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The Superoxide-generating System and its Function in Human B Lymphocytes

人B淋巴细胞超氧化物生成系统及其功能

基本信息

批准号：
08671209
负责人：
KOBAYASHI Sonoko
金额：
$ 1.34万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671209/
关键词：
B lymphocytes CGD surface immunoglobulin Ig class switching EBV-BCL superoxide-generating system superoxide CGD(慢性肉芽腫症)surface Lg Lg class switch

项目摘要

We have shown that human peripheral B lymphocytes contain cytochrome b558 (gp91-, and p22-phox) and cytosolic proteins, p47-and p67-phox, all are known to be essential for the superoxide generation. The amounts of these components in B lymphocytes were much lower than than those in neutrophils, which may reflect the weak superoxide-generating activity of B lymphocytes. Mean amounts (n=8) of gp91-, p22-, p47-and p67-phox in peripheral B lymphocytes as calculated from intensity of the blots were 1.1%, 2.6%, 17.9%and3.9%, relative to those in neutrophils on the basis of cell number. Epstein-Barr virus (EBV) -transformed cell lines derived from normal B lymphocytes also possessed cytochrome b558 and two cytosolic proteins.Based on our observation that EBV-tranaformed B cell lines derived from CGD patients did not show the activity, in contrast to those obtained from normal individuals we concluded that generation of the superoxide anion by B cell lines was due to the presence of the same system as that in phagocytes. Based on our observation that EBV-transformed B cell lines derived from CGD patients did not show the activity, in contrast to those obtained from normal individuals.Since cross-linking of surface immunoglobulins activates the superoxide-generating system in B lymphocytes, it is expected that the superoxide anion is released when the cells are exposed to a specific antigen or when the cells interact antigen-dependently with T lymphocytes. In the latter case, cross-linking of HLA-DR on the B lymphocytes may also take place. It is possible, therefore, that generated superoxide anion modulates immune responses either by affecting antigen-processing or by promoting growth of T lymphocytes. Further studies are needed to elucidate its role.

我们已经表明，人外周B淋巴细胞含有细胞色素b558（gp 91-和p22-phox）和胞质蛋白，p47-和p67-phox，所有已知的是必不可少的超氧化物的产生。这些成分在B淋巴细胞中的含量远低于中性粒细胞，这可能反映了B淋巴细胞产生超氧化物的活性较弱。外周B淋巴细胞中gp 91-、p22-、p47-和p67-phox的平均含量（n=8）分别为中性粒细胞的1.1%、2.6%、17.9%和3.9%。正常B淋巴细胞来源的EB病毒（EBV）转化细胞系也具有细胞色素b558和两种胞浆蛋白。与从正常个体中获得的结果相反，我们得出结论，B细胞系产生超氧阴离子是由于存在与吞噬细胞根据我们的观察，来自CGD患者的EBV转化的B细胞系与来自正常个体的细胞系相反，没有显示出活性。由于表面免疫球蛋白的交联激活了B淋巴细胞中的超氧化物生成系统，因此可以预期，当细胞暴露于特定抗原或当细胞与T淋巴细胞抗原依赖性相互作用时，超氧阴离子被释放。在后一种情况下，B淋巴细胞上的HLA-DR的交联也可能发生。因此，产生的超氧阴离子可能通过影响抗原加工或通过促进T淋巴细胞的生长来调节免疫反应。需要进一步的研究来阐明其作用。