TREATMENT OF HERNIATED INTERVERTEBRAL DISCS WITH AUTOGENOUS CALPAIN PURIFIED FROM PATIENT'S OWN RED BLOOD CELLS

在CHYMOPAPAIN的CHECAGARA治疗中，有过敏反应和瘫痪等并发症的报道。要想获得更安全的治疗，还需要其他安全有效的酶。从患者自身红细胞中提纯的自体μ钙蛋白酶可能会消除过敏反应。本研究发现，正常浓度的脑脊液对CaPin的酶反应有抑制作用。钙蛋白酶的特异性内源性抑制物CalPASTATIN的分子量约为80 kDa。抑制脑脊液中钙蛋白酶活性的物质分子量可能小于10 kDa。脑脊液进行十二烷基硫酸钠-PAGE分析。免疫印迹法未见抗ALPATIN抗体阳性染色。脑脊液中抑制钙蛋白酶的物质可能与钙蛋白酶不同，提示鞘内意外注射μ钙蛋白酶治疗脑脊液时，脑脊液将失去其酶活性。自体μ钙蛋白酶有可能预防瘫痪等主要并发症之一。我们建议使用自体μ钙蛋白酶治疗腰椎间盘突出症是一种更安全的治疗方法。

ON THE CHEMONUCLEOLYSIS WITH CHYMOPAPAIN, COMPLICATIONS SUCH AS ANAPHYLACTIC REACTION AND PARALYSIS HAVE BEEN REPORTED. TO MEKE CHEMONUCLEOLYSIS A SAFER TREATMENT, OTHER SAFE AND POTENT ENZYMES HAVE BEEN NEEDED. AUTOGENOUS μCALPAIN AS PURIFIED FROM THE PATIENT'S OWN RED BLOOD CELLS MIGHT PRIVENT ANAPHYLACTIC REACTION. CALPAIN HAS BEEN SHOWN TO THE DEGRADATIVE ACTIVITY ON PROTEOGLYCAN OF HE INTERVERTEBRAL DISCS OF RABBITS.IN THE PRESENT STUDY, WE FOUND CEREBROSPINAL FLUID INHIBIT ENZYME REACTION OF CALPAIN IN ITS NORMAL CONCENTRAION. THE MOLECULAR WEIGHT OF CALPASTATIN, SPECIFIC ENDOGENOUS INHIBITOR OF CALPAIN, IS APPROXIMATELY 80-kDa. THE MOLECULAR WEIGHT OF THE SUBSTANCE WHICH INHIBIT CALPAIN ENZYME ACTIVITY IN CEREBROSPINAL FLUID MIGHT BE LESS THAN 10 -kDa. CEREBROSPINAL FLUID WERE SUBJECTED TO SDS-PAGE. IN THE IMMUNOBLOTTING WITH ANTICALPASTATIN ANTIBODY POSITIVE STAINING WAS NOT OBTAINED. THE SUBSTANCE WHICH INHIBIT CALPAIN IN CEREBROSPINAL FLUID MIGHT BE DIFFERENT FROM CALPASTATIN.THESE DATA SUGGEST IN CASE OF ACCIDENTAL INTRATHECAL INJECTION OF μCALPAIN FOR CHEMONUCLEOLYSIS, ITS ENZYME ACTIVITY WILL BE LOST BY CEREBROSPINAL FLUID. IT IS POSSIBLE THAT AUTOGENOUS μCALPAIN PREVENT FROM ONE OF MAJOR COMPLICATIONS SUCH AS PARALYSIS. WE SUGGEST IT MAKES CHEMONUCLEOLYSIS WITH AUTOGENOUS μCALPAIN A SAFER TREATMENT OF HENIATED INTERVERTEBRAL DISCS.