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Expression and function of Fas antigen in human oral epithelium.

Fas 抗原在人口腔上皮中的表达和功能。

基本信息

批准号：
08672324
负责人：
FUKUDA Jinichi
金额：
$ 1.22万
依托单位：
Kyushu Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672324/
关键词：
Fas antigen Fas ligand apoptosis oral cancer apoptosis Fas antigen gingira immunohistochemistry oral diseases oral cancer immunobloHing oral epithelia

项目摘要

The Fas antigen is a cell-surface glycoprotein that mediates apoptosis from the cell surface into the cytoplasm. A monospecific and high titer polyclonal antibody, designated as Fas D,was raised against synthetic polypeptides selected from a part of the human Fas antigen (amino acid resideues 104-114). This antibody was used to identify the Fas antigen in human oral epithelia in normal and pathological states. Biopsy specimens of normal human gingiva were prepared and the paraffin sections were reacted with the Fas D antibody by an immunohistochemical method. Specimens obtained from patients with odontogenic Keratocysts, leukoplakia, lichen planus, and squamous cell carcinoma were also stained with the antibody. The pattern of the Fas antigen distribution in oral stratified squamous epithelia was, with some overlapping, characteristic for each disease. The antibody localized to the prickle-cell layr and to granular layr of keratinocytes of human gingiva. Proteins were also prepared from human gingiva and subjected to SDS-PAGE,followed by Western blotting analysis with the Fas D antibody. The antibody interacted with a band cprresponding to an estimated molecular weight of 35 kDa. The incidence of the immunoreactive 35-kDa protein was detected in the gingiva of 90% of the 20 individuals examined. The localization of F as antigen in human oral squamous cell carcinoma also was examined. Out of 24 cases of the well-defferentiated tumors examined, 22 showed Fas-positive staining. Among the moderately differentiated tumors, four out of 11 cases showed Fas-positive staining. No Fas-positive cells were observed in the poorly differentiated tumors, but only three specimens were examined. The Fas antigen detected in human oral epithelia may be related to the physiological turnover of oral mucasa and pathological states in oral mucosa. The expression of Fas antigen also appears to be related to the degree of tumor differentiation.

Fas抗原是一种细胞表面糖蛋白，介导细胞凋亡从细胞表面进入细胞质。本文制备了一种单特异性高滴度的多克隆抗体，命名为Fas D，其抗人Fas抗原的一部分（氨基酸残基104-114）的合成多肽。用该抗体检测正常和病理状态下人口腔上皮细胞中Fas抗原的表达。制备正常人牙龈活检标本，石蜡切片用免疫组织化学方法与Fas D抗体反应。从牙源性角化囊肿、白斑、扁平苔藓和鳞状细胞癌患者中获得的标本也用抗体染色。Fas抗原在口腔复层鳞状上皮中的分布模式是每种疾病的特征，但有一些重叠。抗体定位于人牙龈角质形成细胞的棘细胞层和颗粒层。还从人牙龈制备蛋白质，并进行SDS-PAGE，然后用Fas D抗体进行蛋白质印迹分析。该抗体与一条对应于估计分子量为35 kDa的条带相互作用。免疫反应性35-kDa蛋白的发病率检测到90%的20个人检查的牙龈。并对F抗原在人口腔鳞状细胞癌中的定位进行了研究。在24例分化良好的肿瘤中，22例Fas阳性。在中分化肿瘤中，11例中有4例Fas阳性。在低分化肿瘤中未观察到Fas阳性细胞，但仅检查了3例标本。Fas抗原在口腔粘膜上皮细胞中的表达可能与口腔粘膜的生理更新和病理状态有关。Fas抗原表达与肿瘤分化程度有关。