ANALYSIS ON EXPRESSION OF TUMOR RELATED ANTIGEN AND ITS CLINICAL APLLICATION IN ORAL CANCERS.

(1)癌组织和正常上皮中p53蛋白免疫组化染色。在口腔鳞癌的染色中，p53与PCNA的免疫活性相似，且p53评分低于PCNA评分。(2)恶性唾液腺肿瘤中p53蛋白的表达普遍参与，不同组织学类型的恶性唾液腺肿瘤中p53阳性率存在差异。(3)应用荧光原位杂交技术对口腔肿瘤中染色体数值畸变进行了研究，发现在口腔恶性肿瘤的发展过程中存在染色体数值畸变。(4) tenascin不仅在肿瘤细胞中表达，而且在上皮下边界和各种唾液腺肿瘤中均有表达。因此，它似乎与这些现象有关。(5)免疫组化检测p53蛋白和PCNA的表达。不同涎腺恶性肿瘤对p53蛋白的免疫反应性差异较大。p53蛋白在PCNA阳性病例中的检测频率高于阴性病例。(6) p53阳性癌旁正常细胞胞质中有p53蛋白表达，而p53阴性癌旁正常细胞胞质中无p53蛋白表达。唾液腺组织中p53蛋白的细胞质表达似乎与肿瘤发生有关。(7)鳞状细胞癌的PCNA标记指数高于其他口腔良性病变。p53阴性患者的生存曲线明显优于p53阳性患者。(8)在唾液腺肿瘤的发展过程中出现双染色体多体，在腺样囊性癌中出现的频率高于多形性腺瘤。单个杂交信号在第17号染色体上比在第3号染色体上更频繁。第17号染色体单体可能在唾液腺肿瘤中具有重要意义。(9)鳞状细胞癌的PCNA标记指数和AgNORs计数均显著高于白斑。PCNA标记指数在白斑伴不典型增生及恶变病例中明显升高。（10）口腔肿瘤发生过程中出现3、17号染色体多体。然而，鳞状细胞癌和唾液腺肿瘤的解剖模式不同。第17号染色体的单体似乎在唾液腺肿瘤中具有特异性。少

(1) The immunohistochemical staining of p53 protein was observed in cancer tissues and normal epithelium. Immunoriactivity of p53 and PCNA showed similarities in staining on sqaumous cell carcinoma of the oral cavity, and that p53 score was smaller than PCNA score.(2) The expression of p53 protein was commonly involved in malignant salivary gland tumor and the p53 positive rate was different among various histological types of malignant salivary gland tumors.(3) Numerical aberrations of chromosomes were investigate in oral tumors by fluorescence in situ hybridization, and suggested that numerical aberrations of chromosme occur during the development of malignant tumors of the oral cavity.(4) The expression of tenascin was observed not only tumor cells but also subepithelial border and in various salivary gland tumors. Therefore, it seemed to be implicated in these phenomena.(5) The expression of p53 protein and PCNA was immunohistochemically examined. The immunoreactivity for p53 prote … More in wwas defferent among various malignant salivary gland tumors. p53 protein was more frequently detectable in PCNA positive cases than in negative cases.(6) The cytoplasmic p53 protein expression was observed in the normal cells adjacent to p53 positive carcinomas, but none of the normal cells were positive in the tissue surrounding p53 negative carcinomas. Cytoplasmic expression of p53 protein in salivary gland tissues seems to be correlated with tumorigenesis.(7) The PCNA labeling index was higher in squamous cell carcinomas than in other benign oral lesions. Survival curve of the patients who were negative for p53 was significantly more favorable than for patients who were positive for p53.(8) Polysomy of both chromosomes occurs during the development of salivary gland tumors, and its frequency is increased in adenoid cystic carcinoma as compared to pleomorphic adenoma. A single hybridised signal was much more frequent for chromosome 17 than for chromosome 3. Monosomy of chromosome 17 could possibly be significant in salivary gland tumors.(9) Both of PCNA labeling index and AgNORs counts were significantly higher in squamous cell carcinomas than in leukoplakias. PCNA labeling index apparently increased in leukoplakias with dysplasia and malignant transformed cases.(10) Polysomy of chromosome 3 and 17 occurs during the development of oral tumors. However, the pattern of aneusomy was different among squamous cell carcinomas and salivary gland tumors. Monosomy of chromosome 17 seems to be speciffic in salivary gland tumors. Less