Etiological study of the ossification of the spinal ligament.-The immunological and endocrinological studies about general ossification-

我们研究了雌激素作为后韧带骨化（OPLL）潜在病因的作用。OPLL患者血清雌激素水平明显高于对照组。来自OPLL患者的培养细胞具有比来自对照的细胞对雌二醇具有更高亲和力的受体。OPLL患者体外培养细胞对雌二醇刺激有反应，能增加对氚标记胸苷的摄取，增加BGP产物的生成，在细胞生长因子（TGF β-1，b-FGF）的研究中，发现OPLL患者体外培养细胞对雌二醇刺激后，TGF β-1和b-FGF的生成率增加，同时还研究了外周血单个核细胞（PMNC）的增殖反应。用植物血凝素（PHA）、美洲商陆（PWM）、金黄色葡萄球菌科万I（SACI）和抗CD 3单克隆抗体等促分裂剂刺激OPLL患者的PMNC，其增殖反应低于健康对照。OPLL患者T细胞经PHA或抗CD 3单抗刺激后IL-2的产生低于正常对照组，而PMNC经PHA或抗CD 3单抗刺激后，TGF β 1或b-FGF的产生均低于正常对照组，TGF β 1和b-FGF的产生均高于正常对照组，且两者的浓度均高于正常对照组。研究了从PMNC中纯化的CD 4 T细胞对抗CD 4 T细胞抗体的增殖反应。OPLL患者的CD 3 mAb也低于健康对照组。流式细胞术检测OPLL患者外周血CD 4 T细胞表面标志物CD 3、CD 4、CD 8、CD 19、CD 25。OPLL组淋巴细胞亚群与健康对照组无明显差异。

We investigated the effect of estrogen as a potential causative for ossification of the posterior longitudinalligament (OPLL). The serum estrogen level was significantly higher in the OPLL patients than controls. Cultured cells derived from OPLL patients had receptors with a higher affinity for estradiol than cells from controls. Cultured cells derived from OPLL patients responded to stimulation by estradiol, elevated the tritiated thymidine uptake and increased the BGP products.In the study of cell growth factors (TGF beta -1, b-FGF), cultured cells derived from OPLL patients stimulated by estradiol, it was found that productive ratio of TGF beta-1 and b-FGF was increased.We also studied that proliferative response of peripheral mononuclear cell (PMNC). When PMNC was stimulated by mitogens such as phytohemagglutinin (PHA), pokeweed mitogen (PWM), Staphylococcus aureus Cowan I (SACI), and monoclonal antibody such as antiCD3 mAb, the prolifetative response of PMNC from OPLL patients was lower than that from healthy controls. IL-2 production of T cells stimulated by PHA or antiCD3 mAb was lower in OPLL patients than healthy controls.And then PMNC stimulated by PHA or anti CD3 mAb with or without TGF beta-1 or b-FGF.Both TGF beta-1 and b-FGF, in addition of higher concentration, it found that the proliferative response was suppressed.It was studied that proliferative response of CD4 T cells purified from PMNC to anti-CD3 mAb is lower in OPLL patients than in healthy controls, too. And IL-2 production of CD4 T cells was also lower in OPLL patients than in healthy controls.In the Study of cell surface markers, CD3, CD4, CD8, CD19, CD25 were detected by flow cytometry. It was not found between OPLL group and healthy controls that difference about subpopulation of lymphocyte.