Expression of Heme-oxygenase 1 in the neonatal rat brain with hypoxic-ischemic encephalopathy.

缺氧缺血性脑病新生大鼠脑中血红素加氧酶 1 的表达

• 批准号: 08671317
• 负责人: TAKADA Satoshi
• 金额: $ 1.34万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671317/
• 关键词: Heme-oxygenasel; Hypoxic-ischemic encephalopathy; Neonatal rat; Immuno-peroxidase staining; Northern Blotting; Northern Blotting法; 新生児; 低酸素性虚血性脳障害; Heme Oxygenase-1

Heme-oxygenase 1 (HO-1) is a stress protein and a rate-limiting enzyme in heme degradation, generating carbon monoxide and bile pigment. To study the role of HO-1 in neonatal hypoxia-iachemia (HI), 1 week old wats were subjected to left carotid ligation and exposure to 8% O2/92% N2 for 2 hours.1.Expression of HO-1 in the neonatal rat brain after HIImmuno-histochemical staining revealed that HO-1 positive cells were rich in the CA3 region of hippocampus at 6 hours after HI loading. Western blot analysis showed increased HO-1 staining in the ipsilateral region including hipocampus and striatum at 12 hours upto 24hours after HI.The HO-1levels peaked at 12 hours, and were localized perifocally.2. Effect of HI on the expression of HO-1 mRNA in the neonatal rat brain.We studied HO-1 expression in the neonatal rat brain after HI by using Northern blot analysis, and found increased levels in the ischemic region at 6 hours after HI.Though the bilirubin produced by HO-1 may act as an antioxidant in many tissues, it may also be toxic to the neonatal brain.

血红素加氧酶 1 (HO-1) 是一种应激蛋白，也是血红素降解过程中的限速酶，可产生一氧化碳和胆色素。为了研究HO-1在新生大鼠缺氧缺血(HI)中的作用，对1周龄大鼠进行左颈动脉结扎，并置于8%O2/92%N2环境中2小时。 1.HII后新生大鼠脑内HO-1的表达免疫组织化学染色显示，HI负荷后6小时，海马CA3区HO-1阳性细胞丰富。 Western blot分析显示HI后12小时至24小时，同侧海马和纹状体HO-1染色增加。HO-1水平在12小时达到峰值，并集中于灶周。2． HI对新生大鼠脑中HO-1 mRNA表达的影响。我们通过Northern印迹分析研究了HI后新生大鼠脑中HO-1的表达，发现HI后6小时缺血区的水平升高。虽然HO-1产生的胆红素可能在许多组织中充当抗氧化剂，但它也可能对新生大鼠脑有毒。

项目成果

高田哲: "新生児の低酸素性虚血性脳症" Journal of Clinical Rehabilitation. 7. 81-88 (1998)

Satoshi Takada：“新生儿缺氧缺血性脑病”《临床康复杂志》7. 81-88 (1998)。

Wada, H.: "Severe neonatal nemaline myopathy with delayed maturation of muscle." Brain Dev.18. 135-138 (1996)

Wada, H.：“严重的新生儿线状肌病，伴有肌肉成熟延迟。”