The effect of the central nervous system on apoptosis in the rat intestine

中枢神经系统对大鼠肠道细胞凋亡的影响

• 批准号: 08670601
• 负责人: FUJIMOTO Kazuma
• 金额: $ 1.6万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670601/
• 关键词: hypothalamus; mucosal growth; circadian variation; cell death

We previously demonstrated that the central nervous system playd an important role in the cell growth of the rat intestinal mucosa. The aim of the present study was to examine if the central nervous system had any effect on apoptosis of the intestinal mucosa in addition to the cell growth. The rate of apoptosis was evaluated by the % fragmented DNA in the mucosa. After 24 h fasting, apoptosis increased in the intestinal mucosa. Percent fragmented DNA in the ad libitum feeding rat had a circadian variation which decreased in a dark period when the rat fed. The circadian variation of the intestinal apoptosis had a mirror image of the intestinal ornithine decarboxylase activity. Administration of 2-deoxy-D-glucose into the rat third cerebroventricle tended to decrease apoptosis in the rat intestinal mucosa. This effect was, at least in part, through the lateral hypothalamus. These results suggested the possibility that the central nervous system might control apoptosis in the rat intestinal mucosa.

我们以前证明中枢神经系统在大鼠肠粘膜细胞生长中起重要作用。本研究的目的是检查中枢神经系统是否有任何影响，除了细胞生长的肠粘膜细胞凋亡。通过粘膜中DNA片段化百分比来评价细胞凋亡率。禁食24 h后，肠黏膜细胞凋亡增加。自由采食大鼠的DNA片段百分比具有昼夜变化，当大鼠进食时，其在黑暗时期降低。小肠细胞凋亡的昼夜变化与小肠鸟氨酸脱羧酶活性呈镜像关系。大鼠第三脑室注射2-脱氧-D-葡萄糖可减少肠粘膜细胞凋亡。这种影响，至少部分，通过外侧下丘脑。这些结果提示中枢神经系统可能参与了大鼠肠粘膜细胞凋亡的调控。

项目成果

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S.Ogata、K.Fujimoto、R.Iwakiri、C.Matsunaga、Y.Okawa、T.Koyama、T.Sakai：“聚葡萄糖对肠系膜淋巴瘘大鼠吸收甘油三酯和胆固醇的影响”Pro.Soc.Exp

S.Ogata,et al.: "Effect of polydeytrose on absorption of triglyceride and cholesterol in mesenteric lymph-fistula rats." Proc.Soc.Exp.Biol.Med.215. 53-58 (1997)

S.Ogata 等人：“聚葡萄糖对肠系膜淋巴瘘大鼠吸收甘油三酯和胆固醇的影响”。

T.Noda, R.et al.: "Programmed cell death induced by ischemia-reperfusion in rat intestinal mucosa." Am.J.Physiol.(in press).

T.Noda, R.et al.：“大鼠肠粘膜缺血再灌注诱导的程序性细胞死亡。”

Y.Sakata,et al.: "Role of post-absorptive factors on satiation following a lipid meal in rats." Am.J.Physiol.271. G438-G442 (1996)

Y.Sakata 等人：“吸收后因素对大鼠脂肪餐后饱足感的作用”。

Y.Sakata, et al.: "Role of post-absorptive factors on satiation following a lipid meal in rats." Am.J.Physiol.271. G438-G442 (1996)

Y.Sakata 等人：“吸收后因素对大鼠脂肪餐后饱足感的作用”。