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Mollecular histopathological study for human papillomavirus type-pecific cytopathic effect

人乳头瘤病毒型特异性细胞病变效应的分子组织病理学研究

基本信息

批准号：
18591254
负责人：
EGAWA Kiyofumi
金额：
$ 2.48万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591254/
关键词：
Virus Cancer Cell-Tissue HPV Stem cell

项目摘要

Although it is now accepted that there are correlations between human papillomavirus (HPV) types and specific cytopathic changes (HPV-type-specific cytopathic effect: CPE), the true mechanism of the CPE is still unknown. There are as yet little evidences for the target cells of HPVs, either. In a series of previous studies of our own, we found out HPV 60, 63, 65, 88 and 95 in new clinical types of viral warts, in which particular intracytoplasmic inclusion bodies were seen type-specifically. It is now accepted that genus gamma-papillomavirus consists of HPV 4, 48, 50, 60, 65, 88 and 95, while genus mu HPV 1 and 63. A characteristic of these genera is producing various intracytoplasmic inclusion bodies (ICBs) in genotype-specific manner. In the present study, it has been revealed that the heterogeneous ICBs, and their association with specific types of HPV, will provide a useful model for studying the interaction between keratinocytes and HPVs, especially in the functional aspect of E4 gene expression. Aside from the HPV type-specific ICBs, HPV 4/60/65-associated pigmented warts implicate HPV type-specific activation of melanogenesis HPV 60 provides a new clinical entity of HPV-associated epidermoid cysts; a reported double infection with HPV 1 and HPV 63 within a single cell with only HPV 63-associated ICB poses an important problem of a possible interference between the viruses: HPV 63-induced punctuate wart has recently been described as an excellent clue to epidermal stem cells whose exact localization is not yet clear; and HPV 1-induced hypertrichosis observed in a patient suggests that HPV infection not only induce visible lesions but also activate its neighboring epithelium. On the basis of our results, we suggest that the genus gamma- and mu-papiUomaviruses pose important problems to be resolved in virology and human pathology

虽然现在人们认为人乳头瘤病毒（HPV）类型与特异性细胞病变（HPV-type-specific cytopathic effect: CPE）之间存在相关性，但CPE的真正机制仍不清楚。到目前为止，关于hpv的靶细胞的证据也很少。在我们自己之前的一系列研究中，我们在新的临床类型的病毒性疣中发现了HPV 60、63、65、88和95，其中特定的胞浆内包涵体是特定类型的。目前公认的伽玛乳头瘤病毒属包括HPV 4、48、50、60、65、88和95，而属则包括HPV 1和63。这些属的一个特点是以基因型特异性的方式产生各种胞浆内包涵体（ICBs）。本研究揭示了异质ICBs及其与特定类型HPV的关联，将为研究角质形成细胞与HPV之间的相互作用，特别是在E4基因表达的功能方面提供一个有用的模型。除了HPV类型特异性的ICBs， HPV 4/60/65相关的色素疣涉及HPV类型特异性的黑色素生成激活HPV 60提供了HPV相关的表皮样囊肿的新的临床实体；据报道，单个细胞内HPV 1和HPV 63的双重感染仅与HPV 63相关的ICB提出了病毒之间可能干扰的重要问题：HPV 63诱导的点状疣最近被描述为表皮干细胞的极好线索，其确切定位尚不清楚；在患者中观察到的HPV - 1诱导的多毛症表明HPV感染不仅诱导可见的病变，而且还激活其邻近的上皮。基于我们的研究结果，我们认为伽玛乳头瘤病毒和乳头瘤病毒属在病毒学和人类病理学中提出了需要解决的重要问题