REGULATION OF CEREBROSPINAL FLUID CIRCULATION AND INTRACRANIAL PRESSURE BY WATER CHANNEL

水道对脑脊液循环和颅内压的调节

• 批准号: 08671600
• 负责人: MASAGO Atsuo
• 金额: $ 1.41万
• 依托单位: NAGOYA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671600/
• 关键词: intracranial pressure; cerebrospinal fluid; hydrocephalus; osmolyte transporter; water channel

The present study was designed to investigate molecular regulation of cerebrospinal fluid circulation under the high intracranial pressure. The investigators proposed research projects focused on the expression of water channel, osmolyte transporters and natriuretic peptide. The following results were obtained. (1)EXPRESSION OF WATER CHANNEL GENE : Water channel transports water molecules across the cell membrane into or out of the cells. Water channel is supposed to regulate water content in the brain tissue. Using in situ hybridization histochemistry, water channel (MIWC) mRNA was expressed in the ependymal cells and cerebral white matter. However, MIWC mRNA expression did not change in the high intracranial environment and kaoline-induced hydrocephalus in rats. Water channel includes other homologous superfamilies which might control water content in the pathological conditions. (2)C-TYPE NATRIURETIC PEPTIDE (CNP) : CNP is widely present over the central nervous system and plays a r … More ole in the hormonal regulation of systemic blood circulation. The expression of CNP mRNA was changeless after the development of hydrocephalus. The CNP gene might fluctuate within a short period and could not be evaluated satisfactorily. (3)GLUTAMATE/ASPARTATE TRANSPORTER (GLAST) : GLAST plays an important role in regulation of osmotic pressure and extracellular glutamate concentration. In the acute stage of experimental hydrocephalus, periventricular reactive astrocytes markedly expressed GLAST mRNA.High ICP and ventricular dilatation in the acute hydrocephalus cause periventricular edema and neuronal damage. GLAST gene might be induced in order to regulate local osmotic pressure or remove excess glutamate released from injured axons (Masago et al.1996). (4)SUBARACHNOID HEMORRHAGE (SAH) MODEL AND NEURONAL STRESS : We developed original SAH model in rats by endovascular perforation with nylon thread. This model represents closely aneurysmal SAH.We are investigating osmoregulation using this SAH model. Less

本研究旨在探讨高颅压下脑脊液循环的分子调控。研究人员提出了重点研究水通道、渗透压转运蛋白和利钠肽表达的研究项目。获得了以下结果。（1）水通道基因的表达：水通道将水分子穿过细胞膜运输到细胞内或细胞外。水通道被认为调节脑组织中的水含量。用原位杂交组织化学方法检测水通道（MIWC）mRNA在室管膜细胞和大脑白色物质中的表达。然而，MIWC mRNA的表达没有改变，在高颅内环境和高岭土诱导的脑积水大鼠。水通道包括其他同源超家族，它们可能在病理条件下控制水含量。（2）C型利尿钠肽（CNP）：CNP广泛存在于中枢神经系统，在中枢神经系统中起重要作用， ...更多信息 在全身血液循环的激素调节中起作用。CNP mRNA的表达在脑积水发生后无明显变化。CNP基因可能在短时间内波动，无法进行令人满意的评价。（3）谷氨酸/天冬氨酸转运蛋白（GLAST）：GLAST在渗透压和细胞外谷氨酸浓度的调节中起重要作用。实验性脑积水急性期，脑室周围反应性星形胶质细胞表达GLAST mRNA，高ICP和脑室扩张导致脑室周围水肿和神经元损伤。GLAST基因可能被诱导以调节局部渗透压或去除从受损轴突释放的过量谷氨酸（Masago et al.1996）。（4）蛛网膜下腔出血（SUBARACHNOID HEMORRHAGE，SAH）模型及神经元应激：采用尼龙线血管内穿孔法建立大鼠SAH模型。这个模型代表了非常接近的系统性SAH。我们正在研究使用这个SAH模型的神经调节。少

项目成果

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Yamada K、Masago A 等人：“脑缺血 II 中的成熟现象。Ito U、Kirino T、Kuroiwa T、Klatzo I（编辑）”，Springer-Verlag，柏林、海德堡，6 (1997)

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Iwata A., Masago A et al:"Exxpression of basic fibroblast growth factor mRNA after transient focal ischemia:comparison with expression of c-fos,c-jun and hsp70mRNA." J Neurotrauma. 14. 201-210 (1997)

Iwata A.、Masago A 等人：“短暂性局部缺血后碱性成纤维细胞生长因子 mRNA 的表达：与 c-fos、c-jun 和 hsp70 mRNA 表达的比较。”

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