Human papillomavirus type-specific cytopathogenic effect

人乳头瘤病毒类型特异性致细胞病变作用

• 批准号: 05670734
• 负责人: EGAWA Kiyofumi
• 金额: $ 1.41万
• 依托单位: KUMAMOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670734/
• 关键词: HPV; Cytopathogenic effect; Intracytoplasmic inclusion body; HPV 60; HPV 63; HPV 65; Epidermoid cyst; Eccrine duct; CPE; Inclusion wart; HPV60; HPV63; HPV65; ヒトパピローマウイルス; 型特異的細胞変性効果; 細胞質内封入体; 封入体疣贅; 表皮様嚢腫; エックリン汗管; CEA; 封入体疾質

(1) We revealed that two new types of intracytoplasmic inclusion bodies (ICBs) were associated with distinct clinical features, and the presence of DNA of distinct types of human papillomaviruses. HPV 1 DNA sequences were detected in all the lesions with well-known granular ICBs. Punctate keratotic lesions with filamentous ICBs were associated with HPV 63, which was newly cloned from such a lesion. Pigmented warts with homogeneous ICBs contained one of the related HPVs, i.e., HPV 4, HPV 60 or a novel type of HPV,HPV 65. Based on these associations, a classification of inclusion warts is proposed.(2) Although epidermoid cysts of the palms and soles have long been assumed to develop following implantation of an epidermal fragments as a result of a penetrating injury, the pathomechanism is still controversial. Clinical, histological, immunohistochemical and molesular biological studies were performed on 119 epidermoid cysts of palmoplantar location, inorder to examine the role of the eccrine ducts, and human papillomavirus (HPV) , in the pathogenesis of this disorder.Either ductal structures of carcinoembryonic antigen expression were noted in a total of 73 cases (61%). Papillomavirus common antigens were detected in 36 cases (30%) showing one or more of the three distinct histological features, i.e., intracytoplasmic inclusion bodies (ICBs) , vacuolar structres and parakeratosis in the cyst cavity. On the basis of our results, we propose that epidermoid cysts in the palmoplantar regions may develop from eccrine ducts, and that HPV may play a role in their pathogenesis.

(1)我们发现，两种新类型的胞浆内包涵体（ICBs）与不同的临床特征，和不同类型的人乳头瘤病毒的DNA的存在。所有颗粒状ICB病灶均检出HPV 1 DNA序列。点状角化病变与丝状ICBs与HPV 63，这是新克隆从这样的病变。具有同质ICB的色素性疣含有一种相关HPV，即，HPV 4、HPV 60或新型HPV 65。基于这些协会，列入疣的分类提出。(2)虽然掌跖表皮样囊肿长期以来被认为是由于穿透伤导致表皮碎片植入后发生的，但其病理机制仍有争议。本文对119例掌跖部表皮样囊肿进行了临床、组织学、免疫组化及分子生物学研究，以探讨小汗腺导管及人乳头瘤病毒（HPV）在该病发病中的作用。在36例（30%）病例中检测到乳头瘤病毒共同抗原，显示三种不同组织学特征中的一种或多种，即，胞浆内包涵体、空泡状结构和囊腔内角化不全。根据我们的研究结果，我们认为掌跖区的表皮样囊肿可能是由小汗腺导管引起的，HPV可能在其发病机制中起作用。

项目成果

Egawa K.: "Two novel types of human papillomavirus, HPV63 and HPV65: Comparisons of their clinical and histoligical features and DNA sequences to other HPV types." Virology. 194. 789-799 (1993)

Ekawa K.：“两种新型人乳头瘤病毒，HPV63 和 HPV65：它们的临床和组织学特征以及 DNA 序列与其他 HPV 类型的比较。”

EgawaK,Delius H,Matsukura T,Kawashima M,de Villiers EM.: "Two novel types of human papillomavirus, HPV 63 and HPV 65 : Comparisons of their clinical and histological features and DNA sequences to other HPV types." Virology. 194. 789-799 (1993)

EkawaK、Delius H、Matsukura T、Kawashima M、de Villiers EM.：“两种新型人乳头瘤病毒，HPV 63 和 HPV 65：其临床和组织学特征以及 DNA 序列与其他 HPV 类型的比较。”

KIYOFUMI EGAWA: "Multiple plantar epidermoid cysts harboring carcinoembryonic and human papillomavirus DNA sequences" Journal of American Academy of Dermatology. 30. 494-496 (1994)

KIYOFUMI EGAWA：“含有癌胚和人乳头瘤病毒 DNA 序列的多个足底表皮样囊肿”美国皮肤病学会杂志。

KIYOFUMI EGAWA: "Detection of human papillomaviruses and eccrine ducts in Dalmoplantar epidermoid cysts" British Journal of Dermatology. 132(in press). (1995)

KIYOFUMI EGAWA：“足底表皮样囊肿中人乳头瘤病毒和小汗腺管的检测”英国皮肤病学杂志。

Honda Y.: "Sweat duct milia-immunohistochemical and three-dimensional reconstruction analysis of structure." Archives of Dermatologica Research. 288(in press). (1996)

Honda Y.：“汗管粟粒症-免疫组织化学和结构三维重建分析。”