Effects of coordinated stimulations triggered by interictal discharges on kindling-induced epileptogenesis.

人们普遍认为，引燃引起的间歇放电（IIDs）与癫痫患者的IIDs几乎相似。然而，它们的一般特征，即它们是反映兴奋性事件还是抑制性事件，以及它们是如何产生的，仍然是未知的。在以往的研究中，我们将引燃引起的IIDs分为简单A、简单B和复杂三种类型。复杂类型由2 - 3个简单IIDs组成，其频率与点燃过程中行为反应的发展有关。本实验旨在揭示IIDs触发的协同刺激对家兔海马点火诱导的癫痫基因的影响。在17只动物中，10只出现5期惊厥，平均刺激19次（K组），而其余的没有（IK组）。在日常点火刺激之前，在改变刺激参数的同时，采用协调刺激产生后放电，协调刺激是随机驱动（RS）或由I…More ID （TS）触发的微型点火刺激。最终刺激强度（电压*频率*次数）以引火量为基础，将其标准化，称为标准强度指数（SII）。K组平均sii为28(]SY.+-。[)3%（当刺激由简单的A型IIDs触发时：n=7）， 32(]SY.+-。[)4%（简单B: n=16）和39(]SY.+-。[)4%（复数：n=24），而38(]SY.+-。[)随机施加5% （n=79）。IK组为48（±±）。[)5%（简单A: n=11）， 75(]SY.+-。[)4%（简单B: n=4）， 42(]SY.+-。[)3%（复数：n=10）和55(]SY.+-。[)5%（随机n=52）。TS和RS的SII差异为-10 ~ -7%（简单A）， -6 ~ +20%（简单B）和-23 ~ +1%（复杂）。结果表明，单纯B型IIDs对癫痫发作易感性具有抑制作用，单纯A型和复杂IIDs对癫痫发作易感性具有促进作用。少

It is generally accepted that kindling-induced interictal discharges (IIDs) are almost similar to IIDs of epileptic patients. However, their general characteristics, i.e., whether they reflect an excitatory event or an inhibitory one and how they are generated, are still unknown. In previous studies, we classified kindling-induced IIDs into three types : simple A,simple B and complex. The complex type consisted of two-three simple IIDs, and its frequency was associated with the development of behavioral responses during kindling.The present experiment was designed to reveal effects of coordinated stimulations triggered by IIDs on epileptogenes induced by hippocampal kindling of the rabbits. Out of 17 animals, ten developed stage 5 convulsions with a mean of 19 stimulations (K group), whereas the remaining did not (IK group). Before daily kindling stimulation, a coordinated stimulation, which was a miniature type of kindling stimulation either driven at random (RS) or triggered by the I … More ID (TS), was applied to generate an afterdischarge while changing stimulus parameters. The final stimulus intensity (voltage*frequency*no.of train) was standardized based on those of kindling and referred to as a standard intensity index (SII). In K group, averaged SIIs were 28(]SY.+-。[)3% (when the stimuli were triggered by simple A type of IIDs : n=7), 32(]SY.+-。[)4% (simple B : n=16) and 39(]SY.+-。[)4% (complex : n=24), while it was 38(]SY.+-。[)5% when applied at random (n=79). Those of IK group were 48(]SY.+-。[)5% (simple A : n=11), 75(]SY.+-。[)4% (simple B : n=4), 42(]SY.+-。[)3% (complex : n=10) and 55(]SY.+-。[)5% (at random : n=52), respectively. The SII differences between TS and RS were -10 to -7% (simple A), -6 to +20% (simple B) and -23 to +1% (complex). Since negative values indicate weaker stimuli to generate afterdischarges than RS intensity whereas positive values do stronger ones, it is concluded that simple B type of IIDs has suppressive effect on seizure susceptibility while simple A and complex IIDs have facilitatory effect. Less