Studies on liver detoxifying enzyme inducers in food

GST-Yp转录本的调控已被广泛研究。最近的转基因大鼠实验表明，在化学性肝癌发生过程中，该基因的激活涉及一种反式机制。我们发现GST基因的启动子包含TATA box、TRE（从-61到-54）、sirencer（从-396到-140）。RL34细胞经thbhq处理后，包括AP-1在内的几种氧化还原活性转录因子的DNA结合活性发生了变化。Sp1和NF-kB。对这些转录因子的Western blot分析显示，thbhq诱导了这些蛋白的重新合成。综上所述，thbhq等食源性诱导剂可能通过氧化还原调控机制作用于解毒酶基因启动子。

Regulation of the GST-Yp transcript has been studied extensively. Recent transgenic rat experiments have revealed that a trans mechanism is involved in the activation of this gene during chemical hepatocarcinogenesis. We found that the promoter of GST gene contains TATA box, TRE (from-61 to -54), sirencer (from -396 to -140). Tretatment of RL34 cells to tBHQ resulted in the changes in the DNA binding activity of several redox-active transcription factors including AP-1. Sp1, and NF-kB.Western blot analysis of these transcription factors revealed that tBHQ induced de novo sysnthesis of these proteins. Taken together, tBHQ and other food-derived inducers may act on the promoter of genes of detoxifying enzymes by way of redox regulationmechanism.