Bio-artificial islet transplantation using self chondrocytes.

实验1。羟乙基淀粉（HES）净化胰岛的效果。与广泛使用的Euro-Ficoll溶液相比，使用HES-Collins溶液可显著提高Beagle胰岛的产量、纯度和活力。临床胰岛移植推荐使用HES-Collins溶液进行胰岛净化。实验2。大鼠胰岛与异体软骨细胞的共培养。将DA大鼠胰岛与Lewis大鼠软骨细胞共培养。培养10天后，胰岛被软骨细胞包围，并维持胰岛素向培养基中的释放。软骨细胞被认为是生物基质胰岛移植的新材料。实验3。基因转移到犬胰岛。采用1)基因枪、2)电分离、3)脂肪转染的方法将PCMS-EGFP转移至小猎犬胰岛。脂肪抽吸技术操作简单，对胰岛的危害小。此外，脂肪转染技术能很好地保留胰岛素的释放。

Experiment 1. Effectiveness of hydroxyethyl starch (HES) on purification of pancreatic islets. The yield, purity, and viability of islets isolated from Beagle pancreata were significantly improved by the use of HES-Collins solution as compared to widely used Euro-Ficoll solution. Islet purification using HES-Collins solution might be recommended for clinical islet transplantation. Experiment 2. Co-culture of rat islets with allogenic chondrocvtes. Islets isolated from DA rat were co-cultured with the chondrocytes isolated from Lewis rat. The islets were surrounded with chondrocytes at 10 days culture and insulin release into media was maintained. Chondrocyte was suggested to become a new material for Bio matrix islet graft. Experiment 3. Gene transfer into canine islets. PCMS-EGFP was transferred into the islats from Beagle pancreata using 1) Gene gun, 2) Electroporarion, and 3) Lipofection. Lipofection technique was simple and less harmful for the isles. Moreover, insulin release was well preserved with Lipofection technique.