权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study on dynamic mechanism of perforation on cancer cells at shock waves exposures

冲击波作用下癌细胞穿孔的动力学机制研究

基本信息

批准号：
10450071
负责人：
TAKAYAMA Kazuyoshi
金额：
$ 8.06万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10450071/
关键词：
Underwater shock waves Tissue model Holographic interferometry Numerical simulation Perforation Cancer cell Shock wave 値シミュレーション

项目摘要

Extracoporeal shock wave lithotripsy (ESWL), non-invasive removal of kidney stones, is one of the most peaceful applications of shock waves. As a collaboration project with Medical School of Tohoku University we have developed prototype lithotriptor which was approved by the Ministry of Health and Welfare. As a result of basic research, tissue damages during ESWL treatments have been clarified and we found a technology to damage living tissue in a controlled fashion. This technology is extended to cancer research with the combination of chemotherapy during which it was discovered punctuation on the cancer cells when shock waves were exposed on them. The mechanism of punctuation is found to be related with shock wave interaction with nonhomogeneity of a medium with local curvature. The goal of present research is to clarify the mechanism of the punctuation in details.Results obtained are summarized as following :(1) Optical flow visualization of shock/tissue interaction has been developed. However, it has a limitiation in its spatial resolution. In order to compensate it, we seek for model tissues which have analogous characteristics to living tissues, with which various analogue experiments have been conducted and the results have been numerically simulated.(2) Finite fringe holographic interferometry has been developed and Fourier fringe analysis was well established. This method allowed to identify a slight density change created by shock wave exposures and was found to be effectively extended further shock/tissue interaction studies.(3) Numerical methods have been developed to be compared with experiments. Interactions between shock waves and various gas liquid interfaces have been successfully simulated.(4) A vertcal gas gun has been designed and constructed with which micro-shock/tissue interaction will be investigated. This is a facility which can directly simulate shock induced perforation on model tissue surface.

体外冲击波碎石术（ESWL）是一种非侵入性的肾结石清除术，是冲击波最和平的应用之一。作为与东北大学医学部的合作项目，我们开发了原型碎石机，并获得了厚生省的批准。作为基础研究的结果，ESWL治疗期间的组织损伤已经得到澄清，我们发现了一种以受控方式损伤活组织的技术。这项技术被扩展到癌症研究与化疗的结合，在此期间，当冲击波暴露在癌细胞上时，发现了癌细胞上的标点符号。分析表明，爆轰产生的机理与激波与局部曲率介质的非均匀性相互作用有关。本论文的主要工作是对冲击波与生物组织相互作用的机理进行深入的研究，主要研究成果如下：（1）建立了冲击波与生物组织相互作用的光流场可视化方法。然而，它在空间分辨率上有局限性。为了补偿它，我们寻求具有与活组织相似特性的模型组织，用它进行了各种模拟实验，并对结果进行了数值模拟。(2)发展了有限条纹全息干涉术，并建立了傅里叶条纹分析。该方法允许识别冲击波暴露产生的轻微密度变化，并被发现可有效扩展进一步的冲击/组织相互作用研究。(3)数值方法已经发展到与实验进行比较。成功地模拟了冲击波与各种气液界面之间的相互作用。(4)设计并制造了一个垂直气枪，用于研究微冲击波与生物组织的相互作用。这是一种可以直接模拟冲击引起的模型组织表面穿孔的设备。