Neurochemical studies on the interaction cocaine and alcohol

可卡因和酒精相互作用的神经化学研究

• 批准号: 08670498
• 负责人: UEMURA Koichi
• 金额: $ 0.9万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670498/
• 关键词: Alcohol preference; Cocaine; Mouse; Monoamine; Alcohol metabolism

Concurrent use of alcohol and other drugs is a serious problem. Cocaine is the most frequently abused drug overseas. An increasing number of cases demonstrating concurrent use of alcohol and cocaine is being noted. Alcohol preference (AP) in animals is an index of human drinking behavior. In this study brain monoamine and alcohol metabolism were studied to clarify the mechanisms of change in AP following single or repeated cocaine administration.To inbred strain of mouse, C57BL/6J,10% alcohol and tap water were given and AP was measured following intraperitoneal cocaine administration once or repeatedly for one week. Then the rate of blood alcohol disappearance in vivo (beta value) and liver alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activity in vitro were measured. Changes in brain norepinephrine, dopamine, serotonin and their metabolites were also measured.A single cocaine administration reduced the AP of C57BL/6J mice. beta value was not changed after pretreatment with cocaine. The level of brain dopamine was increased only two hours after cocaine adiministration. In repeated cocaine administration, AP was reduced after cocaine administration. Brain monoamine levels were not changed. Neither the beta value nor liver ADH,ALDH activity was changed after either single or repeated cocaine administration.It is speculated that reduced AP is associated with an increase in brain dopamine and changes in AP did not contribute to alcohol metabolism pharmacologically. Dopaminergic system makes a rule of, in part, alcohol drinking behavior.

同时使用酒精和其他药物是一个严重的问题。可卡因是海外最常滥用的药物。人们注意到，同时使用酒精和可卡因的案例越来越多。动物的酒精偏好（AP）是人类饮酒行为的一个指标。本研究以C57 BL/6 J近交系小鼠为研究对象，通过单次或多次腹腔注射可卡因，测定其脑内单胺和乙醇代谢，探讨可卡因引起AP变化的机制。测定体内血液酒精消失率（β值）和体外肝脏乙醇脱氢酶（ADH）、乙醛脱氢酶（ALDH）活性。结果表明，单次给予可卡因可明显降低C57 BL/6 J小鼠的AP。可卡因预处理后β值无变化。可卡因给药后2小时脑内多巴胺水平升高。在重复可卡因给药中，可卡因给药后AP降低。脑单胺水平没有变化。单次或多次可卡因给药后β值和肝脏ADH、ALDH活性均无变化，推测AP降低与脑内多巴胺增加有关，AP的变化对酒精代谢无贡献。多巴胺能系统在一定程度上决定了饮酒行为。

项目成果

K.Uemura, Y.-J.Li, Y.Ohbora, T.Fujimiya, S.Komura: "Effects of Repeated Cocaine Administration on Alcohol Consumption." Journal of Studies on Alcohol. 59. 115-118 (1998)

K.Uemura、Y.-J.Li、Y.Ohbora、T.Fujimiya、S.Komura：“重复使用可卡因对酒精消费的影响”。

上村 公一、李 裕江、古村 節男: "アルコール嗜好性に及ぼすコカインの急性効果について." アルコールと医学生物学. 16. 120-123 (1996)

Koichi Uemura、Yue Lee 和 Setsuo Furumura：“论可卡因对酒精偏好的急性影响。” 16. 120-123 (1996)。

K.Uemura, Y.-J.Li, Y.Ohbora, T.Fujimiya, S.Komura: "Effects of Repeated Cocaine Administration on Alcohol Consumption." Journal of Studies on Alcohol. 59・1. 115-118 (1998)

K.Uemura、Y.-J.Li、Y.Ohbora、T.Fujimiya、S.Komura：“反复服用可卡因对酒精消费的影响”，《酒精研究杂志》59·1（1998）。