Common hormones and mechanisms of luteal regression, ovulation and follicular atresia.

我们研究了黄体退化、排卵和卵泡闭锁的常见激素及其机制。我们之前报道过，在溴隐碱（Brom）治疗后，催乳素（PRL）治疗超排卵大鼠引起结构性黄体溶解，并且这种治疗刺激了累及黄体的基质金属蛋白酶活性并引起细胞凋亡。我们旨在探讨除PRL外的其他激素是否会引起大鼠结构性黄体溶解，并探讨人类黄体退化的机制。我们得到了一些优秀的数据如下。Brom处理后的生长激素处理通过MMP激活和细胞凋亡引起结构性黄体溶解。注射hCG后第5天至第9天使用促性腺激素释放激素激动剂（GnRHa）也引起大鼠结构性黄体溶解。明胶酶谱分析、Northem blot分析显示，GnRHa处理刺激了MMP-2、MT-MMP活性。黄体晚期提取物的MMP-2、MT1-MMP活性明显高于其他期提取物。黄体组织金属蛋白酶抑制剂(TIMP)-1的表达在黄体晚期降低。而TIMP-2的表达在黄体晚期略有升高。可能如先前在其他细胞中报道的那样，在TIMP-2存在的情况下，MT1-MMP激活了前mmp -2。很明显，黄体退化、排卵和卵泡闭锁具有上述相同的机制。

We investigated common hormones and mechanisms of luteal regression, ovulation and follicular atresia. We previously reported that prolactin (PRL) treatment following bromocryptine (Brom) treatment to superovulated rats caused structural luteolysis, and that this treatment stimulated matrix metalloproteinase activity in involuted corpus luteum and caused apoptosis.We designed to investigate whether other hormones except PRL may cause structural luteolysis in rats, and also investigated about mechanisms of luteal regression in human. We got some exellent data as below.Growth hormone treatment following Brom treatment caused structural luteolysis by MMP activation and apoptosis. Gonadotropin releasing hormone agonist (GnRHa) treatment from day 5 through day 9 after hCG injection also caused structural luteolysis in rats. Gelatin zymography, Northem blot analysis revealed that GnRHa treatment stimulated MMP-2, MT-MMP activity.Extracts of human corpus luteum of late luteal phase had much more activities of MMP-2, MT1-MMP than those of other stages. Tissue inhibitor of metalloproteinse (TIMP)-1 expression of corpus luteum decreased at late luteal phase. However, TIMP-2 expression slightly increased at late luteal phase compared with other stages. It is possible that MT1-MMP activates pro-MMP-2 under the existence of TIMP-2 as previously reported in other kinds of cells.It became clear that luteal regression, ovulation and follicular atresia have same kinds of mechanisms as mentioned above.