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Analysis of expression of tumor-associated antigen and its clinical application in oral cancers.

口腔癌肿瘤相关抗原的表达分析及其临床应用

基本信息

批准号：
02670898
负责人：
TSUJI Tatsuo
金额：
$ 1.6万
依托单位：
Yamaguchi University Hospital
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

The numerical aberration of chromosome 17 can be detected by fluorescence in situ hybridization in oral tumors and this correlated with malignant behavior of oral tumors. PCNA immunoreactivity correlated with the differentiation and atypia of the tumor cells in squamous cell carcinoma of the oral cavity. PCNA score was significantly higher in the malignant and pre-malignant lesion, and correlate with the degree of dysplasia in pre-malignant lesions. The mean PCNA score decreased significantly after cancer chemotherapy of squamous cell carcinoma. According to this results, the response of cancer cells to anticancer agents may be estimated by consecutive measurement of PCNA. c-erbB-2 oncoprotein is rarely expressed in malignant salivary gland tumors. However, with a limited number of c-erbB-2 oncoprotein positive cases, it was not possible to correlate the oncogene status with clinical behavior of the tumor. c-erbB-2 did not detected in squamous cell carcinoma, but markedly detected in plemorphic adenoma, and especially in the cells which showed invasive growth activity DNA aneuploidy is not specific for malignant tumors and there was significant difference in DNA index between malignant and benign ledsions. KM-93 antigen detected significantly high level in the patient serum of the salivary gland tumotrs, therefore, it seems that measurement of this antigen is useful method for clinical practice.

荧光原位杂交技术可检测到口腔肿瘤组织中17号染色体的数值畸变，这与口腔肿瘤的恶性行为有关。口腔鳞状细胞癌PCNA免疫反应性与肿瘤细胞的分化和异型性相关。PCNA评分在恶性及癌前病变中均显著增高，且与癌前病变发育不良程度相关。鳞状细胞癌化疗后PCNA平均评分明显降低。根据这一结果，可以通过连续测量PCNA来估计癌细胞对抗癌药物的反应。c-erbB-2癌蛋白在恶性唾液腺肿瘤中很少表达。然而，由于c-erbB-2癌蛋白阳性病例数量有限，因此不可能将癌基因状态与肿瘤的临床行为联系起来。c-erbB-2在鳞状细胞癌中未检出，但在多形性腺瘤中检出明显，尤其在具有侵袭性生长活性的细胞中，DNA非整倍体对恶性肿瘤无特异性，且在恶性和良性病变中DNA指数有显著差异。KM-93抗原在唾液腺肿瘤患者血清中检测到明显高水平，因此，该抗原的测定似乎是临床实践中有用的方法。