The study of intrathymic islet transplantation.

胸腺内胰岛移植的研究。

• 批准号: 08671334
• 负责人: KENMOCHI Takashi
• 金额: $ 1.47万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671334/
• 关键词: Islet transplantation; Intrathymic transplantation; Anti lymphocyte gloublin; Gene transfection; Major histocompatibility complex; Anti Lymphocyte gloublin; Major histocompatibility compley; 免疫寛容; ラ島分離

For the purpose of immunosuppression after pancreatic islet alloranft, the following three projects were studied ; 1.Efficacy of intrathymic islet alloranft, 2.Gene transfection into isolated islets, 3.The role of Major histocompatibility complex in porcine islet allograft. 1.Using rat islet allograft model, the islets transplanted into the thymus survived more than 100 days with a single antilymphocyte gloublin. In contrast, the islets transplanted beneith the kidney capsel survived 31.5 days (median survival). Furthermore, intrathymic islet allograft induced the donor-specific torelance evaluated by in vitro assay (MLR). 2.The islets isolated from Rat pancreata were transfected with pZeoSV2/lacZ Vector using a liposomal method Transfer rates evaluated by X-gal staining showed over 90% with good endocrine function assayd by static incubation. 3.Using a MHC (SLA) matched NIH miniature pigs, the islet allograft without immunosuppression was perfomed. The recipient pigs showed normoglycemia more than two weeks. This result suggested the important role of MHC on islet allograft. These results demonstrated that various approach might realize the islet allograft wothout continuous immnosuppression.

以胰岛异体移植后的免疫抑制为目的，进行了以下三个项目的研究： 1.胸腺内同种异体胰岛的功效，2.基因转染到分离的胰岛中，3.主要组织相容性复合物在猪同种异体胰岛移植中的作用。 1.采用大鼠胰岛同种异体移植模型，移植到胸腺的胰岛在单一抗淋巴细胞球蛋白的作用下存活超过100天。相比之下，移植到肾囊下方的胰岛存活了 31.5 天（中位存活期）。此外，胸腺内胰岛同种异体移植物诱导了通过体外测定（MLR）评估的供体特异性耐受性。 2.采用脂质体法转染大鼠胰腺分离胰岛，X-gal染色检测转移率达90%以上，静态孵育检测内分泌功能良好。 3.使用MHC（SLA）匹配的NIH小型猪，进行无免疫抑制的同种异体胰岛移植。受体猪在两周多的时间内表现出正常血糖。这一结果表明MHC对同种异体胰岛移植物的重要作用。这些结果表明，多种方法可以实现无需持续免疫抑制的同种异体胰岛移植。

项目成果

Kenmochi T.et al.: "Allogenic islet transplantation without immunosuppression in swine leukocyte antigen(MHC)-matched miniature swine" Transplantation Proceeding. 28. 1851-1852 (1996)

Kenmochi T.等人：“在猪白细胞抗原（MHC）匹配的小型猪中进行无免疫抑制的同种异体胰岛移植”移植程序。

Kenmochi T.et al: "Successfal gene transter.to muline Pancreatic islets with Polymine transfection reatents" Transplantation Proceeding. (in Phess).

Kenmochi T.等人：“使用多胺转染试剂成功将基因转移至鼠胰岛”移植程序。

Kenmochi T.et al.: "Successsful gene transfer to murine pancreatic islets with polymine transfection reagents" Transplantation Proceeding. (in press).

Kenmochi T.等人：“使用多胺转染试剂成功将基因转移至小鼠胰岛”移植论文集。

Kenmochi T.et al: "Allogenic islet transplantation without immunosuppression in swine leukocyte antigen (MHC) -matched miniature swine." Transplantation Proceeding. 28. 1851-1852 (1996)

Kenmochi T.等人：“在猪白细胞抗原（MHC）匹配的小型猪中进行无免疫抑制的同种异体胰岛移植。”

T.Kenmochi: "Allogenic islet transplantation without Immnosuppression in Swine Leukocyte Antigen (MHC)-matched mimatnre Swine" Transplantation Proceedings. 28(3). 1851-1852 (1996)

T.Kenmochi：“猪白细胞抗原 (MHC) 匹配的 mimatnre 猪中无免疫抑制的同种异体胰岛移植”移植论文集。