Tolerance induction of pancreatic allograft by intrathymic islet transplantation.

通过胸腺内胰岛移植诱导胰腺同种异体移植物的耐受性。

• 批准号: 10671097
• 负责人: KENMOCHI Takashi
• 金额: $ 2.05万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671097/
• 关键词: Islet transplantation; Pancreas transplantation; Tolerance induction; Intrathymic transplantation; Islet isolation; non-heart beating donor; VEGF

1. Tolerance induction of pancreatic allograft by intrathymic islet transplantation : F344 rat islets were transplanted into the thymus of diabeetic WKAH rat with single injection of ALS. Three weeks after intrathymic transplantation, pancreatic graft of F344 rat was transplanted into WKAH rat. Pancreatig graft survival was sfive days (Median survival), which did not show the prolongation as compared to control (6 days).2. Development of Chiba Univ. Islet Preparation Method : We designed a new islet preparation system using mechanical chopper, automated 2-step digestion unit, HES purification with COBE2991 cell professor. Using Beagle dogs, significant improvement of islet yield, purity, insulin release were obtained with our new method as compared to the conventional method.3. Islet isolation from non-heart beating donor : Using Beagle dogs, pancreas were harvested with 30 minutes' warm ischemia with or without in situ machine wash out. High quality islet were isolated when using in situ machine wash out.

1.胸腺内胰岛移植诱导同种异体胰腺移植免疫耐受：将F344大鼠胰岛移植到糖尿病WKAH大鼠胸腺内，一次性注射ALS。胸腺内移植3周后，将F344大鼠胰腺移植到WKAH大鼠体内。胰腺移植物存活时间为5天（中位存活时间），与对照组（6天）相比没有延长.千叶大学胰岛制备方法的开发：我们设计了一种新的胰岛制备系统，采用机械切碎机，自动化两步消化装置，用COBE 2991细胞教授仪纯化HES。以Beagle犬为实验动物，与传统方法相比，新方法可显著提高胰岛产量、纯度和胰岛素释放.从非心脏跳动供体分离胰岛：使用比格犬，在有或没有原位机器冲洗的情况下，用30分钟的热缺血收获胰腺。采用原位机械洗脱法分离出高质量胰岛。

项目成果

剣持敬他: "膵島分離における消化組織保存の重要性"低温医学. 24(1). 36-40 (1998)

Takashi Kenmochi 等人：“在胰岛分离中保存消化组织的重要性”Cryogenic Medicine 24(1) (1998)。

Kenmochi T. et al.: "Lap-1 cold Pregervation solution for isolation of high-quality human pancreatic islets"Pancreas. 17(4). 367-377 (1998)

Kenmochi T. 等人：“用于分离高质量人胰岛的 Lap-1 冷预保存溶液”胰腺。

Kenmochi T. et al.: "Successful gene transfer into murine pancreatic islets using polyamine transfection reagents"Transplantation Proceedings.. 30. 470-472 (1998)

Kenmochi T. 等人：“使用多胺转染试剂将基因成功转移至鼠胰岛”移植论文集.. 30. 470-472 (1998)

Kenmochi T, et al.: "Develpoment of fully automated islet digestion system"Transplantation Proceedings. (in press).

Kenmochi T 等人：“全自动胰岛消化系统的开发”移植论文集。

Kenmochi T, et al.: "Successful gene transfer into murine pancreatic islets using polyamine transfection reagents."Transplantation Proceedings. 30. 470-472 (1998)

Kenmochi T 等人：“使用多胺转染试剂成功地将基因转移到小鼠胰岛中。”移植论文集。