Development of new anticancer drugs and nanobubbles suitable for specific molecular target therapy using sonoporation

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We investigated the effects on cytotoxicity by combination of a novel HDAC inhibitor, Ky-2, and bubble liposomes on oral squamous carcinoma cells in vitro. Cell viability was significantly reduced by treatment with Ky-2 at 25 nM and bubble liposomes and ultrasoud, while it also led to augmentation of the proportion of cells in the sub-G1 phase and DNA fragmentation. In addition, immunoblot analysis revealed that Ky-2 enhanced the expression of apoptosis-related proteins. We made new bubble liposomes from Doxil(R) and investigated the effects on cytotoxicity by combination of this new Doxil(R) bubble liposome (encapsulated doxorubicin bubble liposomes, EDBL) and ultrasound in human gingival squamous cell carcinoma Ca9-22 cells. Results showed that the effect on cytotoxity was enhanced by a combination of EDBL and ultrasound.