Etiological study of the ossification of the spinal ligament.-The immunological and endocrinological studies about general ossification-

我们研究了雌激素作为后纵韧带骨化(OPLL)的潜在致病因素的作用。OPLL患者血清雌激素水平明显高于对照组。来自OPLL患者的培养细胞比来自对照的细胞具有更高的雌二醇亲和力。体外培养的OPLL患者细胞对雌二醇刺激的反应，增加了氚胸苷的摄取，增加了BGP的产生。在对细胞生长因子(转化生长因子β-1、b-成纤维细胞生长因子)的研究中，发现转化生长因子β-1和b-成纤维细胞生长因子的比率增加。我们还研究了外周血单个核细胞(PMNC)的增殖反应。在植物血凝素(PHA)、商陆丝裂原(PWM)、金黄色葡萄球菌Cowan I(SACI)等有丝分裂原和抗CD3单抗等单抗刺激下，OPLL患者PMNC的增殖反应明显低于正常对照组。PHA或抗CD3单抗刺激的OPLL患者T细胞产生IL-2的量明显低于健康对照组，而PHA或抗CD3单抗加或不加转化生长因子β-1或b-FGF刺激PMNC后，发现两者的增殖反应均受到抑制。在细胞表面标志物的研究中，采用流式细胞仪检测CD3、CD4、CD8、CD19、CD25。OPLL组与健康对照组之间淋巴细胞亚群差异无统计学意义。

We investigated the effect of estrogen as a potential causative for ossification of the posterior longitudinalligament (OPLL). The serum estrogen level was significantly higher in the OPLL patients than controls. Cultured cells derived from OPLL patients had receptors with a higher affinity for estradiol than cells from controls. Cultured cells derived from OPLL patients responded to stimulation by estradiol, elevated the tritiated thymidine uptake and increased the BGP products.In the study of cell growth factors (TGF beta -1, b-FGF), cultured cells derived from OPLL patients stimulated by estradiol, it was found that productive ratio of TGF beta-1 and b-FGF was increased.We also studied that proliferative response of peripheral mononuclear cell (PMNC). When PMNC was stimulated by mitogens such as phytohemagglutinin (PHA), pokeweed mitogen (PWM), Staphylococcus aureus Cowan I (SACI), and monoclonal antibody such as antiCD3 mAb, the prolifetative response of PMNC from OPLL patients was lower than that from healthy controls. IL-2 production of T cells stimulated by PHA or antiCD3 mAb was lower in OPLL patients than healthy controls.And then PMNC stimulated by PHA or anti CD3 mAb with or without TGF beta-1 or b-FGF.Both TGF beta-1 and b-FGF, in addition of higher concentration, it found that the proliferative response was suppressed.It was studied that proliferative response of CD4 T cells purified from PMNC to anti-CD3 mAb is lower in OPLL patients than in healthy controls, too. And IL-2 production of CD4 T cells was also lower in OPLL patients than in healthy controls.In the Study of cell surface markers, CD3, CD4, CD8, CD19, CD25 were detected by flow cytometry. It was not found between OPLL group and healthy controls that difference about subpopulation of lymphocyte.