Protection of hepatic microcirculatory disturbance due to the ischemia-reperfusion injury

对缺血再灌注损伤引起的肝脏微循环障碍的保护作用

• 批准号: 13671278
• 负责人: TODOROKI Takeshi
• 金额: $ 2.3万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671278/
• 关键词: liver microcirculation; ischemia-reperfusion injury; endothelin receptor antagonist; central venous flow; sinusoid perfusion; leukocyte adhesion; liver dysfunction; SB209670; 肝阻血再潅流障害; エンドセリン拮抗薬; 中心静脈血行; 胆汁排泄; 肝酵素

Abstract[Background/Aim] A synthesized non-peptide endothelin receptor; SB209670 (SB) reported to inhibit vasoconstriction; however its effects on the post-ischemic liver microcirculation (PILMC) is still unclear. To clarify the effects of SB on the PILMC was the aim of this study.[Material and Methods] We analyses the effects of SB on not only PILMC, but also release of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) and the hepatocyte morphology after ischemia by intravital fluorescent microscopy. [Results] SB significantly inhibited the sinusoidal vasoconstriction, leukocyte sticking and restored the perfusion rate than NaCl administration in comparison with those of non-ischemic liver, 94.1%; vs. 83.8%, 1.54/acini vs. 5.12/acini and 93.3% vs. 70. 2%, respective, however, it did not the vasoconstriction and leukocyte sticking in the post-sinusoidal venule. SB also did not prevent release of liver enzyme and the increase of the vacuolated hepatocyte number. [Conclusion] SB protects the post-ischemic microcirculatory disturbance in the sinusoid, however it did not prevent ischemic reperfusion liver injury provably due to the conflictive effects in the post-sinusoidal venule.

摘要[背景/目的]人工合成的非肽内皮素受体SB209670(SB)具有抑制血管收缩的作用，但其对缺血后肝微循环(PILMC)的影响尚不清楚。[材料与方法]利用活体荧光显微镜观察Sb对PILMC的影响，以及对缺血后肝细胞谷草转氨酶(AST)、丙氨酸氨基转移酶(ALT)、乳酸脱氢酶(LDH)的释放及肝细胞形态的影响。[结果]SB明显抑制肝窦血管收缩、白细胞黏附，恢复灌注率，与非缺血组比较，分别为94.1%、83.8%、1.54个/腺泡和5.12个/腺泡、93.3%和70%。2%未见血管收缩和白细胞粘连于窦后小静脉。SB也不能阻止肝酶的释放和空泡化肝细胞数量的增加。[结论]Sb对缺血后肝窦微循环障碍有保护作用，但由于肝窦后小静脉的相互矛盾作用而不能预防缺血再灌注肝损伤。

项目成果

Expression of Cyclooxygenase-2 in the Subserosal Layer Correlates with Postsurgical Prognosis of Pathological Tumor Stage Carcinoma of the Gallbladder

浆膜下层环氧合酶-2 的表达与胆囊病理肿瘤期癌的术后预后相关

• 期刊: Int.J.Canser 8(4)
• 作者: T.Todoroki

Synthesis and Properties of Poly [N-(2-hydroxyproprl)ethacrylaide] Conjugates of Superoxide Dismutase

超氧化物歧化酶聚[N-(2-羟丙基)乙基丙烯酰胺]结合物的合成及性能

• 发表时间: 2002
• 期刊: Journal of Bioactive and Compatible Polymers 196(13)
• 作者: T.Todoroki

E-cadherin expression in the primary tumors and metastatic lymph nodes of poorly differentiated types of rectal cancer

• DOI: 10.1007/s005950200004
• 发表时间: 2002-01-01
• 期刊: SURGERY TODAY
• 影响因子: 2.5
• 作者: Kanazawa, N;Oda, T;Fukao, K
• 通讯作者: Fukao, K

T.Todoroki: "Synthesis and Properties Poly [N-(2-hydroxypropyl) methacrylamide] Conjugates of Superoxide Dismutase"Journal of Bioactive and Compatible Polymers. 17. 105-122 (2002)

T.Todoroki：“超氧化物歧化酶聚[N-(2-羟丙基)甲基丙烯酰胺]缀合物的合成和特性”生物活性和相容聚合物杂志。

轟健: "消化器外科 12 消化器画像をめぐる話題"へるす出版. 6 (2002)

Ken Todoroki：“胃肠外科 12 个胃肠道图像主题”健康出版 6 (2002)。