SDUDIES OF HIGH MOLECULAR SUBSTANCE- SOD CONJUGATE ON PREVENSION OF HEPATIC ISCHEMLA-REPERFUSION INJURY AND HEPATIC MICROCIRCULATION

点击翻译按钮获取中文摘要

A protective effects of the SOD (superoxide dismutase)-DIVEMA(divinyl ether and maleic anhydride) conjugate on hepatic ischemia-reperfusion injury was demonstrated. Twenty min. of normothermotic ischemia was induced by clamping the portal triad of Sprangue-Dawley rats. SOD, SOD-DIVEMA, or physiologic NaCl was administered 5 min. prior to reperfusion. Hepatic microcirculation was obserbed during the 120 min. reperfusion period using intravital-fluoresent microscope and events were'recorded to video system for the later analysis. SOD- DIVEMA restored the sinusoidal perfusion rate (98.5% for SOD-DIVEMA vs. 98.0% for no-ischemia, 65.5% for NaCl and 81.5% for SOD administration), and reduced the number of leukocyte stagnant in the acini (12.3 cells/lobule for SOD- DIVEMA vs. 4.4 for no-ischemia, 36.6 for NaCi, 27.7 for SOD administration) and adherent it in the postsinusoidal venule (40.0 cells/mm^2 for SOD-DWEMA vs. 55.0 for no-ischemia, 417 for NaCl and 253 for SOD administration) significantly in comparing control studies. Furthermore, SOD-DIVEMA maintained postischemic hepatocellular integrity . The SOD-DIVEMA treated group maintained significantly higher serum level of SOD activity after 120 min. of reperfusion than the SOD treated group ; 33.0 U/ml vs. 8.6 U/mi. The demonstration of the most beneficial efficacy of SOD-DIVEMA after the period of 120 min. of repercussion indicats a longer intravascular SOD activity than sole SOD administration.