Mucin glycoproteins in human gallbladder carcinoma and their biological effects on tumor invasion and metastasis capability.

人胆囊癌中的粘蛋白糖蛋白及其对肿瘤侵袭和转移能力的生物学作用。

• 批准号: 13671279
• 负责人: TODOROKI Takeshi
• 金额: $ 2.5万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671279/
• 关键词: gallbladder carcinoma; animal model; mucin glycoprotein; oncogene; signal transduction; gallbladder carcinogenesis; 転移・浸潤; 腫瘍生物学; pT2胆嚢癌; MY.1E.12; MUC1ムチン; GalNAcT3; E-cadherin; 予後; 再発形式; MUC 1ムチン; 糖転移酵素; 接着分子; 悪性度; 肝転移モデル; 類洞内皮; 粗血再

The overall prognosis of gallbladder carcinoma is poor because of a high percentage of advanced cases at the time of operation and thereby a lower curability. pT2 carcinoma is limited in the gallbladder wall, and therefore a high curability as well as a better prognosis can be expected. However, the prognosis has not been necessarily good since distant recurrences are found in some cases of pT2 carcinomas. Alterations in glycosilation of mucins, such as an alteration in the site of O-glycosylation of mucin core proteins or an enhanced sialylation of carbohydrate structures, are known in the process of malignant transformation.Sialylated MUC1 recognized by a mAb MY.1E12 (Jpn J Cancer Res 1996;87:488-496) has been used as a marker for invasive adenocarcinomas. In pT2 gallbladder carcinoma, the high level expression of stromal localization of sialylated MY.1E12-reactive-MUC1 at the invading sites correlates with the aggressiveness of the disease, such as the tendency to form distant metastasis.Thus, alterations in carbohydrate chains of carcinoma-producing mucin glycoproteins potentiate the capability of tumor invasion and/or metastasis.

胆囊癌的整体预后较差，因为手术时晚期病例的比例很高，因此治愈率较低。pT 2癌局限于胆囊壁，因此可以预期高治愈率以及更好的预后。然而，预后并不一定好，因为在一些pT 2癌病例中发现远处复发。在恶性转化过程中，已知粘蛋白的糖基化的改变，例如粘蛋白核心蛋白的O-糖基化位点的改变或碳水化合物结构的唾液酸化增强。由mAb MY.1E12识别的唾液酸化MUC 1（Jpn J Cancer Res 1996;87：488-496）已被用作侵袭性腺癌的标志物。在pT 2胆囊癌中，浸润部位的唾液酸化MY.1E12-reactive-MUC 1的间质定位的高水平表达与疾病的侵袭性相关，例如形成远处转移的倾向。因此，产生癌的粘蛋白糖蛋白的糖链的改变增强了肿瘤侵袭和/或转移的能力。

项目成果

Patterns and predictors of failure after curative resections

根治性切除术后失败的模式和预测因素

• 发表时间: 2003
• 期刊: Annals of Surgical Oncology 10
• 作者: Todoroki T et al.

Expression of cyclooxygenase-2 in the subserosal layer.

浆膜下层中环氧合酶 2 的表达。

• 发表时间: 2002
• 期刊: International Journal of Cancer 98
• 作者: Kawamoto T et al.

Toru Kawamoto: "Expression of Cyclooxygenase-2 in the Subserosal Layer Correlates with Postsurgical Prognosis of PT2 Carcinoma of the Gallbladder"International Journal of Cancer. (in press). (2002)

Toru Kawamoto：“浆膜下层环氧合酶 2 的表达与 PT2 胆囊癌的术后预后相关”《国际癌症杂志》。

Expression of MUC1 mucins in the subserosal layer

浆膜下层MUC1粘蛋白的表达

• 发表时间: 2001
• 期刊: Clinical Cancer Research 7
• 作者: Kawamoto T et al.

Toru Kawamoto: "Expression of MUC 1 Mucins in Subserosal Layer Correlates with Postsurgical Prognosis of PT2 Carcinoma of the Gallbladder"Clinical Cancer Research. Vol.7. 1333-1342 (2001)

Toru Kawamoto：“浆膜下层 MUC 1 粘蛋白的表达与 PT2 胆囊癌的术后预后相关”临床癌症研究。