Toyoda-Yamamoto, A.: "Experimentally deremined sequence requirment of ACGT-containing abscisic acid response element."Plant Cell Physiol.. 43. 136-140 (2002)
Hattori, T.Totsuka, M.、Hobo, T.、Kagaya, Y.、Toyoda-Yamamoto, A.:“实验确定含 ACGT 脱落酸反应元件的序列要求。”植物细胞生理学.. 43. 136-
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Kagaya, Y., Hobo, T., Murata, M.Ban, A., Hattori, T.: "Abscisic Acid-Induced Transcription Is Mediated by Phosphorylation of an Abscisic Acid Response Element Binding Factor TRAB1."Plant Cell. 14. 3177-3189 (2002)
Kagaya, Y.、Hobo, T.、Murata, M.Ban, A.、Hattori, T.:“脱落酸诱导的转录是通过脱落酸响应元件结合因子 TRAB1 的磷酸化介导的。”植物细胞。
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Kagaya, Y.: "Abscisic Acid-Induced Transcription Is Mediated by Phosphorylation of an Abscisic Acid Response Element Binding Factor TRAB1"Plant Cell. 14. 3177-3189 (2002)
Kagaya, Y.:“脱落酸诱导的转录是通过脱落酸响应元件结合因子 TRAB1 的磷酸化介导的”植物细胞。
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HATTORI Tsukaho其他文献

HATTORI Tsukaho的其他文献

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{{ truncateString('HATTORI Tsukaho', 18)}}的其他基金

Study on signal transduction mechanisms involved in abscisic acid-responsive transcription
脱落酸响应性转录信号转导机制研究
  • 批准号:
    16380228
  • 财政年份:
    2004
  • 资助金额:
    $ 32.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Studies on Molecular Mechanism of Abscisic Acid-Regulated Transcription
脱落酸调控转录的分子机制研究
  • 批准号:
    11460155
  • 财政年份:
    1999
  • 资助金额:
    $ 32.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
STUDIES ON THE TRANSCRIPTION FACTOR VP1 INVOLVED IN ABSCISIC ACID RESPOSIVE GENE EXPRESSION
脱落酸反应基因表达转录因子VP1的研究
  • 批准号:
    08660105
  • 财政年份:
    1996
  • 资助金额:
    $ 32.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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脱落酸(ABA)对水稻中胚轴的刺激作用及中胚轴伸长突变体的探索
  • 批准号:
    26450017
  • 财政年份:
    2014
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Abscisic acid (ABA) action on plastid transcription in Arabidopsis
脱落酸 (ABA) 对拟南芥质体转录的作用
  • 批准号:
    125910458
  • 财政年份:
    2009
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    $ 32.64万
  • 项目类别:
    Research Grants
Drought tolerance in apple seedlings through abscisic acid (ABA) regulation
通过脱落酸(ABA)调节苹果幼苗的耐旱性
  • 批准号:
    19380025
  • 财政年份:
    2007
  • 资助金额:
    $ 32.64万
  • 项目类别:
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