Tolerance induction of pancreatic allograft by intrathymic islet transplantation.

1. 胸腺内胰岛移植诱导同种异体胰腺移植耐受：单次注射ALS将F344个大鼠胰岛移植至糖尿病WKAH大鼠胸腺。胸腺内移植3周后，将F344大鼠胰腺移植物移植到WKAH大鼠体内。胰腺移植生存期为5天（中位生存期），与对照组（6天）相比，没有显示出延长。千叶大学胰岛制备方法的发展：我们设计了一种新的胰岛制备系统，采用机械斩刀、自动二步消化装置、HES纯化COBE2991细胞教授。以Beagle犬为对象，与传统方法相比，新方法可显著提高胰岛产量、纯度和胰岛素释放量。非心脏供体胰岛分离：用Beagle犬，取胰腺，热缺血30分钟，就地机洗或不机洗。采用原位机洗法分离出优质胰岛。

1. Tolerance induction of pancreatic allograft by intrathymic islet transplantation : F344 rat islets were transplanted into the thymus of diabeetic WKAH rat with single injection of ALS. Three weeks after intrathymic transplantation, pancreatic graft of F344 rat was transplanted into WKAH rat. Pancreatig graft survival was sfive days (Median survival), which did not show the prolongation as compared to control (6 days).2. Development of Chiba Univ. Islet Preparation Method : We designed a new islet preparation system using mechanical chopper, automated 2-step digestion unit, HES purification with COBE2991 cell professor. Using Beagle dogs, significant improvement of islet yield, purity, insulin release were obtained with our new method as compared to the conventional method.3. Islet isolation from non-heart beating donor : Using Beagle dogs, pancreas were harvested with 30 minutes' warm ischemia with or without in situ machine wash out. High quality islet were isolated when using in situ machine wash out.