Fas antigen and Fas ligand with Oral Disease

Fas抗原和Fas配体与口腔疾病

• 批准号: 10671900
• 负责人: FUKUDA Jinichi
• 金额: $ 1.98万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671900/
• 关键词: Fas antigen; Fas ligand; apoptosis; oral cancer

To determine the relationship between Fas antigen and apoptosis in oral epithelium, we investigated the expression of Fas antigen in oral mucosa by immunohistochemical and immunoblotting methods.Fas antigen distributed in stratum spinosum and basal part of the stratum coreneum in normal human gingiva. The estimated molecular weight of Fas antigen was calculated as 35,000, which consistent with the value reported for the Fas antigen. Expression of Fas antigen was related to the degree of the tumor differentiation.Using RT-PCR, messenger RNA for Fas antigen was detected in the human oral squamous cell carcinoma cell line, SCC-25. In serum-free medium, a monoclonal ante-Fas antibody induced expression of Fas antigen in SCC-25 cells. Fas antigen was localized to the cytoplasm and the cell membrane. The molecular weight of the protein recognized was 35,000. Anti-Fas monoclonal antibody induced cytotoxicity in SCC-25 cells in a time-dependent manner up to 8 h. Marked nuclear condensation and fragmentation of chromatin were observed in the anti-Fas monoclonal antibody-treated cells using Hoechst 33342 staining. This antibody also induced DNA ladder formation in SCC-25 cells in a time-dependent manner. The present results indicate that the anti Fas monoclonal antibody may mediate apoptosis by binding to the Fas antigen expressed in SCC-cells.

为探讨Fas抗原与口腔上皮细胞凋亡的关系，采用免疫组织化学和免疫印迹方法对Fas抗原在口腔黏膜中的表达进行了研究。Fas抗原的估计分子量为35,000，与报道的Fas抗原的值一致。Fas抗原的表达与肿瘤的分化程度有关，利用RT-PCR技术，检测了口腔鳞癌细胞株SCC-25中Fas抗原的信使RNA。在无血清培养条件下，抗Fas单抗可诱导SCC-25细胞表达Fas抗原。Fas抗原定位于细胞质和细胞膜。识别的蛋白质的相对分子质量为35,000。抗Fas单抗对SCC25细胞的杀伤作用呈时间依赖性，作用时间长达8h，Hoechst 33342染色显示细胞核固缩，染色质断裂。该抗体还以时间依赖的方式诱导SCC-25细胞DNA梯状条带的形成。提示抗Fas单抗可能通过与SCC细胞表达的Fas抗原结合而介导细胞凋亡。

项目成果

Fujita, M.: "Induction of apoptosis in human oral squamous carcinoma cell lines by protein phsphatase inhibitors"European Journal of Cancer Oral Onoology. 35. 401-408 (1999)

Fujita, M.：“蛋白磷酸酶抑制剂诱导人口腔鳞状癌细胞系凋亡”欧洲癌症口腔肿瘤学杂志。

Morimoto, Y.: "The protein phosphatase inhibitors, okadaic acid and calyculin A, induce apoptosis in human submandibular gland ductal cell line HSG cells"Oral Diseases. 5. 10-110 (1999)

Morimoto, Y.：“蛋白磷酸酶抑制剂冈田酸和花萼蛋白 A 可诱导人颌下腺导管细胞系 HSG 细胞凋亡”口腔疾病。

Muraki Y., Yoshioka C., Tateishi A., Fukuda J., Haneji T.and Kobayashi N: "Localization of Fas antigen in oral squamous cell carcinoma"Brit. J. Oral Maxillofac. Surg. 37. 37-40 (1999)

Muraki Y.、Yoshioka C.、Tateishi A.、Fukuda J.、Haneji T. 和 Kobayashi N：“Fas 抗原在口腔鳞状细胞癌中的定位”Brit。

Muraki,Y.: "The significance of PCNA lebeling index at tumor invasion front in oral squamous cell carcinomas"The Asian Journal of Oral and Maxillofacial Surgery. 11. 41-46 (1999)

Muraki,Y.：“口腔鳞状细胞癌肿瘤侵袭前沿的 PCNA 标记指数的意义”亚洲口腔颌面外科杂志。

Yasuhiro Morimoto: "The protein phosphatase inhibitors, okadaic acid and calyculin A induce apoptosis in human submandibular gland cell line HSG cells" Oral Diseases. in press. (1999)

Yasuhiro Morimoto：“蛋白磷酸酶抑制剂、冈田酸和花萼蛋白 A 诱导人颌下腺细胞系 HSG 细胞凋亡”口腔疾病。