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DNA analysis from teeth by capillary electrophoresis using Genetic Analyzer

使用遗传分析仪通过毛细管电泳对牙齿进行 DNA 分析

基本信息

批准号：
10470453
负责人：
KOMURO Toshinobu
金额：
$ 9.47万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470453/
关键词：
Polymorphism Y-chromosome STR Sequence Repeat structure PCR Dental pulp Forensic odontology polymorphism Polymor phism DYS389 Direct sequencing Repeat structure Polymerase chain reaction (PCR)唾液

项目摘要

DNA typing of Y-chromosomal STR is useful in sexual crimes and paternity tests. In this study, we describe the allele frequencies of DYS19, DYS389, DYS390 and DYS391 loci in 154 Japanese males and the repeat structures of these loci. Each locus in all samples was detected by denaturing polyacrylamide gel electrophoresis and silver staining. In the DYS389 locus, a pair of primers give two amplification prodicts (DYS389 I and DYS389 II). Repeat structure was examined by using an ABI PRISMィイD1TMィエD1 310 Genetic analyzer (PE Applied Biosystems).Alleles 13-17, alleles 8-12, alleles 24-29, alleles 22-26, and alleles 9-11 appeared in the DYS19, DYS389 I, DYS389 II, DYS390 and DYS391 loci, respectively. Gene diversities (GD) as a measure for the Y-STR locus informativeness were 0.704, 0.654, 0.761, 0.749, and 0.307, respectively. By the DNA typing of 4 Y-STR loci, 95 haplotypes appeared in 154 samples. Most frequently haplotype appeared in 10 samples typed of DYS19 : allele 17, DYS389 I : allele 11, DYS389 II : allele 28, DYS390 : allele 25, and DYS391 : allele 10.Repeat structure and number of repeats in each locus are examined. The repeat structure in the DYS389 II locus was (CTGT)ィイD2pィエD2 (CTAT)ィイD2qィエD2 (CTGT)ィイD23ィエD2 (CTAT)ィイD2rィエD2. Alleles were related to the repeat numbers of p+q+r (p=4, 5 q=10-14, r=8-12). DYS389I alleles were related to the repeat numbers of r. The repeat structure in the DYS390 locus was (CTGT)ィイD2mィエD2 (CTAT)ィイD2nィエD2 (CTGT)ィイD21ィエD2 (CTAT)ィイD24ィエD2, and each allele was related to the repeat numbers of m+n+5 (m=8, 9, n=9-12). The repeat structures in the DYS19 and DYS391 loci were (CTAT)ィイD2mィエD2 (CTAC)ィイD21ィエD2 (CTAT)ィイD23ィエD2 and (CTAT)ィイD2mィエD2, respectively. DYS19 alleles were related to the repeat numbers of m+4 (m=9-13), and DYS391 alleles were related to m (m=9-11).Each allele in these loci had no different structure in the same DNA size.

Y染色体STR的DNA分型在性犯罪和亲子鉴定中很有用。本研究描述了154名日本男性DYS 19、DYS 389、DYS 390和DYS 391基因座的等位基因频率及其重复序列结构。采用变性聚丙烯酰胺凝胶电泳和银染法对所有样本中的每个位点进行检测。在DYS 389位点，一对引物可扩增出DYS 389 Ⅰ和DYS 389 Ⅱ两个片段。在DYS 19、DYS 389 I、DYS 389 II、DYS 390和DYS 391位点分别出现等位基因13-17、8-12、24-29、22-26和9-11。Y-STR基因座信息量的基因差异度（GD）分别为0.704、0.654、0.761、0.749和0.307。4个Y-STR基因座的DNA分型结果显示，154例样本共出现95种单倍型。在10个样本中，最常见的单倍型为DYS 19：等位基因17，DYS 389 I：等位基因11，DYS 389 II：等位基因28，DYS 390：等位基因25，DYS 391：等位基因10。DYS 389 II基因座的重复结构为（CTGT）重复D2 p重复D2（CTAT）重复D2 q重复D2（CTGT）重复D23重复D2（CTAT）重复D2 r重复D2。等位基因与p+q+r重复数有关（p=4，5 q=10-14，r=8-12）。DYS 389 I等位基因与r. DYS 390基因座的重复结构为（CTGT）重复D2 m重复D2（CTAT）重复D2 n重复D2（CTGT）重复D21重复D2（CTAT）重复D24重复D2，每个等位基因与重复数m+n+5有关（m=8，9，n=9-12）。DYS 19和DYS 391基因座的重复序列结构分别为（CTAT）重复D2 m重复D2（CTAC）重复D21重复D2（CTAT）重复D23重复D2和（CTAT）重复D2 m重复D2。DYS 19等位基因与m+4（m=9-13）重复数相关，DYS 391等位基因与m（m=9-11）重复数相关，在相同的DNA大小下，各等位基因的结构无差异。