Cytokine gene expression in atopic dermatits

特应性皮炎中细胞因子基因的表达

• 批准号: 06670870
• 负责人: TANAKA Yoichi
• 金额: $ 1.41万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670870/
• 关键词: atopic dermatitis; Th1; Th2; IL-4; IFN-gamma; in situ hybridization; Th1; Th2; ダニパッチテスト; エレ-4

To date, a number of findings suggesting a predominance of type 2 helper T cell (Th2) have been reported in atopic dermatitis (AD). On the other hand, a predominance of interferon-gamma (IFN-gamma) messenger RNA (mRNA) expression was recently demonstrated by reverse transcription polymerase chain reaction (RT-PCR) in the lesions of AD.To address the controversial issue, we investigated both transcription and translation of interleukin-4 (IL-4) and IFN-gamma in the lesions of five AD patients by in situ hybridization (ISH) and immunostaining. In mRNA and protein level, coexpression of IL-4 and IFN-gamma was observed. The number of IFN-gamma mRNA-positive cells (14.0<plus-minus>6.7) was significantly higher than that of IL-4 mRNA-positive cells (2.4<plus-minus>1.1) (p=0.04). The number of positive staining cells of IFN-gamma (6.3<plus-minus>2.8) were also significantly higher than that of IL-4 (1.8<plus-minus>1.2) (p=0.04). However, the number of IL-4 mRNA-positive cells tended to correlate with the AD severity (r=0.76, p=0.13), suggesting an important role of IL-4 in the pathogenesi

迄今为止，许多研究结果表明，2型辅助性T细胞（Th 2）占主导地位的特应性皮炎（AD）已被报道。另一方面，干扰素-γ（IFN-γ）的信使RNA（mRNA）的表达优势最近被证明在AD病变的逆转录聚合酶链反应（RT-PCR）。为了解决有争议的问题，我们研究了转录和翻译的白细胞介素-4（IL-4）和IFN-γ在5例AD患者的病变原位杂交（ISH）和免疫染色。在mRNA和蛋白水平上，观察到IL-4和IFN-γ的共表达。IFN-γ mRNA阳性细胞数（14.0 ± <plus-minus>6.7）显著高于IL-4 mRNA阳性细胞数（2.4 <plus-minus>± 1.1）（p=0.04）。IFN-γ的阳性染色细胞数（6.3 ± <plus-minus>2.8）也显著高于IL-4的阳性染色细胞数（1.8 <plus-minus>± 1.2）（p=0.04）。然而，IL-4 mRNA阳性细胞的数量与AD的严重程度相关（r=0.76，p=0.13），提示IL-4在AD的发病机制中起重要作用。

项目成果

Tanaka Y,Zhang HM,Bae SJ,Hakugawa J,Takenaka M,Anan S and Katayama I: "Coexpression of interleukin-4 and interferon-gamma messenger RNA in lesional skin of atopic dermatitis" (in preparation).

Tanaka Y、Zhang HM、Bae SJ、Hakukawa J、Takenaka M、Anan S 和 Katayama I：“特应性皮炎病变皮肤中白细胞介素 4 和干扰素 γ 信使 RNA 的共表达”（准备中）。

Tanaka Y et al: "Coexpression of interleukin-4 and interferon-γ messenger RNA in lesional skin of atopic dermatitis" (in preparation).

Tanaka Y 等人：“特应性皮炎病变皮肤中白细胞介素 4 和干扰素 γ 信使 RNA 的共表达”（准备中）。