Protection of hepatic microcirculatory disturbance due to the ischemia-reperfusion injury

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Abstract[Background/Aim] A synthesized non-peptide endothelin receptor; SB209670 (SB) reported to inhibit vasoconstriction; however its effects on the post-ischemic liver microcirculation (PILMC) is still unclear. To clarify the effects of SB on the PILMC was the aim of this study.[Material and Methods] We analyses the effects of SB on not only PILMC, but also release of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) and the hepatocyte morphology after ischemia by intravital fluorescent microscopy. [Results] SB significantly inhibited the sinusoidal vasoconstriction, leukocyte sticking and restored the perfusion rate than NaCl administration in comparison with those of non-ischemic liver, 94.1%; vs. 83.8%, 1.54/acini vs. 5.12/acini and 93.3% vs. 70. 2%, respective, however, it did not the vasoconstriction and leukocyte sticking in the post-sinusoidal venule. SB also did not prevent release of liver enzyme and the increase of the vacuolated hepatocyte number. [Conclusion] SB protects the post-ischemic microcirculatory disturbance in the sinusoid, however it did not prevent ischemic reperfusion liver injury provably due to the conflictive effects in the post-sinusoidal venule.