Bio-artificial islet transplantation using self chondrocytes.

使用自体软骨细胞进行生物人工胰岛移植。

• 批准号: 12470235
• 负责人: KENMOCHI Takashi
• 金额: $ 7.49万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470235/
• 关键词: islet transplantation; islet isolation; Chondrocytes; Gene transfer; hydroxyethyl starch; 凍結保存; 遺伝子銃

Experiment 1. Effectiveness of hydroxyethyl starch (HES) on purification of pancreatic islets. The yield, purity, and viability of islets isolated from Beagle pancreata were significantly improved by the use of HES-Collins solution as compared to widely used Euro-Ficoll solution. Islet purification using HES-Collins solution might be recommended for clinical islet transplantation. Experiment 2. Co-culture of rat islets with allogenic chondrocvtes. Islets isolated from DA rat were co-cultured with the chondrocytes isolated from Lewis rat. The islets were surrounded with chondrocytes at 10 days culture and insulin release into media was maintained. Chondrocyte was suggested to become a new material for Bio matrix islet graft. Experiment 3. Gene transfer into canine islets. PCMS-EGFP was transferred into the islats from Beagle pancreata using 1) Gene gun, 2) Electroporarion, and 3) Lipofection. Lipofection technique was simple and less harmful for the isles. Moreover, insulin release was well preserved with Lipofection technique.

实验1.羟乙基淀粉(HES)纯化胰岛的效果。与广泛使用的Euro-Ficoll液相比，HES-Collins液显著提高了Beagle胰腺分离胰岛的产量、纯度和活力。临床胰岛移植可推荐使用HES-Collins液进行胰岛纯化。实验2.大鼠胰岛与同种异体软骨细胞共培养。从DA大鼠分离的胰岛与Lewis大鼠分离的软骨细胞共培养。培养10d时，胰岛周围有软骨细胞包围，维持胰岛素向培养液中的释放。软骨细胞有望成为生物基质胰岛移植的新材料。实验3.将基因转移到犬胰岛。用基因枪、电穿孔和脂质体将PCMS-EGFP基因导入Beagle胰腺的胰岛。脂质体介导法操作简单，对胰岛损伤较小。此外，脂质体技术能很好地保存胰岛素的释放。

项目成果

Kenmochi T, et al.: "Improved quality and yield of islets isolated from human pancreas using a two-step digestion method"Pancreas. 20. 184-190 (2000)

Kenmochi T 等人：“使用两步消化方法提高了从人胰腺中分离出的胰岛的质量和产量”胰腺。

Maruyama M, et al.: "Current progress and perspectives in immunoisolated iset transplantation"J Hepatobiliary Pancrent Surg. 7. 347-357 (2000)

Maruyama M 等人：“免疫隔离 iset 移植的当前进展和前景”J Hepatobiliary Pancrent Surg。

坂本 薫 他: "ミニブタを用いたランゲルハンス氏島移植"今日の移植. 14(3). 243-248 (2001)

Kaoru Sakamoto 等人：“使用小型猪的 Langerhans Ujijima 移植”，《今日移植》14(3) (2001)。

和田佑一: "膝関節軟骨損傷に対する自家培養軟骨細胞移植の経験"BIO Clinica. 15. 1140-1143 (2000)

Yuichi Wada：“自体培养软骨细胞移植治疗膝关节软骨损伤的经验”BIO Clinica 15. 1140-1143 (2000)。

Kenmochi T et al.: "Purification of pancreatic islets using hydroxyethyl starch(HES)-Collins solution"Transplantation Proceedings. 33. 670-671 (2001)

Kenmochi T 等人：“使用羟乙基淀粉 (HES)-柯林斯溶液纯化胰岛”移植论文集。