GROWTH HOROMON LOCALIZATION AND ITS BASE SEQUENCE IN BOVINE PANCREATIC

牛胰腺中存在两个不同的胰岛。一个是由4个内分泌细胞组成的小岛。另一种是由胰岛素阳性细胞组成的大胰岛。大的胰岛在胎儿至分娩的横断区增加，出生后减少，6个月后消失。GRF定位于胰多肽细胞。生长激素物质定位于胰高血糖素细胞和大的胰岛细胞。本研究根据人、牛、大鼠、鸡GRF和GH的氨基酸和碱基序列，设计了42条扩增牛胰腺GRF和GH的引物，结果表明GRF和GH均为脑肠肽，牛胰腺存在下丘脑-垂体系统的GRF-GH系统，大胰岛GH刺激牛从胎儿到婴儿的快速生长。针对鸭胰腺GRF设计的类似引物(人、鼠、鸡混合型)在鸭胰腺中也呈阳性反应。用人、大鼠和鸡的混合引物对鸭胰腺GH-cDNA单条带进行了识别，结果表明，胰腺GH-cDNA1和GRF-cDNA1个碱基序列与大鼠的GH-cDNA1个碱基序列相似

The two different pancreatic islets present in the bovine pancreas. The one is small islets consisting 4 endocrine cells. The other is large islets consisting insulin positive cells. The large islets increase in the cross area from fetus to parturition, decrease after birth and disappear after 6 month.The GRF localizes in pancreatic polypeptide cells. The GH material localizes in glucagon cells and large isles cells. IGF-I and IGF-II localize in glucagon cells.These results suggest that GRF and GH are brain-gut peptides, that GRF -GH system of hypothalamic pituitary system exists in bovine pancreas, and that the GH of large islets stimulates the rapid growth from fetus to infant in bovine.We design 42 primers for bovine pancreatic GRF and GH base on amino acid and base sequences of human, bovine, rat and chicken GRF and GH.Most of primer reaction designed was negative in bovine pancreas by RT-PCR method, but single positive reaction of GRF was recognized to a position of 500 bp by primer of human, bovine and rat mixed. The similar primer (type of human, rat and chicken mixed) designed for duck pancreatic GRF is also positive reaction in duck pancreas. And we recognized the single band of GH-cDNA in duck pancreas by primer of human, rat and chicken mixed.These results suggest that the base sequences of the pancreatic GH-cDNA and of GRF -cDNA are similar to them of rat