Mollecular histopathological study for human papillomavirus type-pecific cytopathic effect

点击翻译按钮获取中文摘要

Although it is now accepted that there are correlations between human papillomavirus (HPV) types and specific cytopathic changes (HPV-type-specific cytopathic effect: CPE), the true mechanism of the CPE is still unknown. There are as yet little evidences for the target cells of HPVs, either. In a series of previous studies of our own, we found out HPV 60, 63, 65, 88 and 95 in new clinical types of viral warts, in which particular intracytoplasmic inclusion bodies were seen type-specifically. It is now accepted that genus gamma-papillomavirus consists of HPV 4, 48, 50, 60, 65, 88 and 95, while genus mu HPV 1 and 63. A characteristic of these genera is producing various intracytoplasmic inclusion bodies (ICBs) in genotype-specific manner. In the present study, it has been revealed that the heterogeneous ICBs, and their association with specific types of HPV, will provide a useful model for studying the interaction between keratinocytes and HPVs, especially in the functional aspect of E4 gene expression. Aside from the HPV type-specific ICBs, HPV 4/60/65-associated pigmented warts implicate HPV type-specific activation of melanogenesis HPV 60 provides a new clinical entity of HPV-associated epidermoid cysts; a reported double infection with HPV 1 and HPV 63 within a single cell with only HPV 63-associated ICB poses an important problem of a possible interference between the viruses: HPV 63-induced punctuate wart has recently been described as an excellent clue to epidermal stem cells whose exact localization is not yet clear; and HPV 1-induced hypertrichosis observed in a patient suggests that HPV infection not only induce visible lesions but also activate its neighboring epithelium. On the basis of our results, we suggest that the genus gamma- and mu-papiUomaviruses pose important problems to be resolved in virology and human pathology