GROWTH HOROMON LOCALIZATION AND ITS BASE SEQUENCE IN BOVINE PANCREATIC

牛胰腺生长激素定位及其碱基序列

• 批准号: 10460134
• 负责人: TSUCHIYA Takeshi
• 金额: $ 3.14万
• 依托单位: ISHINOMAKI SENSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10460134/
• 关键词: BOVINE; GROWTH HORMONE; PANCREAS; GLUCAGON; IMMUNOHISTOCHEMISTRY; BASE SEQUENCE; RT-PCR; PCR

The two different pancreatic islets present in the bovine pancreas. The one is small islets consisting 4 endocrine cells. The other is large islets consisting insulin positive cells. The large islets increase in the cross area from fetus to parturition, decrease after birth and disappear after 6 month.The GRF localizes in pancreatic polypeptide cells. The GH material localizes in glucagon cells and large isles cells. IGF-I and IGF-II localize in glucagon cells.These results suggest that GRF and GH are brain-gut peptides, that GRF -GH system of hypothalamic pituitary system exists in bovine pancreas, and that the GH of large islets stimulates the rapid growth from fetus to infant in bovine.We design 42 primers for bovine pancreatic GRF and GH base on amino acid and base sequences of human, bovine, rat and chicken GRF and GH.Most of primer reaction designed was negative in bovine pancreas by RT-PCR method, but single positive reaction of GRF was recognized to a position of 500 bp by primer of human, bovine and rat mixed. The similar primer (type of human, rat and chicken mixed) designed for duck pancreatic GRF is also positive reaction in duck pancreas. And we recognized the single band of GH-cDNA in duck pancreas by primer of human, rat and chicken mixed.These results suggest that the base sequences of the pancreatic GH-cDNA and of GRF -cDNA are similar to them of rat

牛胰腺中存在的两种不同的胰岛。一种是由4个内分泌细胞组成的小胰岛。另一种是由胰岛素阳性细胞组成的大胰岛。大胰岛从胎儿到分娩时在横截面积内增加，出生后减少，6个月后消失，GRF定位于胰腺多肽细胞。GH物质定位于胰高血糖素细胞和大胰岛细胞。本研究根据人、牛、猪所设计的引物在牛胰腺组织中的RT-PCR检测结果大多数为阴性，而人、牛、大鼠混合引物在500 bp处检测到GRF的单一阳性反应。鸭胰腺GRF相似引物（人、大鼠、鸡混合型）在鸭胰腺中也呈阳性反应。用人、大鼠和鸡的混合引物在鸭胰腺组织中识别出单一的GH-cDNA条带，表明鸭胰腺GH-cDNA和GRF -cDNA的碱基序列与大鼠胰腺GH-cDNA和GRF -cDNA相似

项目成果

土屋 剛: "ウシ膵臓の成長ホルモン放出因子成長ホルモン、IGFの局在"栄養生理研究会報. 43. 57-67 (1999)

Tsuyoshi Tsuchiya：“生长激素释放因子、生长激素和 IGF 在牛胰腺中的定位”《营养生理学研究杂志》43. 57-67 (1999)。

T.TSUCHIYA: "LOCALIZATION OF GRF, GH, IGF IN BOVINE PANCREAS"NUTRITION AND PHSIOLOGY RESEARCH. 43. 57-67 (1999)

T.TSUCHIYA：“GRF、GH、IGF 在牛胰腺中的定位”营养和生理学研究。

土屋 剛: "ウシ膵臓の成長ホルモン放出因子,成長ホルモン,IGFの局在"栄養生理研究会報. 43・1. 57-67 (1999)

土屋刚：“生长激素释放因子、生长激素和IGF在牛胰腺中的定位”《营养生理学研究通报》43・1（1999）。