Regulation of PP2Cbeta gene expression-testis specific expression and multiple promoter

PP2Cbeta基因表达的调控——睾丸特异性表达和多启动子

基本信息

  • 批准号:
    08670133
  • 负责人:
  • 金额:
    $ 1.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1997
  • 项目状态:
    已结题

项目摘要

Out of known 5 isoforms of PP2Cbeta, 3 isoforms, beta-3, -4 and -5, are distinctively expressed in testis and intestine. There has been reports on the variation of 5' end of the PP2Cbeta cDNAs, assigned as type A and B.Also, a promoter (P1), which enables ubiquitous expression to the gene, was recently brought to light at 5' of type A region. In order to investigate the possible variations of the 5' end of the PP2Cbeta mRNAs, 5'-RACE and cloning of the gene of that region were performed, and 3 exons were identified : type A as exon1, type B as exon 3 and exon 2 as a novel one. RT-PCR and Southern blotting analysis showed the exon 2 is expressed only in testis, intestine and liver. Based on these results, 5' region of the exon 2 was further investigated as a potential organ-specific promoter (P2). Multiple transcription start sites were observed by the primer extention experiment, and several SRY and NRE of c-mos consensus sequences were observed, implying the possible involvement of the P2 in sexual differentiation and testicular function. Luciferase reporter assay with P19 EC cells proved significant promoter activity of P2.
在已知的5种PP2Cbeta亚型中,β -3、-4和-5亚型在睾丸和肠道中特异性表达。已经有关于pp2beta cdna 5‘端变异的报道,被分配为A型和b型。此外,最近在A型区域的5’处发现了一个启动子(P1),该启动子使该基因能够普遍表达。为了研究PP2Cbeta mrna 5′端可能的变异,我们对该区域的5′-RACE基因进行克隆,鉴定出3个外显子:A型为外显子1,B型为外显子3,2为新外显子。RT-PCR和Southern blotting分析显示,外显子2仅在睾丸、肠道和肝脏中表达。基于这些结果,我们进一步研究了外显子2的5'区域作为潜在的器官特异性启动子(P2)。引物延伸实验观察到多个转录起始位点,并观察到c-mos一致序列的多个SRY和NRE,暗示P2可能参与性别分化和睾丸功能。P19 EC细胞荧光素酶报告试验证实P2启动子活性显著。

项目成果

期刊论文数量(27)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yoji Sasahara: "Okadaic acid suppresses neural differentiation-dependent expression of the neurofila-ment-Lgene in P19 embryonal carcinoma cells by posttranscriptional modification." J.Biol.Chem.271. 25950-25957 (1996)
Yoji Sasahara:“冈田酸通过转录后修饰抑制 P19 胚胎癌细胞中神经丝 L 基因的神经分化依赖性表达。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Shunsuke, Kato: "Differentiation-dependent enhanced expression of phosphatase 2Cβ in germ cells of mouse seminiferous tubules." FEBS Lett.396. 293-297 (1996)
Shunsuke, Kato:“小鼠生精小管生殖细胞中磷酸酶 2Cβ 的分化依赖性增强表达。”FEBS Lett.393-297 (1996)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Ohnishi, M., Nakagawara, K., Mori, M., Kobayashi, T., Kato, S., Sasahara, Y., Kusuda, K., Chida, N., Kobayashi, T., Yanagawa, Y., Hiraga, A., Takeuchi, T.& Tamura, S.: "Localization of the Mouse protein serine/threonine phosphatase 2Cbeta gene to chromoso
大西,M.,中河原,K.,森,M.,小林,T.,加藤,S.,笹原,Y.,楠田,K.,千田,N.,小林,T.,柳川,Y.,
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yick Pang Ching: "Specificity of different isoforms of protein phosphatase 2A and protein phosphatase 2C studied using site-directed mutagenesis of HMG-CoA reductase." FEBS Lett.411. 265-268 (1997)
Yick Pang Ching:“利用 HMG-CoA 还原酶的定点诱变研究了蛋白磷酸酶 2A 和蛋白磷酸酶 2C 不同亚型的特异性。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

TAMURA Shinri其他文献

TAMURA Shinri的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('TAMURA Shinri', 18)}}的其他基金

Regulation of stress activated protein kinase pathway by protein phosphatase 2C
蛋白磷酸酶 2C 对应激激活蛋白激酶途径的调节
  • 批准号:
    22590280
  • 财政年份:
    2010
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular mechanisms of response of living body to environmental stress
生命体对环境应激反应的分子机制
  • 批准号:
    14370050
  • 财政年份:
    2002
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular mechanism of response of living body to environmental stress
生命体对环境应激反应的分子机制
  • 批准号:
    10470037
  • 财政年份:
    1998
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Joint Study on the Mechanism of Insulin Action
胰岛素作用机制的联合研究
  • 批准号:
    07044218
  • 财政年份:
    1995
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for international Scientific Research

相似海外基金

Vector engineering for non-viral delivery of large genomic DNA to the RPE
用于将大基因组 DNA 非病毒传递至 RPE 的载体工程
  • 批准号:
    10667049
  • 财政年份:
    2023
  • 资助金额:
    $ 1.41万
  • 项目类别:
Functional characterization of genomic DNA elements regulating epigenetic silencing
调节表观遗传沉默的基因组 DNA 元件的功能表征
  • 批准号:
    489367
  • 财政年份:
    2023
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Operating Grants
Effects of heterogeneity of novel genomic DNA modifications in bacteria on host response.
细菌中新型基因组 DNA 修饰的异质性对宿主反应的影响。
  • 批准号:
    23K18230
  • 财政年份:
    2023
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
Novel role of non-coding RNAs in regulating genomic DNA methylation
非编码RNA在调节基因组DNA甲基化中的新作用
  • 批准号:
    2749901
  • 财政年份:
    2022
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Studentship
Genomic DNA deamination editing mechanism regulated by RNA in human
人类RNA调控的基因组DNA脱氨编辑机制
  • 批准号:
    22H02548
  • 财政年份:
    2022
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Incorporating geography into statistical methods for analysis of population genomic DNA
将地理学纳入群体基因组 DNA 分析的统计方法
  • 批准号:
    10737747
  • 财政年份:
    2022
  • 资助金额:
    $ 1.41万
  • 项目类别:
Incorporating geography into statistical methods for analysis of population genomic DNA
将地理学纳入群体基因组 DNA 分析的统计方法
  • 批准号:
    10615605
  • 财政年份:
    2022
  • 资助金额:
    $ 1.41万
  • 项目类别:
Genomic DNA stabilization by novel mechanism of DNA damage tolerance
通过 DNA 损伤耐受的新机制稳定基因组 DNA
  • 批准号:
    21K12241
  • 财政年份:
    2021
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of a novel approach for inhibition of nuclear translocation of HBV genomic DNA in infected cells
开发一种抑制感染细胞中 HBV 基因组 DNA 核易位的新方法
  • 批准号:
    21K15958
  • 财政年份:
    2021
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Principles of chromatin operations ensuring the repair of genomic DNA damage caused by environmental stresses
确保修复环境压力引起的基因组 DNA 损伤的染色质操作原理
  • 批准号:
    21H03598
  • 财政年份:
    2021
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了