An epidemiological surveillance of lyme borreliosis in wild rodents : especially analysis of the prevalence of the prevalence of harboring organisms and the system of passing the winter

野生啮齿动物莱姆疏螺旋体病的流行病学监测：特别是对寄居微生物的流行情况和越冬系统的分析。

• 批准号: 08660377
• 负责人: SUGIYAMA Yoshihiro
• 金额: $ 1.47万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660377/
• 关键词: Japanese rodeut; Lyme borreliosis; ProteinG-ELISA; Isolation of bacteria; Epidemiology; ライム病; 継続的疫学調査; 抗原捕獲検出法; PCR法; モノクローナル抗体

To obtained epidemiological data of Lyme borreliosis about Japanese wild animals, we carried out the serological surveillance and the isolation of organism from wild rodents that were caught at fixed place in mountain area of northern Ibaraki prefecture throughout the year. Two hundred eighty one of Apodemus speciosus and 60 of Microtus montebelli were caught and tested. As the immunoglobulin of these rodents did react with protein G,we attempted a serological surveillance by the PG-ELISA method using 4 borrelia genotype antigens. The other wild animal serum was also tested by this improved PG-ELISA.The antibody positive rates to Apodemus speciosus and Microtus montebelli were 10.3% and 21.6%, respectively. The positive sera indicating high titer were merely 6.9% in Apodemus speciosus, but 46.2% in Microtus montebelli. At the monthly prevalence, the lowest levels of mean of OD values was found in February. The mean of OD values was gradually rising from May until July. These were some … More serum samples indicating high titer from July to October. After October, there was no serum samples indicating so high titer and the mean was gradually going down until December. The fact supports that Lyme borreliosis antibody in small rodents is related with the tick activity, breeding season and migratory bird etc. And it suggests that these rodents have not so long kept the antibodies and that the size was an important facter for the possession of antibody in Apodemus speciosus. The isolation of organisms were successful from these rodents and these isolation ratio at Apodemus speciosus and Microtus montebelli were 2.1% and 5.0%, respectively. The identification of isolates was carried out by the polyclonal antibody assay, PCR and RFLP.The experimental infection was carried out at low temperature. It was found that there was no difference of keeping organisms at low temperature. It indicated that this organisms were not so long keeping in tick, but keeping long term in animals at low temperature. Therefore, the organisms may be kept in the body of mammals in winter. These study was presented at a oral presentation and a paper, and should be done at two paper. Less

为获得日本野生动物莱姆病流行病学资料，我们对茨城县北部山区固定地点捕获的野生啮齿动物进行了全年血清学监测和生物分离。共捕获斑点姬鼠181只，蒙特贝利鼠60只。由于这些啮齿动物的免疫球蛋白与蛋白G发生反应，我们尝试用4种伯氏疏螺旋体基因型抗原采用PG-ELISA法进行血清学监测。其他野生动物血清也采用改进的PG-ELISA法进行检测。对斑姬鼠和蒙特贝利鼠抗体阳性率分别为10.3%和21.6%。高滴度阳性血清在斑姬鼠中仅为6.9%，在蒙特贝利鼠中为46.2%。在月流行率中，平均OD值在2月份最低。5 ~ 7月OD值均值逐渐升高。这是更多的血清样本显示七月到十月间的高滴度10月以后，没有血清样本显示如此高的滴度，直至12月平均值逐渐下降。结果表明，小鼠莱姆病抗体与蜱虫活动、繁殖季节、候鸟等因素有关。这表明，这些啮齿类动物保存抗体的时间不长，而体型是阿姬鼠拥有抗体的一个重要因素。从鼠体中成功分离出生物，在斑姬鼠和蒙特贝利田鼠中分离率分别为2.1%和5.0%。采用多克隆抗体、PCR和RFLP对分离株进行鉴定。实验感染是在低温下进行的。结果表明，低温贮藏与低温贮藏之间并无差异。这表明该菌不是在蜱虫体内长期存在，而是在动物体内低温长期存在。因此，这些微生物可以在冬季保存在哺乳动物体内。这些研究以口头报告和论文的形式提出，并应在两篇论文中完成。少

项目成果

Y.Sugiyama et al.: "Serological surveillance of Lyme borreliosis in wild Japanese serows." J.Vet.Med.Sci.Vol.60, No6.(in press.). (1998)

Y.Sugiyama 等人：“野生日本鬣羚莱姆疏螺旋体病的血清学监测。”

Y, Sugiyama.er al.: "Serological Surveillance of Lyme borreliosis in wid Japanese Serows." J.Vet.Med.Sci.60(in press). (1998)

Y，Sugiyama.er al.：“日本羚羊莱姆疏螺旋体病的血清学监测”。

YOSHIHIRO SUGIYAMA: "Serological Surveillance of Lyme Borreliosis in Wild Japanese Serows" J.Vet.Med.Sci.60.6(印刷中). (1998)

YOSHIHIRO SUGIYAMA：“野生日本羚羊莱姆疏螺旋体病的血清学监测”J.Vet.Med.Sci.60.6（出版中）。