权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Immunocytochemical and ultrastructural examination of amyloid plaques in the brains with Alzheimer-type dementia.

阿尔茨海默型痴呆患者大脑中淀粉样斑块的免疫细胞化学和超微结构检查。

基本信息

批准号：
08671119
负责人：
ARIMA Kunimasa
金额：
$ 1.34万
依托单位：
Tokyo Institute of Psychiatry
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671119/
关键词：
Alzhemer's disease Amyloid Astrocyte Electron microscopy Gliosis Paired helical filaments Senile plaque Tau protein

项目摘要

This is a detailed immunohistochemical and ultrastructural examination of "amyloid plaques" in the brains of Alzheimer's disease. The amyloid plaque is a distinctive type of senile palques, in which thin glial bundles penetrate into the mass of amyloid fibrils accompanied with very few degenerating axon terminals, first described by Oyanagi (1990) under the electron microscope in certain cases of Alzheimer's disease.Subjects : Seven clinically and pathologically well-documented Alzheimer's disease cases were examinedResults : 1. Histological studies. Numerous amyloid plaques were found in two cases, in which amyloid plaques tended to occurred in the parietal and temporal cortices and in the floor of the gyrus. Only few amyloid plaques were found in the floor of gyri in the parietal and temporal lobes in three cases. No amyloid plaques were found in two cases.2. Immunohistochemical studies. The amyloid core of the amyloid plaques showed positive immunoreaction with all of the anti-beta- … More amyloid antibodies that recognized amino-acid residues of 1-40,1-42, and 8-17. In terms of molecular spices of Abeta-protein, both of Abeta1-40 and Abeta1-42 were identified. Anti-GFAP antibody stained thin glial bundles that penetrated into the amyloid mass. Anti-tau antibodies visualized only few neuronal processes surrounding the amyloid mass.3. Electron microscopic studies. Astrocytic glial bindles were identified in amyloid plaques. A particular form of astrocytic degeneration called "astrocytic glial tangles" were found in 3 cases that had amyloid plaques. The astrocytic glial tangles consisted of abnormal tubular structures that resembled PHFs and straight tubules of neurofibrillary tangles.4. Immuno-electron microscopy and electron microscopic observation of Gallyas-Braak's silver impregnation sections. Abnormal tubules in the astrocytes showed argyrophilia and anti-tau immunoreactivity.Conclusions : The amyloid plaque was a distinctive type of senile plaques in that the former lacked degenerating axon terminals and that the former had intense astrocytic reaction to the amyloid mass. Association between the occurrence of amyloid plaques and the clinical duration was not found. Less

这是对阿尔茨海默病患者大脑中“淀粉样斑块”的详细免疫组织化学和超微结构检查。淀粉样斑块是一种独特类型的老年斑块，其中薄的神经胶质束渗入淀粉样原纤维团中，并伴有很少的退化轴突末端，首次由 Oyanagi (1990) 在电子显微镜下在某些阿尔茨海默病病例中描述。对象： 7 个临床和病理学上有充分记录的阿尔茨海默病病例检查结果： 1.组织学研究。 2例发现大量淀粉样斑块，其中淀粉样斑块多发生在顶叶皮质、颞叶皮质和脑回底层。 3例患者的顶叶和颞叶回底仅发现少量淀粉样斑块。 2例均未发现淀粉样斑块。2．免疫组织化学研究。淀粉样斑块的淀粉样蛋白核心与所有识别 1-40、1-42 和 8-17 氨基酸残基的抗 β-淀粉样蛋白抗体均显示出阳性免疫反应。在Abeta蛋白的分子香料方面，鉴定出Abeta1-40和Abeta1-42。抗 GFAP 抗体对渗透到淀粉样蛋白块中的细神经胶质束进行染色。抗 tau 抗体仅使淀粉样蛋白块周围的少数神经元过程可视化。3。电子显微镜研究。在淀粉样斑块中鉴定出星形胶质细胞结合。在 3 例具有淀粉样斑块的病例中发现了一种特殊形式的星形细胞变性，称为“星形细胞胶质缠结”。星形胶质细胞缠结由类似PHF的异常管状结构和神经原纤维缠结的直管组成。4． Gallyas-Braak 银浸渍切片的免疫电子显微镜和电子显微镜观察。星形胶质细胞中的异常小管表现出嗜银和抗tau免疫反应性。结论：淀粉样斑是老年斑的一种独特类型，前者缺乏退化的轴突末端，并且前者对淀粉样蛋白块具有强烈的星形胶质细胞反应。未发现淀粉样斑块的发生与临床持续时间之间的关联。较少的