RB-E2F system in the progression and evolution of squamous cell carcinoma of the uterine cervix

RB-E2F系统在宫颈鳞状细胞癌进展和演变中的作用

• 批准号: 08670197
• 负责人: NAKANISHI Isao
• 金额: $ 1.22万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670197/
• 关键词: Squamouma cell carcinoma; HPV; RB-E2F

The alteration of RB-E2F system which is concerned with regulatory mechanism of cell proliferation is supposed to be involved in the progression and evolution of human squamous cell carcinoma (SCC). In the present study, we examined the abnormal expression of RB protein, and the involvement of E6 and E7 genes of HPV-16 or HPV-18 in 24 cases of SCC of the uterine cervix. In addition, E2F-1 mRNA was comparatively measured in 11cases of lung SCC tissue and normal lung tissue by quantitative RT-PCR.HPV 16-E6 and E7 genes were identified 87.5% and 71.4% respectively. RB protein was positively detected at 80.9% and 86.6% by immunohsitochemistry. HPV-18 was detected at the low level as compared with HPV-16. E2F-1 mRNA was measured, showing 5.3(]SY.+-。[)3.8fg/ng total mRNA in SCC tissue and 1.2(]SY.+-。[)0.5fg/ng total mRNA,suggestive of abnormal excessive expression of E2F system. RB protein was negative in normal tissue and constantly positive in SCC tissue. It is presumed that RB protein accumulation -E2F excessive expression would be strongly related to the development of human squamous cell carcinoma derived from the uterine cervix as well as the lung.

Rb-E2F系统的改变与细胞增殖的调控机制有关，可能与人鳞状细胞癌的发生、发展有关。在本研究中，我们检测了24例宫颈鳞癌组织中Rb蛋白的异常表达，以及HPV-16或HPV-18的E6和E7基因的参与情况。另外，采用定量RT-PCR法对11例肺鳞状细胞癌组织和正常肺组织中的E2F-1mRNA进行了检测，HPV16-E6和E7基因的符合率分别为87.5%和71.4%。免疫组化检测Rb蛋白阳性率分别为80.9%和86.6%。与HPV-16相比，HPV-18的检测水平较低。E2F-1mRNA检测结果为5.3(SY.+-)。[)3.8fg/ng鳞状细胞癌组织总mRNA和1.2(]Sy.+-。[]0.5fg/ng总mRNA，提示E2F系统异常过度表达。Rb蛋白在正常组织中为阴性，在鳞癌组织中为持续阳性。推测Rb蛋白积聚-E2F过度表达与人宫颈和肺鳞状细胞癌的发生发展密切相关。