The study of intrathymic islet transplantation.

以胰岛异体移植后免疫抑制为目的，研究了以下三个项目；1.胸腺内胰岛异体移植的疗效，2。基因转染到离体胰岛；主要组织相容性复合体在猪胰岛移植中的作用。1.采用同种异体胰岛移植模型，移植到胸腺的胰岛在单一抗淋巴细胞球蛋白作用下存活超过100天。相比之下，在肾包膜下移植的胰岛存活31.5天（中位生存期）。此外，胸腺内同种异体胰岛移植诱导供者特异性耐受性通过体外试验（MLR）评估。2.采用脂质体法将pZeoSV2/lacZ载体转染大鼠胰岛，经X-gal染色，转染率达90%以上，静孵育检测其内分泌功能良好。3.采用MHC （SLA）匹配的NIH小型猪，进行无免疫抑制的胰岛同种异体移植。受体猪2周以上血糖正常。提示MHC在同种异体胰岛移植中的重要作用。结果表明，多种途径均可实现同种异体胰岛移植，而无需持续的免疫抑制。

For the purpose of immunosuppression after pancreatic islet alloranft, the following three projects were studied ; 1.Efficacy of intrathymic islet alloranft, 2.Gene transfection into isolated islets, 3.The role of Major histocompatibility complex in porcine islet allograft. 1.Using rat islet allograft model, the islets transplanted into the thymus survived more than 100 days with a single antilymphocyte gloublin. In contrast, the islets transplanted beneith the kidney capsel survived 31.5 days (median survival). Furthermore, intrathymic islet allograft induced the donor-specific torelance evaluated by in vitro assay (MLR). 2.The islets isolated from Rat pancreata were transfected with pZeoSV2/lacZ Vector using a liposomal method Transfer rates evaluated by X-gal staining showed over 90% with good endocrine function assayd by static incubation. 3.Using a MHC (SLA) matched NIH miniature pigs, the islet allograft without immunosuppression was perfomed. The recipient pigs showed normoglycemia more than two weeks. This result suggested the important role of MHC on islet allograft. These results demonstrated that various approach might realize the islet allograft wothout continuous immnosuppression.