Analysis on expression of tumor related antigen and its clinical application in oral cancers.

应用着丝粒周围特异性DNA探针荧光原位杂交（FISH）技术，对27例口腔鳞癌组织中17号染色体数目畸变进行了研究，并探讨了其与p53和Bcl-2蛋白表达的关系。17号染色体异常与口腔鳞癌的发生有关（p=0.0005），其异常程度与口腔鳞癌的分期、分化程度、复发及淋巴结转移无关。然而，多体性17和p53免疫反应性之间存在相关性（p=0.0228）。p53免疫反应阳性率与肿瘤复发相关（p= 0.0441）。p53与Bcl-2免疫反应呈负相关（p= 0.0421）。总之，我们认为，17号染色体多态性与口腔鳞癌的发生密切相关，17号染色体多态性与p53过表达显著相关。这种现象的可能机制是：17号染色体多态性与p53突变有关，p53突变导致异常p53蛋白的积累，其激活引起p53蛋白的增加。

Numerical aberrations of chromosome 17 was studied by fluorescence in situ hybridization (FISH) using pericentromere specific DNA probe in 27 oral squamous cell carcinoma (SCC), and its relationship with p53 and Bcl-2 protein expression was investigated. Since cells with polysomy 17 significantly increased in SCC (p=0.0005), chromosome 17 abnormality seems to be correlated with carcinogenesis of oral SCC.Chromosome 17 abnormality varied from case to case, and the degree of the abnormality did not correlate with the stage of the disease, the histological differentiation of SCC, relapse of the disease and lymph node matastasis . However, there was the correlation between polysomy 17 and p53 immunoreactivity (p=0.0228). p53 immunoreactivity showed correlation with relapse of the disease (p=0.044 1). An inverse relationship was found between p53 and Bcl-2 immunoreactivity (p=0.042 1). In conclusion, we suggest that polysomy 17 is in close relation to carcinogenesis of oral SCC, and that polysomy 17 significantly correlated with p53 overexpression. The possible mechanism of this phenomenon is assumed that polysomy 17 correlates with mutation of p53 which results in an accumulation of aberrant p53 protein, and that its activation causes an increase of p53 protein.