Etiological study of the ossification of the spinal ligament.-The immunological and endocrinological studies about general ossification-

脊柱韧带骨化的病因学研究-一般骨化的免疫学和内分泌学研究-

• 批准号: 08671697
• 负责人: OKAJIMA Yukikazu
• 金额: $ 1.47万
• 依托单位: Department of Orthopaedic Surgery, Toho University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671697/
• 关键词: OPLL; Estrogen; Cultured cell; Cell growth factor; Cytokine; Mononucleocyte; T cell function; CD4 positive T cell

We investigated the effect of estrogen as a potential causative for ossification of the posterior longitudinalligament (OPLL). The serum estrogen level was significantly higher in the OPLL patients than controls. Cultured cells derived from OPLL patients had receptors with a higher affinity for estradiol than cells from controls. Cultured cells derived from OPLL patients responded to stimulation by estradiol, elevated the tritiated thymidine uptake and increased the BGP products.In the study of cell growth factors (TGF beta -1, b-FGF), cultured cells derived from OPLL patients stimulated by estradiol, it was found that productive ratio of TGF beta-1 and b-FGF was increased.We also studied that proliferative response of peripheral mononuclear cell (PMNC). When PMNC was stimulated by mitogens such as phytohemagglutinin (PHA), pokeweed mitogen (PWM), Staphylococcus aureus Cowan I (SACI), and monoclonal antibody such as antiCD3 mAb, the prolifetative response of PMNC from OPLL patients was lower than that from healthy controls. IL-2 production of T cells stimulated by PHA or antiCD3 mAb was lower in OPLL patients than healthy controls.And then PMNC stimulated by PHA or anti CD3 mAb with or without TGF beta-1 or b-FGF.Both TGF beta-1 and b-FGF, in addition of higher concentration, it found that the proliferative response was suppressed.It was studied that proliferative response of CD4 T cells purified from PMNC to anti-CD3 mAb is lower in OPLL patients than in healthy controls, too. And IL-2 production of CD4 T cells was also lower in OPLL patients than in healthy controls.In the Study of cell surface markers, CD3, CD4, CD8, CD19, CD25 were detected by flow cytometry. It was not found between OPLL group and healthy controls that difference about subpopulation of lymphocyte.

我们研究了雌激素作为后纵韧带骨化(OPLL)的潜在致病因素的作用。OPLL患者血清雌激素水平明显高于对照组。来自OPLL患者的培养细胞比来自对照的细胞具有更高的雌二醇亲和力。体外培养的OPLL患者细胞对雌二醇刺激的反应，增加了氚胸苷的摄取，增加了BGP的产生。在对细胞生长因子(转化生长因子β-1、b-成纤维细胞生长因子)的研究中，发现转化生长因子β-1和b-成纤维细胞生长因子的比率增加。我们还研究了外周血单个核细胞(PMNC)的增殖反应。在植物血凝素(PHA)、商陆丝裂原(PWM)、金黄色葡萄球菌Cowan I(SACI)等有丝分裂原和抗CD3单抗等单抗刺激下，OPLL患者PMNC的增殖反应明显低于正常对照组。PHA或抗CD3单抗刺激的OPLL患者T细胞产生IL-2的量明显低于健康对照组，而PHA或抗CD3单抗加或不加转化生长因子β-1或b-FGF刺激PMNC后，发现两者的增殖反应均受到抑制。在细胞表面标志物的研究中，采用流式细胞仪检测CD3、CD4、CD8、CD19、CD25。OPLL组与健康对照组之间淋巴细胞亚群差异无统计学意义。

项目成果

安田ゆりか: "後縦靭帯骨化症における末梢単核球増殖能について" 東日本整形災害外科学会雑誌. 10・4. 436-439 (1998)

Yurika Yasuda：“后纵韧带骨化中的外周单核细胞增殖能力”东日本骨科杂志 10/4（1998）。

Terumasa Manone: "An immunological study on ossification of posterior longetudinal ligament" Investigation Committee Report on the ossification of the spinal ligaments of the Japanese Ministry of Public Health and Welfare. 106-108 (1996)

Terumasa Manone：《后纵韧带骨化的免疫学研究》日本厚生省脊柱韧带骨化调查委员会报告。

Akihito Wada: "An effect of sex steroid hormones in patients with spinal ligament ossification"Orthopaedic Surgery and Traumatlogy. 40. 823-830 (1997)

Akihito Wada：“性类固醇激素对脊柱韧带骨化患者的影响”骨科外科和创伤学。

Akihito Wada: "Affinity of estrogen binding in the cultured spinal ligament cells" Journal of Japan Orthopaedic Association. 69. 440-449 (1995)

和田明仁：“培养的脊柱韧带细胞中雌激素结合的亲和力”日本骨科协会杂志。

Terumasa Manome: "The role of estrogen in ossification of the posterior longitudinal ligament" The Journal of the Japan Spine Research Sosiety. 7(2). 348-355 (1996)

Terumasa Manom​​e：“雌激素在后纵韧带骨化中的作用”日本脊柱研究会杂志。