Anapylactic reaction and cytokine expression in gut form mice oral challnged with ovalbumin

口服卵清蛋白小鼠肠道的过敏反应和细胞因子表达

• 批准号: 08670974
• 负责人: TANAKA Yoichi
• 金额: $ 1.41万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670974/
• 关键词: gut; food allergy; eosinophil; IL-5; chemokine; ovalbumin; eosonighil; in situ hybridization

We got the results described below.1. Balb/c mice were intraperitonealy immunized with ovalbumin (OVA) and alum, and high anti-OVA IgE titers were observed by passive cutaneous anapylaxis (pCA).2. Antigen-specific significanti eosinophil infiltrations were observed in lamina propria of gut by oral challenge with OVA,and peaked at 6h.3. The eosinophil infiltrations were significantly inhibited by preadministration with monoclonal anti-mouse IL-5 antibody.4. IL-5 mRNA positive cells were demonstrated in lamina propria by in situ hybridization.5. RANTES mRNA expression was observed in gut by reverse transcription polymerase chain reaction (RT-PCR), but it was not non-specific. Eotaxin mRNA was not detected by the method.6. Transient mRNA expressions of ICAM-1 and VCAM-1 (6h after oral challenge) were observed in gut.These results suggested important role of IL-5 in eosinophil infiltration into gut.

我们得到了下面描述的结果。用卵清蛋白（OVA）和明矾腹腔免疫Balb/c小鼠，通过被动皮肤过敏反应（pCA）观察到较高的抗OVA IgE滴度.免疫组化结果显示，卵清蛋白可诱导肠黏膜固有层嗜酸性粒细胞浸润，6 h达高峰。预先给予抗小鼠IL-5单克隆抗体可显著抑制嗜酸性粒细胞浸润.原位杂交显示固有层内有IL-5 mRNA阳性细胞.逆转录聚合酶链反应（RT-PCR）检测肠道RANTES mRNA表达，但其表达并非非特异性。该方法未检测到嗜酸粒细胞趋化因子mRNA。6.灌胃后6 h，肠组织中可见ICAM-1和VCAM-1 mRNA的瞬时表达，提示IL-5在嗜酸性粒细胞向肠组织浸润中起重要作用。

项目成果

Miguel A Vinuesa: "In situ expression of interleukin-4, -5 and -6 in Peyer's patch from ovalbumin-sensitized BALB/c mice after oral challerge" Allergology International. 46. 243-247 (1997)

Miguel A Vinuesa：“口服攻击后卵清蛋白致敏的 BALB/c 小鼠派尔氏斑中白细胞介素 4、-5 和 -6 的原位表达”，Allergology International。