CLARIFICATION OF MECHANISM OF SUBMERGENCE-TOLERANCE USING A SUBMERGENCE-TOLERANT RICE CULTIVAR

利用耐淹水稻品种阐明耐淹机制

• 批准号: 10490019
• 负责人: TANAKA Kiyoshi
• 金额: $ 6.46万
• 依托单位: TOTTORI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10490019/
• 关键词: active oxygen; anaerobic stress; ascorbate; differential display; giberellin synthesis; hydrogen peroxide; submergence stress; superoxide dismutase; 遺伝子発現; 冠水耐性イネ; グルタチオン; アスコルビン酸ペルオキシダーゼ; イネ; カタラーゼ

The visual damage of submergence usually develops after desubmergence. A part of the damage may be caused by active oxygen produced in transition of oxygen environment. The submergence-intolerant rice cultivar, IR42 suffered from more lipid peroxidation with the production of HィイD22ィエD2OィイD22ィエD2 than submergence-tolerant cultivar, FR13A, after desubmergence. The activities of active oxygen scavenging enzymes in both cultivars were about the same or higher relative to the nonsubmerged control. After 6d of submergence, the total ascorbate levels in both cultivars decreased by 81% and 55% for IR42 and FR13A, respectively. During 7d of recovery after desubmergence, however, FR13A increased level of total ascorbate up to 150% while IR42 did it up to only 78%. Moreover, the proportion of ascorbic acid to total ascorbate I FR13A increased significantly after 3d of recovery and reached the level of that in nonsubmerged control, but the proportion in IR42 was still low during the recovery phase. It means that FR13A could operate the AOS scavenging system better than IR42 after desubmergence. It is suggested that the AOS may be one of the causes of the damage after desubmergence and ascorbate may take ana active role in alleviating the damage.Three cDNA fragments were isolated by differential screening between FR13A desubmerged for 2days after seven days' submergence was cancelled, and non-submerged control FR13A. The one was a giberellin-synthesis-related gene, the second was a proteasome-related gene, and the third was a tolerant gene to pathogenic microorganisms.

浸没的视觉损害通常会在去尿后发生。损害的一部分可能是由氧气环境过渡时产生的活性氧引起的。浸入式水稻品种IR42与降级耐糊状品种FR13A相比，HII D22 OI D22的产生更大的脂质过氧化，在Desubmergence之后。相对于不接受对照，这两个品种中活性氧气清除酶的活性大致相同或更高。浸没6天后，IR42和FR13A的两个品种的总抗坏血酸水平分别降低了81％和55％。然而，在去激素后7天的恢复期间，FR13A的总抗坏血酸水平高达150％，而IR42的抗坏血酸水平最高为78％。此外，抗坏血酸与总抗坏血酸I FR13A的比例在恢复3D后显着增加，并达到了无材料控制中的水平，但是在恢复阶段，IR42的比例仍然很低。这意味着FR13A可以在Desubmergence后比IR42更好地操作AOS清除系统。 It is suggested that the AOS may be one of the causes of the damage after desubmergence and ascorbate may take ana active role in alleviating the damage.Three cDNA fragments were isolated by differential screening between FR13A desubmerged for 2days after seven days' submergence was cancelled, and non-submerged control FR13A.一个是与吉伯林合成相关的基因，第二个是与蛋白酶体相关的基因，第三个是对致病微生物的耐受基因。

项目成果

Tanaka,K., Igarashi,T., Aono,M. and Kubo,A.: "Photooxidative stress sensitivity of transgenic plants containing altered levels of active oxygen scavenging enzymes"In Stress Responses of Photosynthetic Organisms, (ed. by K. Sato and N. Murata) Elsevier, Am

田中，K.，五十岚，T.，青野，M.

Kubo,A., Aono,M., Nakajima,N., Saji,H., Tanaka,K. and Kondo,N.: "Differential Responses in activity of antioxidant enzymes to different environmental stresses in Arabidopsis thaliana"J. Plant Res.. 112. 279-290 (1999)

久保，A.，青野，M.，中岛，N.，佐治，H.，田中，K.

Kubo, A., Aono, M., Nakajima, N., Saji, H., Tanaka, J., and Kondo, N.: "Differential Response in activity of antioxidant enzymes to different environmental stresses in Arabidopsis thaliana"J. Plant Res.. 112. 279-290 (1999)

Kubo, A.、Aono, M.、Nakajima, N.、Saji, H.、Tanaka, J. 和 Kondo, N.：“拟南芥抗氧化酶活性对不同环境胁迫的差异响应”J。

Tanaka, J., Igarashi, T., Aono, M., and Kubo, A.: "Photooxidative stress sensitivity of transgenic plants containing altered levels of active oxygen scavenging enzymes. In Stress Responses of Photosyntheitc Organisms, pp.69-79"Ed. by K. Satoh and N. Murat

Tanaka, J.、Igarashi, T.、Aono, M. 和 Kubo, A.：“含有改变水平的活性氧清除酶的转基因植物的光氧化应激敏感性。光合生物的应激反应，第 69-79 页”

Terashima, I., Noguchi, K., Itoh-Nemoto, T., Park, Y., Kubo, A., and Tanaka, K.: "The cause of PSI Photoinhibition at low temperature in leaves of Cucumis sativus L., a chilling sensitive plant"Physiol. Plant. 103. 295-303 (1998)

Terashima, I.、Noguchi, K.、Itoh-Nemoto, T.、Park, Y.、Kubo, A. 和 Tanaka, K.：“黄瓜叶片低温下 PSI 光抑制的原因，