The role of growth factors on wound healing of maxillofacial bone

生长因子对颌面部骨创面愈合的作用

• 批准号: 10470372
• 负责人: TAKATO Tsuyoshi
• 金额: $ 7.62万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470372/
• 关键词: Maxillofacial bone; growth factor; wound healing of bone; 顎顔面; 下顎骨; 仮骨延長; 骨治癒; 顎整形力

Molecular basis of wound healing of bone affected by mechanical stress has not been studied well. Locally produced autocrine or paracrine factors regulate the wound healing process. Basic fibroblast growth factor (bFGF) has the potent effects on this process. We have shown here the new findings of bFGF.Fibroblast growth factor-2 (FGF-2) is known to stimulate bone formation and resorption indirectly. We investigated the direct action of on mature osteoclasts. FGF-2 (10-11M) exhibited maximum stimulation (1.9-fold) of pit formation in isolated rabbit osteoclast cultures, whereas in mixed cultures of bone cells, FGF-2 (10-9M) showed the 7.5-fold stimulation. FGF-2 up-regulated the phosphorylation of cellular proteins including p42/p44 mitogen-activated protein (MAP) kinase, and increased the kinase-activity of FGF receptor-1. These results suggest that FGF-2 acts directly on mature osteoclasts through activation of FGF receptor-1 and p42/p44 MAP kinase, causing the stimulation of bone resorption at physiological or pathological concentrations.

尚未很好地研究受到机械应力影响的骨骼伤口愈合的分子基础。局部产生的自分泌或旁分泌因子调节伤口愈合过程。基本成纤维细胞生长因子（BFGF）对此过程具有有效的影响。我们已经在这里展示了BFGF.FIBROSTRAST生长因子-2（FGF-2）的新发现已知可以间接刺激骨形成和吸收。我们研究了对成​​熟破骨细胞的直接作用。 FGF-2（10-11m）在分离的兔破骨细胞培养物中表现出最大的刺激（1.9倍），而在混合骨细胞的混合培养物中，FGF-2（10-9m）显示了7.5倍的刺激。 FGF-2上调了细胞蛋白的磷酸化，包括p42/p44有丝分裂原激活蛋白（MAP）激酶，并增加了FGF受体1的激酶活性。这些结果表明，FGF-2通过激活FGF受体1和p42/p44映射激酶直接作用于成熟的破骨细胞，从而导致在生理或病理浓度下刺激骨吸收。

项目成果

Takato T: "Time-related changes in a case torus palatinus"Jounal of Oral & Maxillofacial Surgery. 56(4). 492-494 (1998)

Takato T：“腭环病例中与时间相关的变化”口腔杂志

高戸 毅: "骨トランスポート法による顎関節形成術に関する実験的研究-第2報 : 形態学的検討"日形会誌. 18,2. 83-91 (1998)

Takeshi Takato：“使用骨运输方法进行颞下颌关节置换术的实验研究 - 第 2 次报告：形态学研究”Journal of the Nichiga Kaikai 18,2（1998）。

Yonehara Y. Matsumoto S. Takato T. Harii K. Nakatsuka T.: "Functional Recovery of Reconstructed Mandibule using Gradual Distraction Technique and Dental Implantation"The Japanese Journal of plastic and Reconstructive Surgery. Vol. 42, No. 3. 211-220 (1999

Yonehara Y. Matsumoto S. Takato T. Harii K. Nakatsuka T.：“使用渐进牵引技术和牙科植入术重建下颌骨的功能恢复”日本整形重建外科杂志。

Takato T. Mori Y. Eguchi T. Matsumoto S.: "Applications of Bone Distraction Technique in the Maxillofacial Region"Hosp. Dent.. 10. 2-17 (1999)

Takato T. Mori Y. Eguchi T. Matsumoto S.：“骨牵引技术在颌面部区域的应用”Hosp。

高戸 毅: "Vascularized bone graft"形成外科. 第42巻. 277-285 (1998)

Takeshi Takato：“血管化骨移植”整形外科。 42. 277-285 (1998)