Analysis on expression of tumor related antigen and its clinical application in oral cancers.

口腔癌肿瘤相关抗原表达分析及其临床应用

• 批准号: 08672312
• 负责人: TSUJI Tatsuo
• 金额: $ 1.34万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672312/
• 关键词: FISH; p53; Bcl-2; chromosome17; CGH; squamous cell carcinoma; chromosome 3; chromosome 17

Numerical aberrations of chromosome 17 was studied by fluorescence in situ hybridization (FISH) using pericentromere specific DNA probe in 27 oral squamous cell carcinoma (SCC), and its relationship with p53 and Bcl-2 protein expression was investigated. Since cells with polysomy 17 significantly increased in SCC (p=0.0005), chromosome 17 abnormality seems to be correlated with carcinogenesis of oral SCC.Chromosome 17 abnormality varied from case to case, and the degree of the abnormality did not correlate with the stage of the disease, the histological differentiation of SCC, relapse of the disease and lymph node matastasis . However, there was the correlation between polysomy 17 and p53 immunoreactivity (p=0.0228). p53 immunoreactivity showed correlation with relapse of the disease (p=0.044 1). An inverse relationship was found between p53 and Bcl-2 immunoreactivity (p=0.042 1). In conclusion, we suggest that polysomy 17 is in close relation to carcinogenesis of oral SCC, and that polysomy 17 significantly correlated with p53 overexpression. The possible mechanism of this phenomenon is assumed that polysomy 17 correlates with mutation of p53 which results in an accumulation of aberrant p53 protein, and that its activation causes an increase of p53 protein.

应用荧光原位杂交(FISH)技术检测27例口腔鳞状细胞癌(SCC)中17号染色体数目异常，并探讨其与P53、Bcl2蛋白表达的关系。17号染色体异常与口腔鳞癌的发生密切相关(p=0.0005)，17号染色体异常的程度与口腔鳞癌的分期、分化程度、复发及淋巴结转移无关。但P53免疫反应与17号染色体多态之间存在相关性(p=0.0228)。P53阳性表达与肿瘤复发有关(p=0.044 1)。P53与Bcl2表达呈负相关(p=0.042 1)。综上所述，我们认为17号多倍体与口腔鳞状细胞癌的发生密切相关，并且17号多倍体与P53的过度表达显著相关。这种现象的可能机制是假设多倍体17与P53突变有关，突变导致异常的P53蛋白积聚，其激活导致P53蛋白的增加。

项目成果

辻 龍雄: "口腔癌における染色体異常およびp53遺伝子異常." Cytometry Research. 8・1. 1-5 (1998)

Tatsuo Tsuji：“口腔癌中的染色体异常和 p53 基因异常。”8・1（1998）。

Akihiro Kanekawa, Sasaki, Fumihiko Shinozaki.: "Chromosome 17 abnormality in squamous cell carcinoma of the oral cavity, and its relatiohship with p53 and Bcl-2 expression." Anticancer Research. 19 (1) (accepted).(1999)

Akihiro Kanekawa、Sasaki、Fumihiko Shinozaki.：“口腔鳞状细胞癌中的 17 号染色体异常及其与 p53 和 Bcl-2 表达的关系。”

Tatsuo Tsuji: "Increased polysomy 3 and 17 detected by fluorescence in situ hybridization (FISH) in oral squamous cell carcinoma." Oncology Reports. 4. 277-280 (1997)

Tatsuo Tsuji：“通过荧光原位杂交 (FISH) 检测口腔鳞状细胞癌中多体性 3 和 17 的增加。”

辻 龍雄: "口腔癌における染色体異常・p53遺伝子異常" Cytometry Research. 8. (1998)

Tatsuo Tsuji：“口腔癌中的染色体异常和 p53 基因异常”细胞计数研究 8。（1998）

Akihiro Kanekawa: "Chromosome 17 abnormality in squamous cell carcinoma of the oral cavity,and its relatiohship with p53 and Bcl-2 expression." Anticancer Research. 19・1(掲載予定). (1999)

Akihiro Kanekawa：“口腔鳞状细胞癌的 17 号染色体异常及其与 p53 和 Bcl-2 表达的关系。抗癌研究”（待出版）。