Investigation for human papillomavirus type-specific cytopathic effect and related epidermoid cysts

人乳头瘤病毒型特异性细胞病变效应及相关表皮样囊肿的研究

• 批准号: 08670975
• 负责人: EGAWA Kiyofumi
• 金额: $ 1.6万
• 依托单位: KUMAMOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670975/
• 关键词: human papillomavirus; type-specific cytopathic effect; intracytoplasmic inclusion body; inclusion wart; epidermoid cyst; eccrine duct; CEA; E4; ヒトパピローマウイルス; 封入体疣贅

1.In our previous studies, it was revealed that a family of HPVs, HPV4,60 and 65, is associated with not only a specific histologic feature of the homnogeneous intracytoplasmic inclusion bodies but also a characteristic clinical picture of the pigmented warts. In this study we have clearly demonstrated that melanin granules were increased specifically in HPV4/60/65-associated warts, suggesting HPV type-specific pigmentary disorder (Br J Dermatol, 1998). We will attempt immunohistochemical and molecular biological studies to understand the pathomechanism of the HPV type-specific pigmentary disorder in our next year's project.2.HPV 60 is the only virus that has been found in plantar epidermoid cysts. In the present study we have found HPV 57 in a plantar epidermoid cyst which showed a different histological feature from that of HPV 60-associated plantar epidermoid cyst (Br J Dermatol, 1998). We are investigating the relationshop between the epidermoid cysts and HPV 57 in much larger number of plantar epidermoid cysts.Using computor we performed three-dimensional reconstruction analysis for the structure of HPV-associated plantar epidermoid cysts, revealing the fine structure of an epidermoid cyst of eccrine duct origin.

1.在我们以前的研究中，发现HPV家族HPV 4、60和65不仅与细胞浆内包涵体的特异性组织学特征有关，而且与色素疣的特征性临床表现有关。在这项研究中，我们已经清楚地表明，黑色素颗粒在HPV 4/60/65相关疣中特异性增加，提示HPV类型特异性色素沉着疾病（Br J Dermatol，1998）。我们将尝试免疫组化和分子生物学研究，以了解HPV型特异性色素沉着疾病的病理机制，在我们明年的项目。2. HPV 60是唯一的病毒，已被发现在足底表皮样囊肿。在本研究中，我们在足底表皮样囊肿中发现了HPV 57，其组织学特征与HPV 60相关的足底表皮样囊肿不同（Br J Dermatol，1998）。我们在大量的足底表皮样囊肿中研究了表皮样囊肿与HPV 57的关系，并利用计算机对HPV相关的足底表皮样囊肿的结构进行了三维重建分析，揭示了一个起源于小汗腺管的表皮样囊肿的精细结构。

项目成果

Astori G,Lavergne D,Benton C,Hoeckmayr B,Egawa K,Garbe C,de Villiers E-M.: "The papillomaviruses found in benign and malignant cutaneous lesions are present in normal skin." Journal of Investyigative Dermatology. (in press).

Astori G、Lavergne D、Benton C、Hoeckmayr B、Egawa K、Garbe C、de Villiers E-M.：“在良性和恶性皮肤病变中发现的乳头瘤病毒也存在于正常皮肤中。”

Aston G.: "The papillomavirus found in benign and malignant cutanecus lesions are present in normal skin." Journal of Investigative Dermatology. (in press).

Aston G.：“在良性和恶性皮肤病变中发现的乳头瘤病毒也存在于正常皮肤中。”

Yumi Honda: "Hair cycle-dependent expression of a nonspecific cross reacting antigen (NCA)-50/90-like molecule on follicular keratinocytes" Arch Derm Res. 289. 457-465 (1997)

Yumi Honda：“毛囊角质形成细胞上非特异性交叉反应抗原 (NCA)-50/90 样分子的毛发周期依赖性表达”Arch Derm Res。

Honda Y.: "Hair cycle-dependent expression of a nonspecific cross reacting antigen (NCA)-50/90-like molecule on follicular keratinocytes" Archives of Dermatological Reseach. 289. 457-465 (1997)

Honda Y.：“毛囊角质形成细胞上非特异性交叉反应抗原 (NCA)-50/90 样分子的毛发周期依赖性表达”皮肤病学研究档案。

Kiyofumi Egawa: "Pigmented viral warts:a clinical and histopathological study including human papillomavirus subtype." Br J Dermatol. 138(in press). (1998)

Kiyofumi Ekawa：“色素病毒疣：一项包括人乳头瘤病毒亚型的临床和组织病理学研究。”