A role of keratinocyte growth factor on wound healing of oral epithelium

角质细胞生长因子对口腔上皮伤口愈合的作用

• 批准号: 08672316
• 负责人: IGA Hiroki
• 金额: $ 1.6万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672316/
• 关键词: KGF; Oral Epithelium; Keratinocyte; Odontgenic Keratocyst; 歯原性角化嚢胞

Keratinocyte growth factor (KGF) is a paracrine growth factor produced by fibroblasts and specifically mitogenic for epithelial cells. Recently, it was reported that KGF may have an important role in stimulating re-epithlialization during the process of wound healing of the skin. However, the role of KGF on human oral mucosa has not been investigated.In this study, we investigated the effect of KGF on cell proliferation and migration of normal human keratinocytes derived from oral mucosa. We demonstrated the stimulatory effect of KGF on keratinocyte proliferation by MTT assay. After removing the half of confluent monolayrs of keratinocytes using razer blade, we observed original edge of the wound by phase-contrast microscopy (Migration assay). Incubation of human oral keratinocytes with KGF significantly stimulated the cell migration. These results indicate that KGF may play an important role on wound healing in human oral mucosa as well as skin. However, we could not detect KGF protein in fibroblasts from oral mucosa using immunofluorescene staining. Moreover, KGF mRNA was hardly detected in normal human oral mucosa by in situ hybridization.On the other hand, we investigated the expression of KGF in several cystic lesions by immunohistochemical staining technique. Consequently, KGF was detected in the cyst wall of odontogenic keratocysts, but not in dentigerous cyst. This result also indicates that KGF may be one of the important factors which cause a certain oral disease.

角质形成细胞生长因子 (KGF) 是一种由成纤维细胞产生的旁分泌生长因子，对上皮细胞具有特异性促有丝分裂作用。最近有报道称，KGF可能在皮肤伤口愈合过程中刺激上皮细胞再生方面发挥重要作用。然而，KGF对人口腔粘膜的作用尚未被研究。在本研究中，我们研究了KGF对源自口腔粘膜的正常人角质形成细胞的细胞增殖和迁移的影响。我们通过 MTT 测定证明了 KGF 对角质形成细胞增殖的刺激作用。使用剃刀刀片去除一半融合的单层角质形成细胞后，我们通过相差显微镜（迁移测定）观察伤口的原始边缘。将人口腔角质形成细胞与 KGF 一起孵育可显着刺激细胞迁移。这些结果表明KGF可能对人类口腔粘膜和皮肤的伤口愈合发挥重要作用。然而，我们无法使用免疫荧光染色检测口腔粘膜成纤维细胞中的 KGF 蛋白。而且，通过原位杂交，在正常人口腔粘膜中几乎检测不到KGF mRNA。另一方面，我们通过免疫组化染色技术研究了KGF在几种囊性病变中的表达。因此，在牙源性角化囊肿的囊壁中检测到 KGF，但在含牙囊肿中未检测到。这一结果也表明KGF可能是引起某种口腔疾病的重要因素之一。

项目成果

Sato, M: "Emergence of osteblast-like cells in a neoplastic human salivary cancer cell line after treatment with 22-oxa-1d,25-dihydroxyuitamirD_3" Cancer Letter. 115. 149-160 (1997)

Sato, M：“用 22-oxa-1d,25-diHydroxyuitamirD_3 治疗后，肿瘤性人唾液癌细胞系中出现成骨细胞样细胞”《癌症通讯》。

Sato M,et al.: "Emergene of osteoblast-like cells in a neoplastic human salivary cancer cell line after treatment with 22-oxa-1alpha, 25-dihydroxyvitaminD_3." Cancer Letter. 115. 149-160 (1997)

Sato M 等人：“用 22-oxa-1α、25-二羟基维生素 D_3 处理后，肿瘤性人唾液癌细胞系中成骨细胞样细胞的出现。”

Sato,M.: "Emargence of osteoblast-like cells in a neoplastic human salivary cancer cell line after treatment with 22-oxa-1d,25-dihydroxyuitamin D_3" Cancer Letter. 115. 149-160 (1997)

Sato,M.：“用 22-oxa-1d,25-二羟基维生素 D_3 治疗后，肿瘤性人唾液癌细胞系中成骨细胞样细胞的出现”《癌症通讯》。