权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

MORPHOLOGICAL AND MOLECULAR BIOLOGICAL STUDIES FOR HTLV-I INFECTION

HTLV-I 感染的形态学和分子生物学研究

基本信息

批准号：
08670976
负责人：
SETOYAMA Mitsuru
金额：
$ 1.02万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670976/
关键词：
ATLL HTLV-I in situ hybridization PCR- in situ hybridization tropism HTLV-1 Ecerine Sweat gland PCR in situ hybridization In situ hybridization HTL

项目摘要

Human T-cell lymphotropic virus type I (HTLV-I) is an RNA virus which is considered to be etiologic factor of neoplastic and non-neoplastic disorders in several organs as well as peripheral blood. Recently, pathological relationship between HTLV-I and HTLV-I-related disorders including adult T-cell leukemia/lymphoma (ATLL) and. HAM/TSP, have been clarified by using methods based on molecular biology and immunology. However, distribution and subcellular localization of the virus in lesions remain unclear. Therefore, we aim to investigate a localization of the virus in peripheral blood and the lesional skin from HTLV-I-infected patients by using of in situ hybridization and PCR-in situ hybridization techniques. As results, HTLV-I mRNA were expressed in lymphoid cells or lymphocytes in frozen sections of skin samples from healthy carrier and ATLL patients. There might be no significant correlation between subtype of ATLL and frequency of signals. On the other hand, interestingly, HTLV-I tax sequence was recognized in eccrine sweat gland epithelia in some HTLV-I infected cases by PCR-ISH technique. To the best of our knowledge, there is no report describing HTLV-T infection to sweat gland epithelia. The findings may be important to consider the route of infection and the etiology of non-neoplastic disorder caused by this virus.

人类 T 细胞嗜淋巴细胞病毒 I 型（HTLV-I）是一种 RNA 病毒，被认为是多种器官以及外周血中肿瘤性和非肿瘤性疾病的病因。最近，HTLV-I 和 HTLV-I 相关疾病之间的病理关系，包括成人 T 细胞白血病/淋巴瘤 (ATLL) 和。 HAM/TSP已通过基于分子生物学和免疫学的方法得到澄清。然而，病毒在病变中的分布和亚细胞定位仍不清楚。因此，我们的目的是通过原位杂交和PCR原位杂交技术研究HTLV-I感染患者外周血和皮损皮肤中病毒的定位。结果，HTLV-I mRNA在来自健康携带者和ATLL患者的皮肤样本的淋巴细胞或冷冻切片中的淋巴细胞中表达。 ATLL 亚型和信号频率之间可能没有显着相关性。另一方面，有趣的是，通过PCR-ISH技术，在一些HTLV-I感染病例的小汗腺上皮细胞中识别出了HTLV-Itax序列。据我们所知，尚无关于汗腺上皮细胞HTLV-T感染的报道。这些发现对于考虑这种病毒引起的非肿瘤性疾病的感染途径和病因可能很重要。