Analysis of liver metastasis mechanism by an antibody to TF antigen

TF抗原抗体分析肝转移机制

• 批准号: 08671495
• 负责人: YASUTOMI Masayuki
• 金额: $ 1.22万
• 依托单位: Kinki University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671495/
• 关键词: neuraminidase; Colon 26; Thomsen-Friedenreich antigen; liver metastasis; Neuraminidase; Colon26細胞; モノクローナルガラクトース抗体; CrypticT抗原; Thomsen-Friedenreich抗原

Thomsen-Friedenreich antigen (TF ; Galbeta1-3GalNAcalpha1) is expressed onmany human carcinomas. Evidence suggests that TF-carrying tumor cells specifically bind asialoglycoprotein receptors on hepatocytes with the consequence of metastasis formation in the liver. We used an animal model for examining the feasibility of preventing metastasis formation byan antibody to TF.Treatment of Colon 26 cells with neuraminidase led to the exposure of TF, and consequently to a higher frequency of liver metastases in syngeneic Balb/c mice. This could be prevented by an antibody to TF(A78-G/A7), but not by a control antibody. The results mayopen up a new strategy for the prophylaxis of metastatic spread to the liver.

Thomsen-Friedenreich 抗原（TF；Galbeta1-3GalNAcalpha1）在许多人类癌症中表达。有证据表明，携带 TF 的肿瘤细胞特异性结合肝细胞上的脱唾液酸糖蛋白受体，从而在肝脏中形成转移。我们使用动物模型来检查通过 TF 抗体预防转移形成的可行性。用神经氨酸酶处理结肠 26 细胞导致 TF 暴露，从而导致同基因 Balb/c 小鼠中肝转移的发生率更高。这可以通过 TF(A78-G/A7) 抗体来预防，但不能通过对照抗体来预防。这些结果可能开辟一种预防转移扩散到肝脏的新策略。

项目成果

Hironori Shigeoka, Uwe Karsten, Kiyotaka Okuno, Masayuki Yasutomi: "Inhibition of liver metastases from neuraminidase-treated colon 26 cells an anti-Thomsen-Friedenreich-specific monoclonal antibody" Tumor Biology. (in press).

Hironori Shigeoka、Uwe Karsten、Kiyotaka Okuno、Masayuki Yasutomi：“抗 Thomsen-Friedenreich 特异性单克隆抗体对经神经氨酸酶处理的结肠 26 细胞的肝转移的抑制”肿瘤生物学。