Studies on liver detoxifying enzyme inducers in food

食品中肝脏解毒酶诱导剂的研究

• 批准号: 08660153
• 负责人: UCHIDA Koji
• 金额: $ 1.41万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660153/
• 关键词: Food components; Liver detoxiying enzymes; Glutathione S-transferase; Rat hepatocytes; Phenolic antioxidants; t-Butylhydroquinone; BHA; Oxiative stress; フェノール性酵酸化剤; セ-ブチルヒドロキノン; 肝解毒酵素; フェノール性抗酸剤; t-ブチルヒドロキノン

Regulation of the GST-Yp transcript has been studied extensively. Recent transgenic rat experiments have revealed that a trans mechanism is involved in the activation of this gene during chemical hepatocarcinogenesis. We found that the promoter of GST gene contains TATA box, TRE (from-61 to -54), sirencer (from -396 to -140). Tretatment of RL34 cells to tBHQ resulted in the changes in the DNA binding activity of several redox-active transcription factors including AP-1. Sp1, and NF-kB.Western blot analysis of these transcription factors revealed that tBHQ induced de novo sysnthesis of these proteins. Taken together, tBHQ and other food-derived inducers may act on the promoter of genes of detoxifying enzymes by way of redox regulationmechanism.

GST-Yp 转录本的调控已被广泛研究。最近的转基因大鼠实验表明，在化学性肝癌发生过程中，反式机制参与了该基因的激活。我们发现GST基因的启动子包含TATA box、TRE（从-61到-54）、sirencer（从-396到-140）。用 tBHQ 处理 RL34 细胞会导致包括 AP-1 在内的几种氧化还原活性转录因子的 DNA 结合活性发生变化。 Sp1 和 NF-kB。这些转录因子的蛋白质印迹分析表明 tBHQ 诱导这些蛋白质的从头合成。综上所述，tBHQ和其他食物源性诱导剂可能通过氧化还原调节机制作用于解毒酶基因的启动子。

项目成果

Fukuda et al.: "Oxidative stress response in iron-induced renal corcnoginesis" Arch.Biochem.Biophys.329. 39-46 (1996)

Fukuda 等人：“铁诱导的肾癌中的氧化应激反应”Arch.Biochem.Biophys.329。

A.Fukuda: "4-Hydroxy-2-nonenal cytotoxicity in renal proximal tubular cells" Arch.Biochem.Biophys.333. 419-426 (1996)

A.Fukuda：“肾近端肾小管细胞中的 4-羟基-2-壬烯醛细胞毒性”Arch.Biochem.Biophys.333。

Y.Hattori: "Formation of 8-hydroxy-2-deoxyguanosive in epidermal cells of hair less mice after chronic UV exposure" J.Invest.Dermatol.107. 733-737 (1996)

Y.Hattori：“慢性紫外线照射后无毛小鼠表皮细胞中 8-羟基-2-脱氧鸟苷的形成”J.Invest.Dermatol.107。

Fukuda et al.: "4-Hydroxy-2-nonenul cytotoxicity in renal proximal tubulas cells." Arch.Biochem.Biophys.333. 419-426 (1996)

Fukuda 等人：“肾近端小管细胞中的 4-Hydroxy-2-nonenul 细胞毒性。”

Fukuda et al.: "Cellulan response to He redoxactive lipid peroxidation Praluts." Biochem.Biophys.Res. Commun.236. 505-509 (1997)

Fukuda 等人：“纤维素对 He 氧化还原脂质过氧化 Praluts 的反应。”