Molecular epidemiological study for the invasive infections due to Group A streptococci

A族链球菌侵袭性感染的分子流行病学研究

• 批准号: 08672639
• 负责人: ICHIYAMA Satoshi
• 金额: $ 1.41万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672639/
• 关键词: Invasive Group A Streptococcal infections; Toxic shock-like syndrome; Pulsed-field gel electrophoresis; Streptococcal pyrogenic exotoxin; spe gene; Dendrogram; Transmission of isolates among family members; A群β溶連菌; PFGE; 遺伝型; 血清型; SpeA; 交叉感染 /

In a survey of invasive streptococcal infections in Japan, we analyzed isolates of Streptococcus pyogenes collected between 1992 and 1994. Genomic DNA fingerprints produced by pulsed-field gel electrophoresis (PFGE) were compared by computer-assisted analysis. Among the 42 isolates from patients with invasive diseases, 16 PFGE-types were identified and genetic diversity was clearly demonstrated. Identical fingerprints were observed in both invasive and noninvasive isolates. Only 43% of invasive isolates produced streptococcal pyrogenic exotoxin A (SPE A) and 31% did not contain the spe A gene. These findings suggest that the dissemination of a specific clone is not sufficient to explain all cases of these diseases in Japan, and pose a question to the role of SPE A as a major virulent factor.We have also observed 4 cases of transmission of this organism among a patient with invasive disease and his or her family members. The clonal spread among the family members was confirmed by analyzing genomic DNA fingerprints of the isolates recovered from them. Although 14 persons resulted in only colonizers or self-limitted diseases, 3 developed invasive infections. Our findings indicate the need of antibiotic chemoprophylaxis for close contacts of patients with invasive streptococcal diseases.

在日本侵袭性链球菌感染的调查中，我们分析了 1992 年至 1994 年间收集的化脓性链球菌分离株。通过计算机辅助分析对脉冲场凝胶电泳 (PFGE) 产生的基因组 DNA 指纹进行了比较。在来自侵袭性疾病患者的 42 个分离株中，鉴定出 16 个 PFGE 型，并清楚地证明了遗传多样性。在侵入性和非侵入性分离株中观察到相同的指纹。只有 43% 的入侵分离株产生链球菌热原性外毒素 A (SPE A)，31% 不包含 spe A 基因。这些发现表明，特定克隆的传播不足以解释日本这些疾病的所有病例，并对 SPE A 作为主要毒力因子的作用提出了疑问。我们还观察到 4 例侵袭性疾病患者及其家人之间传播这种生物体的病例。通过分析从家庭成员中回收的分离株的基因组 DNA 指纹，证实了家庭成员之间的克隆传播。尽管 14 人仅导致定植或自限性疾病，但 3 人出现侵袭性感染。我们的研究结果表明，与侵袭性链球菌疾病患者的密切接触者需要使用抗生素进行化学预防。

项目成果

Ichiyama S,Nakashima K,Shimokata K,Ohta M,Shimizu Y,Ooe K,Igarashi H,and Murai T: "Transmission of Streptococcus pyogenes causing toxic shock-like syndrome among family members and confirmation by DNA macrorestriction analysis." Journal of Infectious Dise

Ichiyama S、Nakashima K、Shimokata K、Ohta M、Shimizu Y、Ooe K、Igarashi H 和 Murai T：“化脓性链球菌在家庭成员中传播导致中毒性休克样综合征，并通过 DNA 宏观限制分析进行确认。”

一山 智: "Transmission of Streptococcus pyogenes causing toxic shock-like syndrome among family members and confirmation by DNA macrorestriction analysis" Journal of Infectious Diseases. 125. 723-726 (1997)

Satoshi Ichiyama：“化脓性链球菌在家庭成员中传播引起中毒性休克样综合征，并通过 DNA 宏观限制分析进行确认”《传染病杂志》125. 723-726 (1997)。

一山 智: "Transmission of streptococcus pyogenes causing toxic shock-like syndrome among family members and confimation by DNA macrorestriction analysis" Journal of Infectious Diseases. 125. 723-726 (1997)

Satoshi Ichiyama：“化脓性链球菌在家庭成员中传播引起中毒性休克样综合征，并通过 DNA 宏观限制分析进行确认”《传染病杂志》125. 723-726 (1997)。