RB-E2F system in the progression and evolution of squamous cell carcinoma of the uterine cervix

与细胞增殖调控机制有关的RB-E2F系统的改变被认为参与了人类鳞状细胞癌（SCC）的发生演变。在本研究中，我们检测了24例宫颈鳞状细胞癌RB蛋白的异常表达，以及HPV-16或HPV-18的E6和E7基因的参与。采用定量RT-PCR方法比较11例肺鳞癌组织与正常肺组织中E2F-1 mRNA的表达。HPV 16-E6和E7基因的检出率分别为87.5%和71.4%。免疫组化检测RB蛋白阳性率分别为80.9%和86.6%。与HPV-16相比，HPV-18的检测水平较低。检测E2F-1 mRNA， SCC组织中总mRNA为5.3(]SY.+- . [)3.8fg/ng，总mRNA为1.2(]SY.+- . [)0.5fg/ng，提示E2F系统异常过度表达。RB蛋白在正常组织中呈阴性，在SCC组织中呈持续阳性。推测RB蛋白积累-E2F的过度表达与人宫颈及肺源性鳞状细胞癌的发生发展密切相关。

The alteration of RB-E2F system which is concerned with regulatory mechanism of cell proliferation is supposed to be involved in the progression and evolution of human squamous cell carcinoma (SCC). In the present study, we examined the abnormal expression of RB protein, and the involvement of E6 and E7 genes of HPV-16 or HPV-18 in 24 cases of SCC of the uterine cervix. In addition, E2F-1 mRNA was comparatively measured in 11cases of lung SCC tissue and normal lung tissue by quantitative RT-PCR.HPV 16-E6 and E7 genes were identified 87.5% and 71.4% respectively. RB protein was positively detected at 80.9% and 86.6% by immunohsitochemistry. HPV-18 was detected at the low level as compared with HPV-16. E2F-1 mRNA was measured, showing 5.3(]SY.+-。[)3.8fg/ng total mRNA in SCC tissue and 1.2(]SY.+-。[)0.5fg/ng total mRNA,suggestive of abnormal excessive expression of E2F system. RB protein was negative in normal tissue and constantly positive in SCC tissue. It is presumed that RB protein accumulation -E2F excessive expression would be strongly related to the development of human squamous cell carcinoma derived from the uterine cervix as well as the lung.