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The study for co-relationship between the oral functions and the bioactive materials in brain

口腔功能与脑生物活性物质相关性的研究

基本信息

批准号：
10307045
负责人：
MORIMOTO Toshifumi
金额：
$ 24万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

Some clinical observations and animal experiments have been suggested that the deficencyt of the oral function decrease the activities of brain function including learning and memory. However, the detail of this mechanism has not been clarified. In order to understand the relationship between brain and the oral function, we focused on the bioactive materials in brain, in this project.We obtained the findings in this study as follows ;1) To know the relationship between the monoamine and the activity of trigeminal motor function, properties of the hyperpolarizing spike afterpotential and the low-voltage activated Ca2+ channel were examined in vitro. It was found serotonine increased the excitability of the trigeminal motoneurons.2) By using the microdialysis method, the dopamine concentration in the putamen was found to increase significantly during mastication compared with resting period. And the bilateral 6-hydroxydopamine lesions of the nigrostriatal dopaminergic pathway induced the change of the movement pattern during intake behavior. These results suggested that the nigrostriatal dopaminergic pathway might play a role in the sequential progress during the mastication.3) When the experimental animals were induced chronic pain by making the inflammation in the temporomandibular joint or parotid gland, activities of neurons in the spinal trigeminal nucleus caudaris were recorded. The data indicated the peripheral and central sensitization occurred, and suggested the opioid might be involved in this sensitization.4) Using the experimental animals, in which masticatory behavior were restricted by giving them only soft food, c-fos expression in the dorsal horn were examined when inflammation weas induced in hindpaw. C-fos expression was decreased when that experimental animal were activated the masticatory function with hard food and wood stick. These results suggested the possibility that is enhancement of mastication evoked the secretion of the opioid.

一些临床观察和动物实验表明，口腔功能障碍会降低包括学习记忆在内的脑功能活动。然而，这一机制的细节尚未澄清。为了了解脑与口腔功能的关系，本课题以脑内生物活性物质为研究对象，主要研究结果如下：1）通过体外实验研究了三叉神经运动功能的超极化峰电位和低电压激活钙通道的特性，探讨了单胺类物质与三叉神经运动功能的关系。结果表明，咀嚼运动后，大鼠三叉神经运动神经元兴奋性增加。2）微透析法发现，咀嚼运动时壳核内多巴胺浓度较安静时显著增加。黑质纹状体多巴胺能通路的双侧6-羟多巴胺损伤引起摄食行为时运动模式的改变。提示黑质纹状体多巴胺能神经通路可能参与了咀嚼过程的时序性进展。3）通过颞下颌关节或腮腺炎症诱发慢性疼痛，记录三叉神经脊束核尾侧核神经元的活动。实验结果表明，大鼠后爪出现了外周和中枢性的致敏作用，提示阿片类物质可能参与了这种致敏作用。4）采用软食限制咀嚼行为的实验动物，在后爪诱发炎症时，观察大鼠后角c-fos表达。刺激硬食物和木棒咀嚼功能后，C-fos表达降低。提示咀嚼运动的增强可能引起阿片样物质的分泌。