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ANALYSIS ON EXPRESSION OF TUMOR RELATED ANTIGEN AND ITS CLINICAL APLLICATION IN ORAL CANCERS.

口腔癌肿瘤相关抗原的表达及其临床应用分析。

基本信息

批准号：
05671671
负责人：
TSUJI Tatsuo
金额：
$ 1.34万
依托单位：
YAMAGUCHI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671671/
关键词：
IMMUNOHISTOCHEMISTRY FISH p53 PCNA DNA PLOIDY ORAL CANCER Oral camcer Immunohisto chemistry chromosome 17 p53癌抑制遺伝子増殖細胞核抗原 DNA aneuploidy フローサイトメトリー免疫組織化学口腔癌

项目摘要

(1) The immunohistochemical staining of p53 protein was observed in cancer tissues and normal epithelium. Immunoriactivity of p53 and PCNA showed similarities in staining on sqaumous cell carcinoma of the oral cavity, and that p53 score was smaller than PCNA score.(2) The expression of p53 protein was commonly involved in malignant salivary gland tumor and the p53 positive rate was different among various histological types of malignant salivary gland tumors.(3) Numerical aberrations of chromosomes were investigate in oral tumors by fluorescence in situ hybridization, and suggested that numerical aberrations of chromosme occur during the development of malignant tumors of the oral cavity.(4) The expression of tenascin was observed not only tumor cells but also subepithelial border and in various salivary gland tumors. Therefore, it seemed to be implicated in these phenomena.(5) The expression of p53 protein and PCNA was immunohistochemically examined. The immunoreactivity for p53 prote … More in wwas defferent among various malignant salivary gland tumors. p53 protein was more frequently detectable in PCNA positive cases than in negative cases.(6) The cytoplasmic p53 protein expression was observed in the normal cells adjacent to p53 positive carcinomas, but none of the normal cells were positive in the tissue surrounding p53 negative carcinomas. Cytoplasmic expression of p53 protein in salivary gland tissues seems to be correlated with tumorigenesis.(7) The PCNA labeling index was higher in squamous cell carcinomas than in other benign oral lesions. Survival curve of the patients who were negative for p53 was significantly more favorable than for patients who were positive for p53.(8) Polysomy of both chromosomes occurs during the development of salivary gland tumors, and its frequency is increased in adenoid cystic carcinoma as compared to pleomorphic adenoma. A single hybridised signal was much more frequent for chromosome 17 than for chromosome 3. Monosomy of chromosome 17 could possibly be significant in salivary gland tumors.(9) Both of PCNA labeling index and AgNORs counts were significantly higher in squamous cell carcinomas than in leukoplakias. PCNA labeling index apparently increased in leukoplakias with dysplasia and malignant transformed cases.(10) Polysomy of chromosome 3 and 17 occurs during the development of oral tumors. However, the pattern of aneusomy was different among squamous cell carcinomas and salivary gland tumors. Monosomy of chromosome 17 seems to be speciffic in salivary gland tumors. Less

(1)p53蛋白在癌组织和正常上皮中的免疫组化染色。p53和PCNA在口腔鳞癌中的表达相似，p53评分小于PCNA评分。(2)p53蛋白的表达在涎腺恶性肿瘤中普遍存在，且在不同组织学类型的涎腺恶性肿瘤中阳性率不同。(3)应用荧光原位杂交技术研究了口腔恶性肿瘤中染色体数目的畸变，提示口腔恶性肿瘤的发生发展过程中存在染色体数目畸变。(4)腱生蛋白不仅在涎腺肿瘤细胞中表达，而且在上皮下缘及各种涎腺肿瘤中均有表达。所以，这一切似乎都与它有关。(5)免疫组化法检测p53蛋白和PCNA的表达。p53蛋白的免疫反应性 ...更多信息不同类型涎腺恶性肿瘤的组织学分级不同。PCNA阳性组p53蛋白表达明显高于阴性组。(6)p53阳性癌旁正常细胞胞浆内有p53蛋白表达，而p53阴性癌旁正常细胞胞浆内无p53蛋白表达。涎腺组织中p53蛋白的胞浆表达与肿瘤的发生有关。(7)PCNA标记指数在鳞状细胞癌中高于其他口腔良性病变。p53阴性患者的生存曲线明显优于p53阳性患者。(8)在涎腺肿瘤的发展过程中，两条染色体的多体性发生，与多形性腺瘤相比，其在腺样囊性癌中的频率增加。一个单一的杂交信号是更频繁的染色体17比染色体3。17号染色体单体性在涎腺肿瘤中可能有意义。(9)鳞状细胞癌PCNA标记指数和AgNORs计数均显著高于白斑。增殖细胞核抗原（PCNA）标记指数在白斑伴异型增生和恶变病例中明显增高。(10)在口腔肿瘤的发展过程中，3号和17号染色体发生了多体性。然而，异染色体的模式是不同的鳞状细胞癌和涎腺肿瘤。17号染色体单体性在涎腺肿瘤中可能是特异性的。少