The three-dimensional structure and the function of the intracellular secretory apparatus

细胞内分泌器的三维结构和功能

• 批准号: 08670018
• 负责人: SUZAKI Etsuko
• 金额: $ 1.47万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670018/
• 关键词: The Golgi apparatus; Lectins; Multiple fluorescence stainings; Confocal laser scanning microscope; Three-dimensional structure; Mouse Brunner's gland cells

The three-dimensional structure of the intracellular secretory components, especially the Golgi apparatus, was visualized in the mouse Brunner'S gland cells by using multiple stainings of fluorescently labeled lectins and a confocal laser scanning microscope (CLSM). In order to distinguish the Golgi stacks of the cis-side, the intermediate, and the trans-side, three lectins were used each of which recognizes the sugar residue added at the right stacks.Based on our observations, we propose a new three-dimensional model of the Golgi apparatus : the Golgi apparatus forms a large dome over a nucleus, the lacy structure of the cis-stacks forms its outer boundary, and its inner surface is lined with intermediate stacks which also form a lacy network and is further paved with trans-stackes. Secretory granules formed from the trans-side are released into the internal space of the Golgi apparatus and then transported to the apical region through the holes of the lacy network of the Golgi stacks.The three-dimensional structure of the Golgi apparatus seems to be the case in other cells which are actively synthesizing mucus. Therefore, we are planning to further investigate the three-dimensional structure of the secretory apparatus by using a CLSM and other new technologies.

利用荧光标记凝集素的多重染色和共聚焦激光扫描显微镜（CLSM），在小鼠布鲁纳腺细胞中观察到细胞内分泌成分，特别是高尔基体的三维结构。为了区分顺式、中间体和反式的高尔基基团，使用了三种凝集素，每一种凝集素都能识别添加在正确基团上的糖残基。根据我们的观察，我们提出了一个新的高尔基体的三维模型：高尔基体在核上形成一个大圆顶，顺堆叠的花边结构形成其外边界，其内表面内衬中间堆叠，中间堆叠也形成一个花边网络，并进一步铺设反堆叠。由外侧形成的分泌颗粒被释放到高尔基体的内部空间，然后通过高尔基体的网状孔输送到根尖区域。高尔基体的三维结构似乎在其他积极合成黏液的细胞中也是如此。因此，我们计划利用CLSM和其他新技术进一步研究分泌器官的三维结构。

项目成果

洲崎 悦子: "ゴルジ装置の三次元構造解析" レーザ顕微鏡研究会第19回講演会論文集. 1-4 (1997)

Etsuko Susaki：“高尔基体的三维结构分析”激光显微镜学会第 19 届会议记录 1-4 (1997)。

Etsuko Suzaki: "Three-dimensional visualization of the Golgi apparatus : Studies on Brunner's gland by a confocal laser scanning microscope" Histochemicstry and Cytochemicstry 1996, Proceedings of the 10th International Congress. 551-552 (1996)

Etsuko Suzaki：“高尔基体的三维可视化：通过共焦激光扫描显微镜对布伦纳腺的研究”组织化学和细胞化学 1996 年，第十届国际大会论文集。

洲崎悦子: "ゴルジ装置の三次元構造解析:コンフォーカル顕微鏡を用いたマウス十二指腸腺細胞の観察" 日本機械学会100周年記念 第10回バイオエンジニアリング講演会講演論文集. 462-463 (1998)

Etsuko Suzaki：“高尔基体的三维结构分析：使用共焦显微镜观察小鼠十二指肠腺细胞”第 10 届生物工程会议记录，日本机械工程师学会成立 100 周年 462-463（1998 年）。

Etsuko Suzaki: "Video-rate dynamics of exocytotic events associated with phagocytosis in neutrophil" Cell Motility and the Cytoskeleton. 38. 215-228 (1997)

Etsuko Suzaki：“与中性粒细胞吞噬作用相关的胞吐事件的视频速率动态”细胞运动和细胞骨架。

Etsuko Suzaki: "Video-rate dynamics of exocytotic events associated with phagocytosis in neutrophils"Cell Motility and the Cytoskeleton. 38. 215-228 (1997)

Etsuko Suzaki：“与中性粒细胞吞噬作用相关的胞吐事件的视频速率动态”细胞运动和细胞骨架。