The research on formation of the cell membrane and establishment of the primary metabolism in the primitive earth environments

原始地球环境中细胞膜的形成和初级代谢建立的研究

• 批准号: 10304045
• 负责人: MURAE Tatsushi
• 金额: $ 23.34万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10304045/
• 关键词: primitive Earth environment; archaea; membrane lipid; cell membrain; biosynthesis; electrospray mass spectrometry; lysine; 化学進化; エーテル脂質; Thermplasma acidophilum; Sulfolobus sp.; 膜透過性; 古細胞; Thermoplasma acidophilum; Sulfolovus sp.; 有機化合物の膜透過

Distribution pattern of carbonaceous matter in Allende carbonaceous chondrite was examined in the imaging by the micro laser Raman spectroscopy in order to know present state of carbonaceous matter in the primitive Earth environment, and it was clarified that the difference existed for structure and present state of the carbonaceous matter between rim and matrix.The reaction of amino acid by the shock wave was examined in order to clarify the chemical evolution in primitive Earth to indicate the followings : 1) Though the carbonization of the particle surface progresses, when shock wave is applied to the solid particle of glycine, the reaction does not occur inside the solid particle of glycine. (2) It was clarified that the polymerization reaction progressed, when the shock wave was applied to freezing aqueous solution of glycine.In order to clarify the behavior of cell membrane analogs in the primitive Earth environments, the behavior of the amino acid in porous nylon sphere whose ho … More lls were covered with s film of phosphatidylcholine was examined, and it was clarified that the membrane permeability is affeted by the type of amino acid and the difference of the ionic state of the inside.The technique using the electro spray ionization mass spectrometry was developed as the convenient technique which analyzed the structure of the membrane lipid of the archaea in order to know the property of the cell membrane in primitive Earth.The correlation between carbon dioxide concentration and chemical structure of the membrane lipid which the archaea produced on cultivation at high carbon dioxide concentration which imitated the primitive Earth environment was examined. Though the carbon dioxide concentration did not influence in the number of the five-member rings in the isoprenoid chain, it was clarified that the condition of the polar group was affected.The lysine whose specific position was labeled with deuterium was administered to the halophilic archaea. The uptake pathway of the lysine in biosynthetic pathway of membrane lipid of the halophilic archaea was clarified.The distribution of the archaea lipid in the sediment in the Kirishima hot spring area was examined in order to develop the means of environment estimation of the primitive Earth, and the paleotemperature was estimated. Less

利用显微激光拉曼光谱成像技术研究了阿连德碳质球粒陨石中碳质物质的分布规律，以了解原始地球环境中碳质物质的现状。通过对冲击波作用下氨基酸反应的研究，阐明了冲击波作用下氨基酸的化学反应机理1）虽然颗粒表面的碳化进行，但当冲击波施加到甘氨酸固体颗粒时，在甘氨酸固体颗粒内部不发生反应。(2)为了阐明细胞膜类似物在原始地球环境中的行为，研究了氨基酸在多孔尼龙球中的行为，其分子量为1000 - 10000，分子量为1000 - 1000，分子量为1000 - 100，分子量为100 - 100，分子量为 ...更多信息 检查用磷脂酰胆碱的S膜覆盖的LLS，阐明了古细菌细胞膜的通透性受氨基酸种类和内部离子状态的影响，并建立了电喷雾质谱技术，作为分析古细菌膜脂结构的简便技术，以了解古细菌细胞膜的原始性质研究了模拟原始地球环境的高浓度二氧化碳培养条件下古生菌产生的膜脂化学结构与二氧化碳浓度的相关性。虽然二氧化碳浓度对类异戊二烯链中五元环的数目没有影响，但明确了极性基团的状态受到影响。将特定位置用氘标记的赖氨酸给予嗜盐古菌。阐明了嗜盐古生菌膜脂生物合成途径中赖氨酸的摄取途径，研究了古生菌膜脂在雾岛温泉区沉积物中的分布，以发展原始地球环境评价手段，并对古温度进行了估算。少

项目成果

T.Murae: "Facile distinction of neutral and acidic tetraether lipids in archaea membrane by halogen atom adduct ions in electrospray ionization mass spectrometry"J. Mass Spectrom.. 37・2. 209-215 (2002)

T.Murae：“通过电喷雾电离质谱法轻松区分古细菌膜中的中性和酸性四醚脂质”J. 37・215（2002）。

T.Murae: "Fluorescent organic matter in carbonaceous chondrites"Adv. Space Res.. 24・4. 469-476 (1999)

T.Murae：“碳质球粒陨石中的荧光有机物”Adv. Space Res. 24・476（1999）

F.Kitajima: "Evaluating the thermal metamorphism of CM chondrites by using the pyrolytic behanior of carbonaceous macromolecular matter"Geochim. Cosmochim. Acta. 66・1. 163-172 (2002)

F. Kitajima：“利用碳质大分子物质的热解行为评估 CM 球粒陨石的热变质作用”，Cosmochim 66·1 (2002)。

T. Murae, R. Muraoka, and F. Kitajima,: "Examination of intact ether lipid components of thermoacidophile Sulfolobus sp. By ESI/TOF mass spectrometry"J. Mass Spectrom. Soc. Jpn.. 49. 195-200 (2001)

T. Murae、R. Muraoka 和 F. Kitajima，：“通过 ESI/TOF 质谱法检查嗜热嗜酸菌 Sulfolobus sp. 的完整醚脂质成分”J。

T.Murae: "Examination of intact ether lipid components of thermoacidophile Sulfolobus sp. by ESI/TOF mass spectrometry"J. Mass Specrom. Soc. Jpn. 49・5. 195-200 (2001)

T.Murae：“通过 ESI/TOF 质谱法检查嗜热嗜酸菌的完整醚脂质成分”Jpn. 195-200。